Preparation method of walnut protein peptide-selenium chelate for regulating intestinal flora
A technology for regulating intestinal flora and walnut protein, applied in the protein composition of vegetable seeds, protein components of waste, protein food processing, etc., can solve the problem of protein extraction rate and protein purity reduction, and limit protein dissolution in walnut meal , reduce the conversion rate of protein and polypeptide, etc., to achieve the effect of improving extraction rate and purity, improving emulsification phenomenon, and increasing conversion rate
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Embodiment 1
[0041] A preparation method for regulating the walnut protein peptide-selenium chelate of intestinal flora, comprising the following steps:
[0042] (1) After the walnut kernels are degreased by hydraulic cold pressing, the obtained walnut meal is crushed and passed through a 60-mesh sieve to obtain walnut meal powder, and then added to the walnut meal powder according to the ratio of material to liquid 1:3 Distilled water, after stirring evenly, add oil-degrading bacteria with 3% weight of walnut meal according to the weight percentage, after stirring evenly, place the first ultrasonic treatment at a frequency of 150W and a temperature of 28°C for 50min, and the first ultrasonic treatment ends The first fermentation treatment was carried out at 28°C for 6 hours to obtain the first fermentation material; wherein, the oil-degrading bacteria were composite bacteria composed of Pseudomonas aeruginosa, Bacillus amyloliquefaciens and Candida crassa species, the weight ratio of Pseu...
Embodiment 2
[0048] A preparation method for regulating the walnut protein peptide-selenium chelate of intestinal flora, comprising the following steps:
[0049] (1) After the walnut kernels are degreased by hydraulic cold pressing, the obtained walnut meal is crushed and passed through a 60-mesh sieve to obtain walnut meal powder, and then added to the walnut meal powder according to the ratio of material to liquid 1:5 Distilled water, after stirring evenly, add oil-degrading bacteria with 4% walnut meal weight according to the weight percentage, after stirring evenly, place the frequency at 220W and the temperature at 30°C for the first ultrasonic treatment for 55 minutes, and the first ultrasonic treatment ends The first fermentation treatment was carried out at 30°C for 9 hours to obtain the first fermentation material; wherein, the oil-degrading bacteria were composite bacteria composed of Pseudomonas aeruginosa, Bacillus amyloliquefaciens and Candida crassa species, the weight ratio ...
Embodiment 3
[0055] A preparation method for regulating the walnut protein peptide-selenium chelate of intestinal flora, comprising the following steps:
[0056] (1) After the walnut kernels are degreased by hydraulic cold pressing, the obtained walnut meal is crushed and passed through an 80-mesh sieve to obtain walnut meal powder, and then added to the walnut meal powder according to the ratio of material to liquid 1:6 Distilled water, after stirring evenly, add oil-degrading bacteria with 7% of the weight of walnut meal according to the weight percentage, after stirring evenly, place the frequency at 300W and the temperature at 38°C for the first ultrasonic treatment for 60 minutes, and the first ultrasonic treatment ends The first fermentation treatment was carried out at 38°C for 12 hours to obtain the first fermentation material; wherein, the oil-degrading bacteria were composite bacteria composed of Pseudomonas aeruginosa, Bacillus amyloliquefaciens and Candida crassa species, the w...
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