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Zwitterionic polymer hydrogel as well as preparation method and application method thereof

A technology of amphoteric ions and polymers, applied in the direction of instruments, analytical materials, measuring devices, etc., can solve the problems of low measurement accuracy, achieve the effect of improving sensitivity and reducing the influence of errors

Active Publication Date: 2020-06-19
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The first object of the present invention is to overcome the defects of low measurement accuracy existing in the existing blood glucose detection technology, and provide a zwitterionic polymer hydrogel, which effectively immobilizes carbon quantum dots and glucose oxidase on the hydrogel Among them, the prepared hydrogel can respond and detect pH and glucose at the same time, improve the accuracy of blood glucose detection, and thus be more suitable for practical use and has industrial application value

Method used

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  • Zwitterionic polymer hydrogel as well as preparation method and application method thereof
  • Zwitterionic polymer hydrogel as well as preparation method and application method thereof
  • Zwitterionic polymer hydrogel as well as preparation method and application method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0068] A preparation method of zwitterionic polymer hydrogel, the specific preparation steps are:

[0069] S1. Add 1,2,4-triaminobenzene dihydrochloride and urea to 10mL ultrapure water to dissolve in a mass ratio of 1:1. After fully stirring, transfer the mixture solution to a polytetrafluoroethylene reactor Sealed, reacted at 220°C for 20 minutes, and naturally cooled to room temperature to obtain solution A;

[0070] S2. Filter the solution A in step S1 through a PTFE syringe filter with a pore size of 0.22 μm to remove large particles; then, dialyze the resulting suspension with ultrapure water for 36 hours, replace the ultrapure water every 6 hours, and the dialysis ends Finally, the solution is placed in a vacuum freeze-drying chamber to freeze-dry to obtain solid carbon quantum dots, which are stored for future use;

[0071] S3. Take: 2-methacrylic acid-tetrahydropyranyl ester solution, 2-(dimethylamino)ethyl methacrylate solution, and N,N'-methylenebisacrylamide are a...

Embodiment 2

[0076] A preparation method of zwitterionic polymer hydrogel, the specific preparation steps are:

[0077] S1. Add 1,2,4-triaminobenzene dihydrochloride and urea to 20mL ultrapure water to dissolve in a mass ratio of 2:3, and after fully stirring, transfer the mixture solution to a polytetrafluoroethylene reactor Sealed, reacted at 180°C for 60 minutes, and naturally cooled to room temperature to obtain solution A;

[0078] S2. Filter solution A in step S1 through a PTFE syringe filter with a pore size of 0.22 μm to remove large particles; then, dialyze the resulting suspension with ultrapure water for 24 hours, and replace the ultrapure water every 6 hours; the dialysis ends Finally, the solution is placed in a vacuum freeze-drying chamber to freeze-dry to obtain solid carbon quantum dots, which are stored for future use;

[0079] S3. Take methyl propane Sodium sulfonate the solution 、 Add 2-(dimethylamino)ethyl ethylene sulfonate solution and N,N'-methylenebisacrylamide ...

Embodiment 3

[0084] A preparation method of zwitterionic polymer hydrogel, the specific preparation steps are:

[0085] S1. Add 1,2,4-triaminobenzene dihydrochloride and urea to 15mL ultrapure water to dissolve in a mass ratio of 1:2. After stirring well, transfer the mixture solution to a polytetrafluoroethylene reactor Sealed, reacted at 240°C for 40 minutes, and naturally cooled to room temperature to obtain solution A;

[0086] S2. Filter solution A in step S1 through a PTFE syringe filter with a pore size of 0.22 μm to remove large particles; then, dialyze the resulting suspension with ultrapure water for 48 hours, and replace the ultrapure water every 8 hours; the dialysis ends Finally, the solution is placed in a vacuum freeze-drying chamber to freeze-dry to obtain solid carbon quantum dots, which are stored for future use;

[0087] S3. Take methyl propane sodium enophosphate the solution 、 Add 2-(dimethylamino)methylethylene phosphate ethyl ester solution and N,N'-methylenebisa...

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Abstract

The invention belongs to the technical field of polymer hydrogel preparation, and particularly relates to a preparation method of zwitterionic polymer hydrogel and an application of the zwitterionic polymer hydrogel in a biosensor. The zwitterionic polymer hydrogel provided by the invention comprises the following components: a zwitterionic polymer, carbon quantum dots and glucose oxidase, the preparation method of the zwitterionic polymer hydrogel is simple, and the application of the hydrogel in the field of biosensors is facilitated. The carbon quantum dots and the glucose oxidase are further encapsulated in the zwitterionic polymer hydrogel through hydrogen bond bonding to prepare pH-sensitive enzyme-containing hydrogel, so that the hydrophobic active sites of the enzyme can be effectively prevented from being interfered. A constructed biosensor can respond to and detect pH and glucose at the same time, and is wide in response range and high in sensitivity.

Description

technical field [0001] The invention belongs to the technical field of polymer hydrogel preparation, and in particular relates to a zwitterionic polymer hydrogel, a preparation method and an application method thereof. Background technique [0002] Currently in the development of biosensors, the long-term stability of the sensor used in vivo or in vitro is the most important performance index. After decades of research, the sensitivity, linear range, accuracy and other indicators of sensors have been greatly improved, but how to realize non-invasive blood glucose detection has become the direction that researchers are working hard for. In order to change this situation, in recent years, non-invasive measurement methods of blood glucose concentration are being actively studied, including the use of near-infrared spectroscopy, Raman spectroscopy, fluorescence and other optical means for blood glucose monitoring. The spectral signal-to-noise ratio of these methods is low, so t...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L43/02C08L41/00C08L33/14C08L89/00C08K3/04C08F220/28C08F220/34C08F222/38C08F228/02C08F230/02C08F2/50G01N27/30G01N27/327
CPCC08J3/075C08F220/28C08F220/34C08F228/02C08F230/02C08F2/50G01N27/302G01N27/3271G01N27/3272G01N27/308C08J2489/00C08K3/04C08J2343/02C08J2341/00C08J2335/00C08F222/385
Inventor 袁宁一江瑶瑶丁建宁戴胜平董旭
Owner CHANGZHOU UNIV
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