Monascus fermentation product and aquatic functional biological feed thereof

A technology of monascus and fermented products, applied in the application field of monascus biological feed in aquaculture, can solve the problems of resource waste, environmental pollution, discarding, etc., and achieve the effects of promoting growth, enhancing immunity, and reducing cholesterol content

Pending Publication Date: 2020-06-19
福建大北农华有水产科技集团有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The by-products of pomelo (pomelo peel, pomelo residue and inferior pomelo, accounting for about 25%-40% of the total production) are often discarded during the production process, which not only causes waste of resources, but also causes environmental pollution

Method used

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  • Monascus fermentation product and aquatic functional biological feed thereof
  • Monascus fermentation product and aquatic functional biological feed thereof
  • Monascus fermentation product and aquatic functional biological feed thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The screening of embodiment 1 monascus

[0021] Source of the strain: It is taken from the Gutian area of ​​Fujian, and it is the starter (five types) used by the residents to make red distilled acid.

[0022] Isolation of Monascus:

[0023] Take two grains of different red yeast rice and crush them into powders, spread them on PDA medium with a tripod, incubate them at 30°C for 3-4 days, pick and shake the growing Monascus in sterile water test tubes, and further purify them by dilution plate method . The purified spore liquid of Monascus was added into a centrifuge tube containing sterilized glycerin and stored at -80°C.

[0024] Liquid fermented Monascus:

[0025] Add 10% Monascus spore liquid into 100mL liquid medium and cultivate at 30°C and 180 r / min for 10 days. The liquid medium formula is: 10% glucose, 1% peptone, 0.2% sodium nitrate, 0.2% potassium dihydrogen phosphate, Magnesium sulfate heptahydrate 0.05%, calcium chloride 0.01%.

[0026] The five strain...

Embodiment 2

[0043] Embodiment 2 monascus seed liquid culture

[0044] Optimization of seed liquid culture temperature:

[0045] Take the slant of the preserved Monascus test tube and put it into 5 500mL Erlenmeyer flasks containing 100mL liquid seed medium, and place them in culture at 26°C, 28°C, 30°C, 32°C and 34°C at a speed of 250r / min. Cultivate for 3 days in the shaker of the same method, measure the influence of different culture temperatures on the output of monascus pigment, the formula of liquid seed culture medium is: cornstarch 3.0%, cottonseed protein 1.0%, K 2 HPO 4 ·3H 2 O 1.0%, ZnSO 4 ·7H 2 O 1.0%, MgSO 4 ·7H 2 O 0.5%, pH 7.0.

[0046] Determination method of seed liquid color value:

[0047] Take 1 mL of the seed liquid and add it to a test tube containing 9 mL of 75% ethanol solution (pH 6.0-7.0) and shake well, centrifuge after 3 hours to take the supernatant, use 75% ethanol solution as a blank control, and measure light at 510nm and 410nm wavelengths. Absorpt...

Embodiment 3

[0054] The preparation of embodiment 3 pomelo peel Monascus fermented product

[0055] Fresh pomelo peels are air-dried and crushed into 2-5 mm grapefruit peel particles, and the crushed grapefruit peels are mixed with bran at a ratio of 3:1 to become the fermentation substrate of grapefruit peels. Inoculate the Monascus seed liquid prepared in Example 2 on the sterilized pomelo peel fermentation substrate with a water content of 35% at an inoculum size of 15%, mix evenly and spread it flat in a shallow pan and place it in a constant temperature room at 30°C for cultivation For 7 days, the humidity is controlled at 40%. After the solid-state fermentation is completed, it is dried by filter bed drying. The fermented material is pulverized to 60 mesh with a pulverizer.

[0056] Table 3 Results of solid fermentation

[0057] Before fermentation after fermentation Crude protein (%) 23.21 37.01 Crude fiber (%) 5.37 3.42 Moisture (%) 11.74 6.89 ...

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Abstract

The invention belongs to the field of biological feeds, and relates to monascus, a biological feed prepared by fermenting shaddock peel with the monascus, and application of the biological feed in aquaculture. According to the invention, the shaddock peel contains flavonoids, limonin and other active ingredients, and release of the substances can be promoted through fermentation. The monascus in the invention can generate a plurality of functional metabolites such as monascus pigment, protease, amylase, lipase, statins and the like, and a fermentation product contains small peptides. The biological feed is applied to aquaculture, and can remarkably improve the survival rate and immune function of aquatic animals, improve the growth performance, improve the body surface color of the aquaticanimals, and reduce cholesterol in muscles. The shaddock peel is used for the feed, so that the pollution of shaddock processing byproducts to the environment is reduced.

Description

technical field [0001] The invention belongs to the field of biological feed, and relates to a monascus strain and a biological feed prepared by fermenting pomelo peel, and the application of the monascus biological feed in aquaculture. Background technique [0002] Monascus fungi (Eumycophyta), Ascomycetes (Ascomycetes), Euascomycetes (Euascomycetes), Monascus (Monascus). Monascus is a saprophytic fungus, the optimum pH for growth is 3.5-5, it can tolerate pH 3.5, and it is especially fond of lactic acid. The growth temperature is 26-42°C, the optimum temperature is 32°C-35°C, and it can tolerate 10% ethanol. Monascus has attracted attention for its ability to produce a large amount of natural red pigment. [0003] Monascus has a long history of production and use in my country, and has high medicinal value. Monascus can produce a variety of enzymes, mainly including amylase, protease, esterase and cellulase. At present, monascus has been widely used in health care prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/12A23K10/37
CPCA23K50/80A23K10/12A23K10/37Y02P60/87Y02A40/818
Inventor 汪攀陈淑琼易敢峰孙云章王云爽李振江杨新冬李曼贠桂玲
Owner 福建大北农华有水产科技集团有限公司
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