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Microorganism universal immobilized fermentation device and application thereof

A technology of immobilized fermentation and fermentation equipment, applied in the field of fermentation, can solve problems such as loss of energy consumption, easy winding of the stirring shaft, etc., and achieve the effects of low price, improved fermentation efficiency, and good universality

Pending Publication Date: 2020-07-28
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing traditional stirred fermenter directly puts the membrane into the fermenter, which is easy to wind the stirring shaft, and the external connection of the membrane device to the fermenter loses a lot of energy consumption, which is not suitable for immobilized cell fermentation.

Method used

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  • Microorganism universal immobilized fermentation device and application thereof
  • Microorganism universal immobilized fermentation device and application thereof
  • Microorganism universal immobilized fermentation device and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Microbial Universal Immobilization Fermentation Device

[0029] Such as figure 1 As shown, the fermentation device includes a fermenter body, and also includes a sampling port 1, a motor 2, a pH detection port 3, a defoamer 4, a metal frame 5, and a stirring device 6. The metal frame and the stirring device are combined together, and the metal The frame is made of stainless steel, the device is cylindrical, and the top is hollowed out. The materials required for immobilization and fermentation can be placed in the middle. The surrounding is a network structure, which can meet the full contact of the material with the medium. The size of the bottom hole is the stirring shaft of the fermentation tank. diameter, the upper and lower ends of the metal frame are respectively fixed by the stirring shaft and the defoamer.

Embodiment 2

[0031] The fermentation tank is detachably connected by the fermentation tank body and the metal frame, and the metal frame and the stirring device are combined together. When in use, the required base material for immobilized cells can be put into the metal frame, and the loading amount of the material is moderate, and it is best not to exceed the metal frame.

[0032] The immobilization material can be an inorganic material or an organic polymer material. The inorganic material includes but not limited to ceramics, silicon dioxide, alumina; the organic polymer material includes but not limited to polypropylene, polyethylene, polyethersulfone , polyvinylidene fluoride, etc.) flat sheet membrane, hollow fiber membrane, non-woven membrane, nanofiber membrane material; organic polymer material has the characteristics of good anti-microbial decomposition performance, high mechanical strength, stable chemical performance, etc., the following examples, select Polyvinylidene fluorid...

Embodiment 3

[0033] Example 3 Fermentation using polyvinylidene fluoride hollow fiber membrane as immobilized material

[0034] The unmodified / modified polyvinylidene fluoride hollow fiber membranes were put into metal frames respectively, and fermented in shake flasks. Before and after fermentation, they were washed with pure water and then dried in a freeze dryer for 6 hours, and their weights were measured. The results showed that the dry weight ratio of the modified polyvinylidene fluoride hollow fiber membrane was 8.5 times that of the unmodified one, which indicated that the modified polyvinylidene fluoride hollow fiber membrane was beneficial to the adsorption of bacteria.

[0035] For the modification method of modified polyvinylidene fluoride hollow fiber membrane, see patent CN 109847603 A, which adopts the surface coating modification technology mediated by biosurfactant, and the single factor experiment determines the best modification condition: at a temperature of 25 ℃, the r...

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Abstract

The invention discloses a microorganism universal immobilized fermentation device. The fermentation device comprises a fermentation tank body, a metal frame, a defoamer and a stirring device, whereinthe fermentation tank body is detachably connected with the metal frame; the metal frame and the stirring device are combined together; the metal frame is fixed by two ends of the stirring device; a film or a surface functionalized film is adopted as an immobilized material; the immobilized material is loaded into the metal frame; the metal frame is cylindrical; the upper part of the metal frame is hollow; a material required to be used for immobilized fermentation can be placed in the middle of the metal frame; and the periphery of the metal frame is of a network-shaped structure, so that thematerial can be in full contact with a culture medium; a hole is drilled in the bottom of the metal frame. The fermentation tank is formed by detachably connecting the fermentation tank body with themetal frame, so that the fermentation tank does not need to be integrally designed, can be additionally built, and is low in price, small in size, convenient to maintain and convenient to clean.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a fermentation device, which is used for the fermentation of microorganisms immobilized by membranes and surface functionalized membrane materials. Background technique [0002] Modern immobilized cell technology is developed under the impetus of immobilized enzyme technology. Karel et al. defined it as a new type of organism that fixes free cells in a confined space by chemical or physical means, reduces the impact of the environment on organisms, and keeps them as active as possible so that they can be used repeatedly and continuously. technology. Cell immobilization technology has the characteristics of small size, high efficiency, simple reaction operation, long-term maintenance of cell viability, repeated use, good stability, and strong tolerance. It has important applications in biochemical engineering, food engineering, environmental engineering, biomedi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/28C12M1/21C12M1/02C12M1/00
CPCC12M23/48C12M25/02C12M25/10C12M27/02C12M33/00C12M41/02C12M41/26
Inventor 姜岷方艳高豪信丰学马江锋章文明董维亮
Owner NANJING UNIV OF TECH
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