A full-process biological detection device

Abstract

The invention discloses a full-process biological detection device, belonging to the technical field of biological detection, comprising at least one reactor, the reactor comprising at least one sample adding unit, at least one liquid controlled release unit, at least one extraction unit, at least one liquid Control cutting unit, at least one liquid storage unit, at least one liquid transfer unit, at least one liquid flow control unit, at least one liquid quantitative dispersion unit and multiple reaction pools, the above units are connected in a preset order through flow channels, when the When the reactor rotates around the rotation axis of the chip, the liquid in the reactor can flow from the upstream unit to the downstream unit through each flow channel under the centrifugal force or surface tension or the centrifugal force and the surface tension. The products provided by this solution have the characteristics of high integration, no need to add additional reagents, simple operation, stability and reliability, etc., achieving the goal of sealing the entire biological detection process and effectively avoiding cross-contamination between samples and equipment.

Description

Technical field [0001] The invention relates to the technical field of biological detection, in particular to a full-process biological detection device. Background technique [0002] Microfluidic chip technology is a technology that integrates basic operations such as sample preparation, separation, reaction and detection of various biological, chemical or medical analysis processes on a chip of a few square centimeters to tens of square centimeters. Because of its The characteristics of high integration, high degree of automation, and low consumption of samples and reagents have been widely used in many fields such as biochemical detection, nucleic acid amplification, immunoassay, cell sorting, food safety and environmental monitoring. [0003] Patent Document 1 (CN103831140A) proposes a microfluidic chip device for nucleic acid detection based on centrifugal drive, which solves the problems of automatic and accurate quantitative dispersion of liquid and physical isolation. By pr...

AI Technical Summary

Problems solved by technology

Judging from the specific technical solution given by the patent, its practicability and feasibility are not high

First of all, the three-fork pipe connecting the nucleic acid extraction unit, the PCR amplification unit and the waste liquid unit is just a simple three-fork structure. In practice, this simple three-fork structure is likely to cause liquid to deviate from the expected direction and enter the opposite side, whether it is Whether the waste liquid enters the nucleic acid recovery unit or the nucleic acid enters the waste liquid unit will have a serious impact on the subsequent reaction and lead to the failure of the entire experimental process.

Secondly, the patent mentions that nucleic acid adsorption and elution can be achieved at a relatively low rotational speed of 1500rpm, indicating that the solid-phase adsorption matrix is ​​in a loose state, and the efficiency of nucleic acid purification and recovery is low, which can easily lead to inability to effectively recover nucleic acids and cause downstream amplification reactions failed or invalid

In addition, the chip design does not include on-chip storage of wash and eluent, which is inconvenient for practical use, and the sequential release of liquid is very unreliable

[0007] It can be seen that the current existing technical solutions have the following problems: 1) the integration degree is low, the whole process integration cannot be realized, and more manual operations are still required to complete the complete biological detection; 2) the versatility is not enough, only It can handle specific types of samples, and cannot handle biological samples with slightly complex components. The scope of application is relatively limited and cannot meet the actual inspection needs; 3) The feasibility is not high, and some designs cannot pass reasonable scrutiny or practical verification; 4) Detection ability Poor, due to design compromises or deficiencies, its actual detection performance often cannot meet the needs; 5) Many other deficiencies, such as poor sealing and practicability, have higher requirements for operators or detection equipment, different There is a high risk of cross-contamination between samples or between samples and equipment, etc.

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