Unlock instant, AI-driven research and patent intelligence for your innovation.

Engineered artificial structure for vascular network generation and its construction method and application

A construction method and vascularization technology, which are applied in the field of engineered artificial structures and their construction to achieve the effects of excellent biological functions, in vivo regeneration, and wide use.

Active Publication Date: 2022-03-18
REGENOVO BIOTECH +1
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is still a lack of a large number of studies related to the assistance of natural vascular networks with normal functions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Engineered artificial structure for vascular network generation and its construction method and application
  • Engineered artificial structure for vascular network generation and its construction method and application
  • Engineered artificial structure for vascular network generation and its construction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Example 1 Preparation of vascularized growth factor sustained-release microspheres

[0099] 1. Sodium alginate (Sigma-Aldrich, A0682) was prepared into a 1.5% (w / w) solution with PBS buffer solution.

[0100] 2. Basic fibroblast growth factor (bFGF, Abcam, AB9596) was uniformly mixed with sodium alginate solution at a concentration of 2 ng / ml and then filled into a disposable needle tube with a volume of 10 ml.

[0101] 3. Connect the high-voltage electrostatic cell microsphere preparation device. The high-voltage electrostatic generating device adopts SA167-Y (Tianjin) high-voltage electric field generator with an output voltage of 15kV; clamp the 10ml syringe in step 2 on the propulsion device: LongerPump Company TS2-60 injection In the pump, the distance between the needle tip and the copper sheet is 20mm, the propulsion speed is 10ml / h, a needle with an inner diameter of 191μm is used, and the collector is a disposable plastic petri dish with a diameter of 60mm (pla...

Embodiment 2

[0104] The preparation of embodiment 2 hydrogel microspheres

[0105] 1. Mix a solution of sodium alginate (Sigma-Aldrich, A0682) and a solution of gelatin (Sigma-Aldrich, G1890) (solvent PBS buffer solution) to a final concentration of 2% (w / w) and 3% (w / w) respectively w) The hydrogel precursor solution.

[0106] 2. Uniformly mix vascular endothelial growth factor (VEGF, Abcam, AB9571) at a concentration of 10 ng / ml with the hydrogel precursor solution obtained in 1, and then fill it into a disposable needle tube with a volume of 10 ml.

[0107] 3. Connect the high-voltage electrostatic spray cell microsphere preparation device. The high-voltage electrostatic generator adopts SA167-Y (Tianjin) high-voltage electric field generator with an output voltage of 10kV; clamp the 10ml syringe in step 2 on the propulsion device: LongerPump Company TS2-60 In the syringe pump, the distance between the needle tip and the copper sheet is 10 mm, the propulsion speed is 20 ml / h, the needl...

Embodiment 3

[0110] Embodiment 3 The preparation of coating material coating layer

[0111]Mix 1ml of collagen solution (Sigma, C7661) with 1ml of hydrogel microspheres and put them into a centrifuge tube. At this time, the cell microspheres sink to the bottom due to their high density. Centrifuge at a speed of 1000r / min for 4min, so that the collagen solution is fully in contact with the unit structure microspheres at the bottom. Static culture in the centrifuge tube for 48-72 hours, so that the collagen solution is evenly distributed on the surface of the microspheres.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides an engineered artificial structure that can be used for vascular network generation and a construction method thereof. The artificial structure includes hydrogel microspheres, vascularized growth factor slow-release microspheres, vascularized cells and carrier materials. The artificial structure can be cultured in a bioreactor to obtain a blood vessel network, or transplanted into the body to regenerate the blood vessel network. The obtained vascular network has physiological functions and can be used for in vivo and in vitro studies such as angiogenesis, disease occurrence research, and drug detection. This technology is used in tissue engineering, regenerative medicine, in vitro physiological model / pathological model / pharmacological model construction, tissue / organ / human chip, cell biology or drug research, etc.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to an engineered artificial structure that can be used for vascular network generation and its construction method and application. Background technique [0002] Rapid and sufficient vascularization of engineered structures in vivo has always been a bottleneck in tissue engineering and regenerative medicine, because when the distance between each cell and the nearest blood vessel is greater than 200 μm, there is a problem of insufficient nutrient supply, which eventually leads to cell necrosis. In the absence of external conditions, the growth rate of blood vessels into the graft is extremely slow, generally on the order of a few millimeters per day. Therefore, for clinically meaningful grafts, the inclusion of a pre-placed functional vascular network is one of the important strategies to achieve successful vascularization and graft survival, remodeling, ie, an in vivo vasculari...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/20A61L27/22A61L27/24A61L27/50A61L27/52A61L27/54
CPCA61L27/3808A61L27/3826A61L27/3804A61L27/3834A61L27/20A61L27/222A61L27/24A61L27/50A61L27/52A61L27/54A61L27/507A61L2300/414A61L2300/602C08L5/04
Inventor 姚睿徐铭恩
Owner REGENOVO BIOTECH