Trivalent cerium ion coupled graphite phase carbon nitride and copper nano-cluster ratiometric fluorescent probe as well as preparation method and application thereof
A graphite-phase carbon nitride and ratiometric fluorescent probe technology, applied in the field of fluorescent sensing, achieves the effects of low detection limit, improved sensitivity, and fast response speed
Active Publication Date: 2020-08-11
WENZHOU MEDICAL UNIV
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Using cerium (III) ions as an inducer to promote the aggregation induction effect of copper nanoclusters and coupling graphitic carbon nitride to construct ratiometric fluorescent probes and use them for the sensitive detection of hydrogen peroxide and glucose has not yet been seen. to report
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Embodiment 1
[0031] Preparation of graphite-phase carbon nitride: 8.0 g of melamine was spread and dispersed in the center of a porcelain boat, then placed in a tube furnace, heated to 600 °C at a rate of 3 °C / min, and kept at this temperature for 2 hours. After cooling to room temperature, the resulting pale yellow blocky solid was ground into a powder in an agate mortar. Weigh 100mg of powdered carbon nitride and disperse it in 100mL of deionized water. Under 300W power, it is ultrasonically stripped for 8h and left standing overnight. The supernatant was taken and centrifuged at 5000 rpm for 30 min to obtain graphite phase carbon nitride. Depend on figure 1 It can be seen that the prepared graphitic carbon nitride is flake-like.
[0032] Preparation of copper nanoclusters: 10 mL of glutathione (50 mg / mL) was added dropwise to 10 mL of CuSO 4 (10 mM) in a round-bottomed flask (50 mL) at 37° C. for 1 hour at 650 rpm to generate a milky white suspension. Then use NaOH (1M) solution to ...
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Abstract
The invention discloses a trivalent cerium ion (cerium (III)) coupled graphite phase carbon nitride and copper nano-cluster ratiometric fluorescent probe as well as a preparation method and an application thereof, and belongs to the technical field of fluorescence sensing. Based on the phenomenon that hydrogen peroxide can enable fluorescence of a copper nano-cluster to be linearly quenched and has little influence on fluorescence of graphite-phase carbon nitride, a ratio type hydrogen peroxide and glucose fluorescence sensor taking the copper nano-cluster as a signal probe and the graphite-phase carbon nitride as a reference probe is constructed, and quantitative detection of hydrogen peroxide and glucose is realized. The metal cation cerium (III) is adopted to bridge the graphite phase carbon nitride and the copper nano-cluster, the fluorescence intensity of the glutathione-stabilized copper nano-cluster can be remarkably enhanced, and the sensitivity of the sensor is improved. The ratio type fluorescent probe based on the graphite phase carbon nitride-cerium (III)-copper nanocluster can avoid interference of factors such as instruments and environment, and has the characteristics of high response speed, wide linear range and low detection limit when being used for detecting hydrogen peroxide and glucose.
Description
technical field [0001] The invention belongs to the technical field of fluorescence sensing, and specifically refers to a trivalent cerium ion-coupled graphite phase carbon nitride and copper nano-cluster ratio fluorescent probe, a preparation method and application thereof. Background technique [0002] Hydrogen peroxide is one of the important reactive oxygen species, not only involved in the redox signaling pathway, but also an important biomarker in the process of protein folding, growth, signal transmission, differentiation and cell migration. The presence of hydrogen peroxide is of great significance in the process of regulating various biological activities, especially in the process of aging and carcinogenesis. It is worth noting that the disturbance or accumulation of intracellular hydrogen peroxide can lead to the development of several serious diseases, such as cancer and central nervous system diseases. Therefore, the detection of hydrogen peroxide is crucial fo...
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IPC IPC(8): C09K11/65C09K11/58G01N21/64
CPCC09K11/58C09K11/65G01N21/6428G01N2021/6432
Inventor 梅鹤王学东周佩佩黄宏廖忠鹭聂天银吕梦雨马伟
Owner WENZHOU MEDICAL UNIV