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Lactobacillus plantarum for generating acid inulase and application of lactobacillus plantarum for generating acid inulase

A technology of Lactobacillus plantarum and acid inulinase, applied in the direction of application, enzyme, bacteria, etc., to achieve high stability effect

Pending Publication Date: 2020-06-05
南宁中诺生物工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many microorganisms that can produce exo-inulinase in nature, such as bacteria, fungi, and yeasts. At present, Aspegillius sp., Penicillium sp., and Kluyveromyces are the most studied. (Kluyveromyces sp.), but there are few research reports on acid inulinase from food-grade microbial sources

Method used

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  • Lactobacillus plantarum for generating acid inulase and application of lactobacillus plantarum for generating acid inulase
  • Lactobacillus plantarum for generating acid inulase and application of lactobacillus plantarum for generating acid inulase
  • Lactobacillus plantarum for generating acid inulase and application of lactobacillus plantarum for generating acid inulase

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Experimental program
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Effect test

Embodiment 1

[0059] Embodiment 1 The cultivation and identification of producing acid inulinase Lactobacillus plantarum 3-2 bacterial strain

[0060] (1) The cultivation of acid inulinase-producing Lactobacillus plantarum 3-2 bacterial strain

[0061] Take out the Lactobacillus plantarum 3-2 screened from the traditional raw rice flour of Guangxi Zhuang nationality in the laboratory from the ultra-low temperature refrigerator at -80 ℃, and inoculate it on the MRS solid medium (10g of beef extract, 5g of yeast powder, Peptone 10g, dipotassium hydrogen phosphate 2g, diammonium citrate 2.0g, sodium acetate 5.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, Tween-80 1mL, agar 20g, distilled water 1000mL, pH 6.6-6.8, 121℃ Autoclave for 15 minutes; add 2% glucose when used), cultivate at 30°C until a single colony appears; pick a single colony with an inoculation loop and inoculate it into a solid fermentation medium with inulin as the only carbon source (beef extract 10g , yeast powder 5g...

Embodiment 2

[0069] The application of a plant lactobacillus Lactobacillus plantarum 3-2 producing acid inulinase in the production of inulinase comprises the following steps:

[0070] (1) Seed cultivation

[0071] Streak the Lactobacillus plantarum 3-2 onto the MRS solid medium and culture it statically at 30°C until a single colony appears.

[0072] Described MRS solid medium formula is as follows:

[0073] Beef extract 10g, yeast powder 5g, peptone 10g, dipotassium hydrogen phosphate 2g, diammonium citrate 2.0g, sodium acetate 5.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, Tween-80 1mL, agar 20g, distilled water 1000mL , at pH 6.6-6.8, autoclaved at 121°C for 15 minutes;

[0074] 20% glucose stock solution: prepare 200g / L glucose solution with ultrapure water, and autoclave at 121°C for 20min;

[0075] Add glucose stock solution with a final concentration of 2% when used.

[0076] (2) Fermentation culture

[0077] Pick a single colony that normally grows in the solid mediu...

Embodiment 3

[0090] The application of a plant lactobacillus Lactobacillus plantarum 3-2 producing acid inulinase in the production of inulinase comprises the following steps:

[0091] (1) Seed cultivation

[0092] Streak Lactobacillus plantarum 3-2 into the MRS solid medium, and culture it statically at 30°C until a single colony appears; the formula of the MRS solid medium is as follows:

[0093] Beef extract 10g, yeast powder 5g, peptone 10g, dipotassium hydrogen phosphate 2g, diammonium citrate 2.0g, sodium acetate 5.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, Tween-80 1mL, agar 20g, distilled water 1000mL , autoclave at pH 6.6-6.8 and 121°C for 15 minutes;

[0094] 20% inulin stock solution: prepare 200g / L inulin solution with ultrapure water, and autoclave at 121°C for 20 minutes;

[0095] Add inulin stock solution with a final concentration of 2% when used.

[0096] (2) Fermentation culture

[0097] Streak the single colony in the MRS solid medium into the solid fermen...

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Abstract

The invention discloses lactobacillus plantarum 3-2 for generating acid inulase and an application of the lactobacillus plantarum 3-2 for generating acid inulase. The srtain is preserved in China Center for Type Culture Collection (CCTCC) on 25 November 2019, wherein the preservation number is CCTCC NO:M 2019972. The Lactobacillus plantarum 3-2 is screened from Guangxi Zhuang nationality traditional foods namely cold pressing rice powder, and in a fermentation medium that inulin is used as a carbon source and a beef extract is used as a nitrogen source, the lactobacillus plantarum 3-2 can growwell, and is high in acid resistance. After the acid inulase generated by the strain is placed under the condition that the pH is 4.5 for 1h, the survival rate is 96%, and the lactobacillus plantarum3-2 has the capacity for degrading inulin and levan substances and preparing high fructose syrup. For the first time, the lactobacillus plantarum for generating the acid inulase is reported, and a new strain and inulase thereof are provided for industrial degradation of the inulin and levan substances and preparation of the high fructose syrup.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the field of development of functional lactic acid bacteria and production and development of products thereof. In particular, it relates to a plant lactobacillus Lactobacillus plantarum3-2 producing acid inulinase and its application. Background technique [0002] Inulin is a kind of natural fructan mixture, which is composed of fructose furan linked by β-2,1-glucosidic bonds and a glucose linked at the reducing end. The degree of polymerization is generally 2-60. Inulin is widely distributed in the Compositae and Campanulaceae of dicotyledonous plants and Liliaceae and Gramineae of monocotyledonous plants, especially in Jerusalem artichoke and chicory, which account for more than 90% of the dry weight of carbohydrates, and are rich and excellent. Fructose-based renewable resources can be used to produce bioethanol, high fructose syrup, fructooligosaccharides, acetone-bu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/24C12P19/14C12P19/02C12R1/25
CPCC12N1/20C12N9/2402C12P19/14C12P19/02C13K11/00C12Y302/0108C12R2001/25C12N1/205
Inventor 王成华陈睿黄澄李晓明陆迪
Owner 南宁中诺生物工程有限责任公司
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