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Preparation method of walnut protein peptide-selenium chelate for regulating intestinal flora

A technology for regulating intestinal flora and walnut protein, applied in the protein composition of vegetable seeds, protein components of waste, protein food processing, etc., can solve the problem of protein extraction rate and protein purity reduction, and limit protein dissolution in walnut meal , reduce the conversion rate of protein and polypeptide, etc., to achieve the effect of improving extraction rate and purity, improving emulsification phenomenon, and increasing conversion rate

Pending Publication Date: 2020-06-12
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] Utilize biotechnology to carry out deep processing of protein resources on walnuts, and then use inorganic selenium for chelation to prepare polypeptide-selenium chelate, and transform inorganic selenium into organic selenium bioactive polypeptide-selenium chelate can significantly improve the absorption rate of selenium and utilization rate, in the technology disclosed in the current prior art, in addition to improving the human body's absorption rate and utilization rate of selenium, the characteristics of high fat content in walnuts have not been improved, mainly because walnut meal is processed by traditional degreasing methods. After degreasing treatment, walnut meal still contains a lot of oil, too much oil will lead to serious emulsification during subsequent protein extraction, which will greatly limit the dissolution of protein in walnut meal, resulting in a decrease in protein extraction rate and protein purity , so as to reduce the conversion rate of protein and polypeptide accordingly; it is also less found that there are other effects

Method used

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  • Preparation method of walnut protein peptide-selenium chelate for regulating intestinal flora

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A preparation method for regulating the walnut protein peptide-selenium chelate of intestinal flora, comprising the following steps:

[0042] (1) After the walnut kernels are degreased by hydraulic cold pressing, the obtained walnut meal is crushed and passed through a 60-mesh sieve to obtain walnut meal powder, and then added to the walnut meal powder according to the ratio of material to liquid 1:3 Distilled water, after stirring evenly, add oil-degrading bacteria with 3% weight of walnut meal according to the weight percentage, after stirring evenly, place the first ultrasonic treatment at a frequency of 150W and a temperature of 28°C for 50min, and the first ultrasonic treatment ends The first fermentation treatment was carried out at 28°C for 6 hours to obtain the first fermentation material; wherein, the oil-degrading bacteria were composite bacteria composed of Pseudomonas aeruginosa, Bacillus amyloliquefaciens and Candida crassa species, the weight ratio of Pseu...

Embodiment 2

[0048] A preparation method for regulating the walnut protein peptide-selenium chelate of intestinal flora, comprising the following steps:

[0049] (1) After the walnut kernels are degreased by hydraulic cold pressing, the obtained walnut meal is crushed and passed through a 60-mesh sieve to obtain walnut meal powder, and then added to the walnut meal powder according to the ratio of material to liquid 1:5 Distilled water, after stirring evenly, add oil-degrading bacteria with 4% walnut meal weight according to the weight percentage, after stirring evenly, place the frequency at 220W and the temperature at 30°C for the first ultrasonic treatment for 55 minutes, and the first ultrasonic treatment ends The first fermentation treatment was carried out at 30°C for 9 hours to obtain the first fermentation material; wherein, the oil-degrading bacteria were composite bacteria composed of Pseudomonas aeruginosa, Bacillus amyloliquefaciens and Candida crassa species, the weight ratio ...

Embodiment 3

[0055] A preparation method for regulating the walnut protein peptide-selenium chelate of intestinal flora, comprising the following steps:

[0056] (1) After the walnut kernels are degreased by hydraulic cold pressing, the obtained walnut meal is crushed and passed through an 80-mesh sieve to obtain walnut meal powder, and then added to the walnut meal powder according to the ratio of material to liquid 1:6 Distilled water, after stirring evenly, add oil-degrading bacteria with 7% of the weight of walnut meal according to the weight percentage, after stirring evenly, place the frequency at 300W and the temperature at 38°C for the first ultrasonic treatment for 60 minutes, and the first ultrasonic treatment ends The first fermentation treatment was carried out at 38°C for 12 hours to obtain the first fermentation material; wherein, the oil-degrading bacteria were composite bacteria composed of Pseudomonas aeruginosa, Bacillus amyloliquefaciens and Candida crassa species, the w...

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Abstract

The invention belongs to the technical field of food processing, and particularly relates to a preparation method of a walnut protein peptide-selenium chelate for regulating intestinal flora. The method comprises the following steps: (1) degreasing walnut kernels, and carrying out primary fermentation by using grease degrading bacteria to obtain a first fermentation material; (2) adding sucrose, serratia aromatica and zymomonas mobilis into the first fermentation material according to a certain ratio, and carrying out secondary fermentation to obtain a second fermentation material containing prebiotics; (3) carrying out enzymolysis on the second fermentation material by using a compound enzyme to obtain protein peptide; (4) adding selenium-enriched yeast into the obtained protein peptide to carry out selenium chelating treatment; and (5) carrying out pre-freezing, vacuum freeze-drying, packaging and sterilization on a selenium chelating material obtained in the step (4). In the invention, a selenium element can be provided for a human body, and a grease content is further reduced in a preparation process so that an extraction rate and purity of protein and a conversion rate of protein polypeptide are correspondingly improved, and an effect of regulating intestinal flora is also achieved.

Description

【Technical field】 [0001] The invention belongs to the technical field of food processing, and in particular relates to a preparation method of walnut protein peptide-selenium chelate for regulating intestinal flora. 【Background technique】 [0002] Walnut is one of the four major dried fruits in the world. Walnut is unattractive in appearance, but its nutritional value and medicinal value are very high. "Compendium of Materia Medica" records that walnut is warm in nature, sweet in taste, non-toxic, and has the functions of invigorating the stomach, nourishing blood, moistening the lungs, Rejuvenation and other effects. Modern scientific analysis shows that walnut kernel is rich in protein, various amino acids, trace elements, vitamins, etc., and is a rare and strong tonic. The main deep processing method of walnut is the production of walnut oil, which mainly adopts pressing method, which will produce a large amount of by-products—walnut meal, which is currently mainly used ...

Claims

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Application Information

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IPC IPC(8): A23L25/00A23L5/20A23L33/18A23L33/165A23J1/00A23J1/14A23J3/34
CPCA23L25/40A23L5/28A23L33/18A23L33/165A23J1/005A23J1/148A23J1/146A23J3/346A23V2002/00A23V2250/55A23V2250/548A23V2250/1626A23V2200/3202
Inventor 李明娟张雅媛游向荣王颖周葵卫萍黄展文
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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