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Water-soluble composite immunoadjuvant and application thereof

A water-soluble composite and immune adjuvant technology, applied in vaccines, antiviral agents, multivalent vaccines, etc., can solve problems such as frequent stress responses of animals, reduce animal growth speed, increase veterinary workload, etc., and achieve tissue damage Effects of operations related to less stress response, extended storage time, and ease of mass production

Active Publication Date: 2020-07-31
内蒙古必威安泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] With the improvement of awareness of disease prevention and control, the current animal husbandry industry has switched from drug treatment to vaccine immunization for related diseases caused by bacteria. Therefore, the types of vaccines that animals need to be immunized have increased, which not only increases the workload of veterinarians, but also makes The increase in labor costs will also lead to frequent stress responses in animals and reduce the growth rate of animals

Method used

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  • Water-soluble composite immunoadjuvant and application thereof
  • Water-soluble composite immunoadjuvant and application thereof
  • Water-soluble composite immunoadjuvant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1 foot-and-mouth disease vaccine of the present invention

[0027] 1 Preparation of inactivated antigen

[0028] 1.1 Preparation of virus solution

[0029] 1.1.1 Preparation of type A foot-and-mouth disease virus liquid

[0030] 10000L bioreactor full suspension culture of BHK-21 cells, the cell density reaches 3-5×10 6 Inoculation of foot-and-mouth disease virus cell-adapted strain (bovine foot-and-mouth disease virus A / AKT-Ⅲ strain, prepared by Inner Mongolia Biwei Antai Biotechnology Co., Ltd.) according to the virus multiplicity of infection MOI 0.01-0.1, and the virus stock solution was prepared, and the stirring speed did not exceed 40rpm , cultivated for 8-12h to harvest the virus liquid.

[0031] 1.1.2 Preparation of O-type foot-and-mouth disease virus liquid

[0032] 10000L bioreactor full suspension culture of BHK-21 cells, the cell density reaches 3-5×10 6 When each / ml, inoculate foot-and-mouth disease virus cell-adapted strai...

Embodiment 2

[0060] Example 2 Foot-and-mouth disease compound water adjuvant inactivated vaccine efficacy detection

[0061] With 6-month-old FMDV antibody-negative calves, 10 calves were intramuscularly injected with 2 mL of A / AKT-Ⅲ strain foot-and-mouth disease compound water adjuvant inactivated vaccine in the neck, and blood was collected intravenously on the 28th day. ELISA test is used to judge the protectiveness of the vaccine by measuring the antibody titer.

[0062] Use about 40kg, 10 pigs that are negative for FMDV antibody, intramuscularly inject 2mL of O / Mya98 / XJ / 2010 strain foot-and-mouth disease compound water adjuvant inactivated vaccine behind each ear, and collect blood intravenously on the 21st day, and its serum is subjected to liquid phase resistance. ELISA test was used to judge the protection by measuring the antibody titer. The FMDV antibody titers of both pigs and cattle were greater than 1:128, indicating 99% protection.

Embodiment 3

[0063] Embodiment 3 The immune effect monitoring of the foot-and-mouth disease compound water adjuvant vaccine of the present invention

[0064] The diluted foot-and-mouth disease inactivated antigen and 206 adjuvant are mixed and emulsified according to the ratio of (V / V) 46:54 to prepare the foot-and-mouth disease oil adjuvant vaccine. Reference can be made to the patent application (Application No. 201510236068.4) titled "A Preparation Method for Foot-and-Mouth Disease Vaccine".

[0065] The O-type foot-and-mouth disease oil adjuvant vaccine (O / Mya98 / XJ / 2010 strain) prepared and the O-type foot-and-mouth disease compound water adjuvant inactivated vaccine prepared by 2.2 in embodiment 1 are respectively immunized with conventional dosage 30-45kg, and the FMDV antibody is Negative pigs were 8. The prepared type A foot-and-mouth disease oil adjuvant vaccine (A / AKT-Ⅲ strain) and the type A foot-and-mouth disease compound water adjuvant inactivated vaccine were immunized with ...

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Abstract

The invention provides a water-soluble composite immunoadjuvant and a foot-and-mouth disease composite water adjuvant inactivated vaccine. The water-solubility composite immunoadjuvant comprises 0.5-0.6% of carbomer,1%-3% of levamisole,1-2% of trehalose, 0.3-2% of raffinose, 0.5-1.5% of glucan, 0.5-1.5% of sorbitol, 0.5-1% of mannitol, 0.5-1.5% of xylitol, 0.2-2% of polyethylene glycol 3350, and 1-5% of D-alpha-Tocopheryl polyethylene glycol 1000 succinate. The water-solubility composite immunoadjuvant is free from mineral oil, easy to absorb by organisms, and small in tissue damage and stressreaction. The invention further relates to an application of the foot-and-mouth disease composite water adjuvant inactivated vaccine to freeze-dry vaccine combined immunization.

Description

technical field [0001] The present invention relates to the technical field of veterinary biological products, in particular to a water-soluble compound immune adjuvant, a foot-and-mouth disease compound water adjuvant inactivated vaccine comprising the water-soluble compound immune adjuvant, and its use in combined immunization with a freeze-dried vaccine application. Background technique [0002] Foot-and-mouth disease (FMD) is a severe contact infectious disease of cloven-hoofed animals caused by foot-and-mouth disease virus (FMDV), which mainly harms cattle, sheep, pigs, camels, etc., and has a very high incidence , spread extremely fast, and have great harm to the animal husbandry of various countries. Foot-and-mouth disease virus belongs to the Picornaviridae family and the genus Foot-and-mouth disease virus. Currently, there are 7 known serotypes, A, O, C, SAT1, SAT2, SAT3, and Asia1, and each serotype has many subtypes. Three serotypes are prevalent in my country,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/135A61K39/04A61K39/10A61K39/08A61K39/09A61K39/295A61P31/14A61P31/04
CPCA61K39/39A61K31/12A61K39/04A61K39/098A61K39/08A61K39/092A61P31/14A61P31/04A61K2039/55511A61K2039/55583A61K2039/552A61K2039/5252A61K2039/70Y02A50/30
Inventor 刘月张涛张晓慧马红艳武俊兰刘延麟李建丽
Owner 内蒙古必威安泰生物科技有限公司
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