Method for preparing glycyrrhetinic acid monoglucuronide by liquorice fermentation

Abstract

The invention discloses a method for preparing glycyrrhetinic acid monoglucuronide (GAMG) by solid-state fermentation of liquorice. The method comprises the following steps of: with Glycyrrhiza uralensis as a main component of a solid culture medium. inoculating Chaetomium globosumTHS3 to carry out solid-state culture, wherein after fermentation is finished, the conversion rate of glycyrrhizic acid (GL) in liquorice can reach 90% or above, so that a mixture containing the GAMG and GL is formed and can be used for preparing a mixed sweetening agent containing GAMG or a GAMG monomer sweetening agent, wherein higher sweetness and solubility are achieved. According to the method, cheap and medicinally and edibly homologous liquorice is directly used as a raw material for solid-state fermentation; the high-yield and high-purity GAMG can be obtained through extraction, separation and purification and concentration and drying of a fermentation product, a target product can be prepared withoutfirstly extracting and separating to obtain GL or preparing beta-D-glucuronidase in advance, the process steps are few, the production cost is low, no pollution is caused, and the method is suitablefor large-scale production.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular to a method for preparing glycyrrhetinic acid monoglucuronide through solid-state fermentation technology. Background technique [0002] Since excessive consumption of sugar can easily lead to dental caries, high blood pressure, diabetes and other diseases, it is an urgent need to find high-sweetness, low-calorie and safe natural sweeteners. Licorice is a plant of the genus Glycyrrhiza in the family Leguminosae, and is an important traditional Chinese herbal medicine with the same origin of medicine and food in my country. There are three original plants of licorice medicinal materials recognized by the "Chinese Pharmacopoeia", namely the roots and rhizomes of Ural licorice, bloated licorice and glabra. Currently, licorice extract is widely used around the world as a natural sweetener in food and beverages, as well as in pharmaceuticals and dietary supplements. Glycyrrhizic ac...

AI Technical Summary

Problems solved by technology

The reasons why the current biotransformation method cannot meet the industrial scale production of GAMG mainly include the following aspects: (1) Fermentation using GL as a substrate is not only expensive, but also GL has strong polarity and low water solubility, which affects the ability of microorganisms to GL consumption, thereby limiting microbial cell growth and enzyme production; (2) low biomass and enzyme production during fermentation lead to low GAMG production, which severely limits the industrial-scale application of GAMG; (3) only a few enzymes have GL hydrolysis activity, However, there are fewer enzymes that can selectively remove a distal glucuronic acid of GL to produce GAMG, and many of them will produce GA as a by-product. At the same time, it is difficult to separate the products, which limits the industrial scale production of GAMG.

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