Method for preparing glycyrrhetinic acid monoglucuronide by liquorice fermentation

A technology of glycyrrhetinic acid aldehyde and licorice aldehyde, which is applied in the field of microbial engineering, can solve problems such as affecting the consumption of GL by microorganisms, difficulty in product separation, and high price, and achieves suitable for large-scale production, simple operating parameters, and low production costs Effect

Active Publication Date: 2020-08-04
JIANGXI SCI & TECH NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reasons why the current biotransformation method cannot meet the industrial scale production of GAMG mainly include the following aspects: (1) Fermentation using GL as a substrate is not only expensive, but also GL has strong polarity and low water solubility, which affects the ability of microorganisms to GL consumption, thereby limiting microbial cell growth and enzyme production; (2) low biomass and enzyme production duri

Method used

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  • Method for preparing glycyrrhetinic acid monoglucuronide by liquorice fermentation
  • Method for preparing glycyrrhetinic acid monoglucuronide by liquorice fermentation
  • Method for preparing glycyrrhetinic acid monoglucuronide by liquorice fermentation

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Embodiment 1

[0036] Embodiment 1. A method for preparing glycyrrhetinic acid monoglucuronate by fermentation of licorice, comprising the following steps:

[0037] 1) Preparation of seed culture solution: Weigh 200g of potatoes, wash, peel and cut into small pieces, add water and boil for 20-30 minutes, filter with eight layers of gauze, add 20g of glucose, stir and mix well, and then make up water to 1000mL after cooling for a while , divided into Erlenmeyer flasks, stoppered and bandaged, sterilized at 121°C for 25 minutes, and then inserted into a plant endophytic fungus Chaetomium globosum DX-THS3 screened by our research team after cooling down, cultured at 28°C and 180rpm on a shaking table for 72h. Get the seed solution.

[0038] 2) Solid-state fermentation: dry licorice, crush it to 10 mesh, take 15g into a 250mL Erlenmeyer flask, mix it with 2g of wheat bran, add 50mL of culture medium (containing glucose 10g / L; ammonium nitrate 3g / L; KH 2 PO 4 2.2g / L; NaCl 0.5g / L; Magnesium Sul...

Embodiment 2

[0041] Embodiment 2, a method for preparing glycyrrhetinic acid monoglucuronate by fermentation of licorice, comprising the following steps:

[0042] 1) Preparation of seed culture solution: Weigh 200g of potatoes, wash, peel and cut into small pieces, add water and boil for 20-30 minutes, filter with eight layers of gauze, add 20g of glucose, stir and mix well, and then make up water to 1000mL after cooling for a while , divided into Erlenmeyer flasks, stoppered and bandaged, sterilized at 121°C for 25 minutes, and then inserted into a plant endophytic fungus Chaetomium globosum DX-THS3 screened by our research team after cooling down, cultured at 28°C and 180rpm on a shaking table for 72h. Get the seed solution.

[0043] 2) Solid-state fermentation: dry licorice, crush it to 10 mesh, take 15g into a 250mL triangular flask, mix with 2g of rice husk, add 50mL of culture medium (containing 10g / L of sucrose; 3g / L of peptone; KH 2 PO 4 2.2g / L; NaCl 0.5g / L; Magnesium Sulfate 0....

Embodiment 3

[0046] Embodiment 3, a method for preparing glycyrrhetinic acid monoglucuronate by fermentation of licorice, comprising the following steps:

[0047] 1) Preparation of seed culture solution: Weigh 200g of potatoes, wash, peel and cut into small pieces, add water and boil for 20-30 minutes, filter with eight layers of gauze, add 20g of glucose, stir and mix well, and then make up water to 1000mL after cooling for a while , divided into Erlenmeyer flasks, stoppered and bandaged, sterilized at 121°C for 25 minutes, and then inserted into a plant endophytic fungus Chaetomium globosum DX-THS3 screened by our research team after cooling down, cultured at 28°C and 180rpm on a shaking table for 72h. Get the seed liquid.

[0048] 2) Solid-state fermentation: dry licorice, crush it to 10 mesh, take 15g into a 250mL triangular flask, mix it with 2g of corn cob, add 50mL of culture medium (which contains fructose 10g / L; yeast powder 3g / L; KH 2 PO 4 2.2g / L; NaCl 0.5g / L; Magnesium Sulfat...

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Abstract

The invention discloses a method for preparing glycyrrhetinic acid monoglucuronide (GAMG) by solid-state fermentation of liquorice. The method comprises the following steps of: with Glycyrrhiza uralensis as a main component of a solid culture medium. inoculating Chaetomium globosumTHS3 to carry out solid-state culture, wherein after fermentation is finished, the conversion rate of glycyrrhizic acid (GL) in liquorice can reach 90% or above, so that a mixture containing the GAMG and GL is formed and can be used for preparing a mixed sweetening agent containing GAMG or a GAMG monomer sweetening agent, wherein higher sweetness and solubility are achieved. According to the method, cheap and medicinally and edibly homologous liquorice is directly used as a raw material for solid-state fermentation; the high-yield and high-purity GAMG can be obtained through extraction, separation and purification and concentration and drying of a fermentation product, a target product can be prepared withoutfirstly extracting and separating to obtain GL or preparing beta-D-glucuronidase in advance, the process steps are few, the production cost is low, no pollution is caused, and the method is suitablefor large-scale production.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular to a method for preparing glycyrrhetinic acid monoglucuronide through solid-state fermentation technology. Background technique [0002] Since excessive consumption of sugar can easily lead to dental caries, high blood pressure, diabetes and other diseases, it is an urgent need to find high-sweetness, low-calorie and safe natural sweeteners. Licorice is a plant of the genus Glycyrrhiza in the family Leguminosae, and is an important traditional Chinese herbal medicine with the same origin of medicine and food in my country. There are three original plants of licorice medicinal materials recognized by the "Chinese Pharmacopoeia", namely the roots and rhizomes of Ural licorice, bloated licorice and glabra. Currently, licorice extract is widely used around the world as a natural sweetener in food and beverages, as well as in pharmaceuticals and dietary supplements. Glycyrrhizic ac...

Claims

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Application Information

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IPC IPC(8): C12P33/20C12N1/14A23L27/10A61K8/63A61Q19/00A61K47/28C12R1/645
CPCC12P33/20C12N1/14A23L27/10A61K8/63A61Q19/00A61K47/28A61K2800/85A61K2800/10
Inventor 朱笃张迁肖依文汪涯高波良
Owner JIANGXI SCI & TECH NORMAL UNIV
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