Preparation and application of antibacterial nano-enzyme disinfection gel
A disinfectant gel and nano-enzyme technology, which is applied in the direction of antibacterial drugs, antifungal agents, cosmetic preparations, etc., can solve problems such as health hazards and adverse skin reactions, and achieve low dosage, environmental protection, biocompatibility, and sterilization Obvious effect
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Embodiment 1
[0022] 1. Preparation of nitrogen and chlorine-doped carbon dots (N / Cl-CDs) simulated oxidase: Weigh 1 part by weight of choline chloride, add 100 parts by weight of deionized water, mix and dissolve, add 0.3 parts by weight of urea, Sonicate for 10 min, transfer to a polytetrafluoroethylene reactor, heat at 200°C for 8 h, and after natural cooling, filter with a filter membrane with a pore size of 0.22 μm to obtain chlorine-nitrogen doped carbon dots, and vacuum-dry at 60°C for 24 h to obtain nanozyme.
[0023] 2. Nanozyme disinfection gel preparation: add 0.05 parts by weight of Carbomer-940 to 100 parts by weight of 75% ethanol to make it fully swell, add 0.01 parts by weight of nanozymes prepared in step 1, 0.1 parts by weight of hydrogen peroxide and 2 parts by weight of Parts of glycerol, stirred evenly, to obtain nano-enzyme disinfection gel.
[0024] 3. Accelerated investigation experiment of nano-enzyme disinfection gel:
[0025] Accelerated experiment: put the samp...
Embodiment 2
[0032] 1. Preparation of nitrogen and chlorine-doped carbon dots (N / Cl-CDs) simulated oxidase: Weigh 2 parts by weight of cetyltrimethylamine chloride, add 120 parts by weight of deionized water, mix and dissolve, add 0.5 Parts by weight of urea, ultrasonicated for 15 min, and transferred to a polytetrafluoroethylene reactor, heated at 200 °C for 10 h, cooled naturally, filtered with a filter membrane with a pore size of 0.22 μm, to obtain chlorine nitrogen doped carbon dots, and vacuum-dried at 60 °C After 24 hours, the nanozyme was obtained.
[0033] 2. Nanozyme disinfection gel preparation: add 0.06 parts by weight of Carbomer-940 to 100 parts by weight of 75% ethanol to make it fully swell, add 0.02 parts by weight of nanozymes prepared in step 1, 0.4 parts by weight of hydrogen peroxide and 3 parts by weight of Parts of butanediol, stir evenly, obtain nanozyme disinfection gel.
[0034] 3. Nanozyme disinfection gel accelerated investigation experiment: the same as in Exa...
Embodiment 3
[0038]1. Preparation of nitrogen and chlorine-doped carbon dots (N / Br-CDs) simulated oxidase: Weigh 3 parts by weight of cetyltrimethylamine bromide, add 150 parts by weight of deionized water, mix and dissolve, add 1 Parts by weight of urea, ultrasonicated for 20 min, and transferred to a polytetrafluoroethylene reactor, heated at 200°C for 12 h, cooled naturally, filtered with a filter membrane with a pore size of 0.22 μm to obtain chlorine-nitrogen doped carbon dots, and vacuum-dried at 60°C After 24 hours, the nanozyme was obtained.
[0039] 2. Nanozyme disinfection gel preparation: add 0.08 parts by weight of Carbomer-940 to 100 parts by weight of 75% ethanol to make it fully swell, add 0.03 parts by weight of nanozyme prepared in step 1, 0.3 parts by weight of hydrogen peroxide and 4 parts by weight of part betaine, and stir evenly to obtain nanozyme disinfection gel.
[0040] 3. Nanozyme disinfection gel accelerated investigation experiment: the same as in Example 1, t...
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