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Nuclear magnetic resonance hydrogen spectrum method for identifying whey protein powder added into formula powder

A technology of hydrogen nuclear magnetic resonance spectroscopy and whey protein powder, which is applied in the direction of using nuclear magnetic resonance for analysis, etc., can solve the problems of adding whey protein powder, not yet, etc.

Pending Publication Date: 2020-07-07
JIANGSU SINOGRAPHY TESTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a new type of detection method, proton nuclear magnetic resonance fingerprint technology has been used by many scholars in the study of the source and metabolites of dairy products, but it has not been used to identify whey protein powder added to formula powder. research report

Method used

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  • Nuclear magnetic resonance hydrogen spectrum method for identifying whey protein powder added into formula powder
  • Nuclear magnetic resonance hydrogen spectrum method for identifying whey protein powder added into formula powder

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Instruments, reagents and samples:

[0023] 1.1 Main instruments:

[0024] 400 MHz liquid nuclear magnetic resonance spectrometer and BBI probe from Bruker, Switzerland; IT0079 electronic balance from Mettler-Toledo Instruments (Shanghai) Co., Ltd.; 5mm nuclear magnetic tube from Wilmad-labglass in the United States; KH-500B ultrasonic cleaner; centrifuge from Shanghai Anting Scientific Instrument Factory.

[0025] 1.2 Reagents:

[0026] Sodium azide (NaN 3 ) purity specification is analytically pure, purchased from Tianjin Fuchen Chemical Reagent Factory; heavy water (D 2 O), the deuteration rate is 99.9%, purchased from Shanghai Anaiji Chemical Co., Ltd.; Methane (CHCl 3 ), the purity specification is analytically pure, purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd.; TSP with a mass fraction of 0.15% and NaN with a mass fraction of 0.015% 3 The mixed solution is prepared with heavy water.

[0027] 1.3. Sample collection

[0028] For whey prote...

Embodiment 2

[0037] 1. Instruments, reagents and samples:

[0038] 1.1 Main instruments:

[0039]400 MHz liquid nuclear magnetic resonance spectrometer and BBI probe from Bruker, Switzerland; IT0079 electronic balance from Mettler-Toledo Instruments (Shanghai) Co., Ltd.; 5mm NMR tube from Wilmad-labglass in the United States; Changzhou Guohua Electric Co., Ltd. HH-4 digital display water bath; centrifuge from Shanghai Anting Scientific Instrument Factory

[0040] 1.2 Reagents:

[0041] Sodium azide (NaN 3 ) purity specification is analytically pure, purchased from Tianjin Fuchen Chemical Reagent Factory; heavy water (D 2 O), the deuteration rate is 99.9%, purchased from Shanghai Anaiji Chemical Co., Ltd.; Methane (CHCl 3 ), the purity specification is analytically pure, purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd.; TSP with a mass fraction of 0.2% and NaN with a mass fraction of 0.015% 3 The mixed solution is prepared with heavy water.

[0042] 1.3. Sample collection...

Embodiment 3

[0052] 1. Instruments, reagents and samples:

[0053] 1.1 Main instruments:

[0054] 400 MHz liquid nuclear magnetic resonance spectrometer and BBI probe from Bruker, Switzerland; IT0079 electronic balance from Mettler-Toledo Instruments (Shanghai) Co., Ltd.; 5mm nuclear magnetic tube from Wilmad-labglass in the United States; KH-500B ultrasonic cleaner; centrifuge from Shanghai Anting Scientific Instrument Factory.

[0055] 1.2 Reagents:

[0056] Sodium azide (NaN 3 ) purity specification is analytically pure, purchased from Tianjin Fuchen Chemical Reagent Factory; heavy water (D 2 O), the deuteration rate is 99.9%, purchased from Shanghai Anaiji Chemical Co., Ltd.; Methane (CHCl 3 ), the purity specification is analytically pure, purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd.; TSP with a mass fraction of 0.12% and NaN with a mass fraction of 0.015% 3 The mixed solution is prepared with heavy water.

[0057] 1.3. Sample collection

[0058] The compound p...

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Abstract

The invention relates to a nuclear magnetic resonance hydrogen spectrum method for identifying whey protein powder added into formula powder. The method comprises the steps of weighing formula powderto be identified, dissolving with ultrapure water, adding a proper amount of trichloromethane, carrying out vortex centrifugation, measuring a small amount of the treated upper-layer sample, adding the small amount of the treated upper-layer sample into a heavy water solution containing 2,2,3,3-tetradeuterated trimethylsilane acrylic acid and sodium azide, uniformly mixing, and analyzing by usinga liquid nuclear magnetic resonance spectrometer. The nuclear magnetic resonance hydrogen spectrum fingerprint of the sample has the following characteristics: if a raised envelope peak exists withinthe displacement of 0.80-1.0ppm to be connected with zigzag multiple peaks, a cluster of multiple peaks exist within the displacement of 1.37-1.43ppm, and a peak exists within the displacement of 3.011-3.025ppm, it is proved that whey protein powder is added into the identified formula powder. The method provided by the invention is simple and efficient, and can accurately identify whey protein powder in formula powder such as bovine colostrum powder, formula milk powder and compound protein powder.

Description

technical field [0001] The invention belongs to the technical field of health food detection, and in particular relates to a method for identifying whey protein powder added in formula powder by using hydrogen nuclear magnetic resonance spectrum fingerprints. Background technique [0002] Whey protein is a protein extracted from milk, accounting for 20% of the total milk protein, mainly including β-lactoglobulin, α-lactalbumin, serum albumin and immunoglobulin. Whey protein has the advantages of high nutritional value, easy digestion and absorption, and rich in various biologically active ingredients. It plays an important role in the metabolic balance, immunity, and disease prevention of the human body. It is an important raw material of health food such as formula powder and sports nutrition supplement. However, some unscrupulous manufacturers do not add whey protein in the actual production of formula powder or add whey protein to some formula powder raw materials such a...

Claims

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Application Information

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IPC IPC(8): G01N24/08
CPCG01N24/08
Inventor 徐锦忠鲁惠玲石星宇
Owner JIANGSU SINOGRAPHY TESTING
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