Freeze-drying protective agent capable of improving acid resistance of bifidobacteria and application of freeze-drying protective agent

A technology of freeze-drying protective agent and bifidobacteria, which is applied in the field of microorganisms, can solve the problems of activity loss, achieve the effects of low water content, improve acid resistance, and improve the survival rate of freeze-drying

Active Publication Date: 2020-08-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the emulsification method, the spray drying method solves the storage problem of the embedded product, and the process of the spray drying method is simpler, but, in the process of spray drying, the bifidobacteria will be affected by high temperature, which will produce great loss of activity

Method used

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  • Freeze-drying protective agent capable of improving acid resistance of bifidobacteria and application of freeze-drying protective agent
  • Freeze-drying protective agent capable of improving acid resistance of bifidobacteria and application of freeze-drying protective agent
  • Freeze-drying protective agent capable of improving acid resistance of bifidobacteria and application of freeze-drying protective agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Preparation of freeze-dried powder of Bifidobacterium bifidum

[0043] Specific steps are as follows:

[0044] Scheme 1: Configure freeze-dried protective agents 1-7 according to the formula in Table 1; dip the Bifidobacterium bifidum in the bacteria-preserving tube with an inoculating loop, streak it on the MRS-L solid medium, and cultivate it at 37°C for 24h , Get a single colony; pick a single colony and inoculate it in MRS-L liquid medium, and cultivate it at 37°C for 12h to obtain seed liquid; inoculate the seed liquid into fresh MRS-L at an inoculum of 4% (v / v) In a liquid medium, culture at 37°C for 12 hours to obtain a bacterial solution; centrifuge the bacterial solution at 8000g for 20 minutes to collect the bacterial sludge; resuspend the bacterial sludge in physiological saline to obtain a resuspension; use the resuspension as a blank control, After adjusting the pH of the resuspension to 6.5 (adjust the pH through a 3% NaOH solution with a mass volum...

Embodiment 2

[0053] Example 2: Preparation of Bifidobacterium adolescentis freeze-dried powder

[0054] Specific steps are as follows:

[0055] On the basis of Example 1, the Bifidobacterium bifidum was replaced by Bifidobacterium adolescentis, and the rhamnose was replaced by arabinose to obtain Bifidobacterium adolescentis freeze-dried powder 1-7 and Bifidobacterium adolescentis microcapsules (freeze). The dry protective agent formula is shown in Table 3).

[0056] Detect the number of viable Bifidobacterium adolescentis lyophilized powder 1~7 and Bifidobacterium adolescentis microcapsules, and calculate the number of Bifidobacterium adolescentis lyophilized powder 1~7 and Bifidobacterium adolescentis microcapsules. The freeze-dried survival rate of bifidobacteria (see Table 4 for test results).

[0057] Detect the number of viable Bifidobacterium adolescentis in the lyophilized Bifidobacterium adolescentis lyophilized powder 1~7 and Bifidobacterium adolescentis microcapsules treated with simul...

Embodiment 3

[0063] Example 3: Preparation of Bifidobacterium breve freeze-dried powder

[0064] Specific steps are as follows:

[0065] On the basis of Example 1, the Bifidobacterium bifidum was replaced by Bifidobacterium breve and the rhamnose was replaced by raffinose to obtain Bifidobacterium breve freeze-dried powders 1-7 and Bifidobacterium breve microcapsules ( The lyophilized protective agent formula is shown in Table 5).

[0066] Detect the number of viable Bifidobacterium breve in freeze-dried powder 1~7 of Bifidobacterium breve and Bifidobacterium breve microcapsules, and calculate the number of Bifidobacterium breve freeze-dried powder 1~7 and Bifidobacterium breve microcapsules. The freeze-dried survival rate of bifidobacteria (see Table 6 for test results).

[0067] Detect the number of viable Bifidobacterium breve in the lyophilized Bifidobacterium breve powder 1-7 and Bifidobacterium breve microcapsules treated with simulated gastric juice at pH 3.0 for 2 hours, and calculate the...

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Abstract

The invention discloses a freeze-drying protective agent capable of improving the acid resistance of bifidobacteria and an application of the freeze-drying protective agent, and belongs to the technical field of microorganisms. The invention provides the freeze-drying protective agent capable of remarkably improving the freeze-drying survival rate and the acid resistance of the bifidobacterial. The freeze-drying protective agent comprises a component A and a component B, wherein the component A is one or more of sodium alginate, sodium caseinate and sodium carboxymethylcellulose, and the component B is one or more of rhamnose, xylose, arabinose, mannose, sorbitol and raffinose. When the freeze-drying protective agent is used for preparing bifidobacterium freeze-dried powder, the freeze-drying survival rate of the bifidobacteria is as high as 65.68%, and after the bifidobacterium freeze-dried powder prepared from the freeze-drying protective agent is treated with simulated gastric juicewith the pH value of 3.0 for 2 h, the survival rate of the bifidobacteria is as high as 36.92%.

Description

Technical field [0001] The invention relates to a freeze-dried protective agent capable of improving the acid resistance of bifidobacteria and its application, and belongs to the technical field of microorganisms. Background technique [0002] Bifidobacterium is an important probiotic in the intestines of humans and animals. It has important physiological functions such as inhibiting the growth of pathogenic bacteria, regulating the intestinal microecological balance, enhancing nutrition, improving immunity, anti-tumor and delaying aging. However, bifidobacteria are obligate to anaerobic and are extremely sensitive to external adverse environmental factors such as oxygen, pH, temperature, and humidity. In addition, most bifidobacteria cannot resist the low pH of the human stomach (the pH of the human stomach is about 1.8 ~3.0), after taking bifidobacteria, it is difficult to ensure that enough live bacteria can reach the human intestine and exert its probiotic effect (the pH valu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12N1/20C12R1/01
CPCC12N1/04C12N1/20Y02A50/30
Inventor 赵建新崔树茂欧阳碧妍唐鑫毛丙永陆文伟翟齐啸陈卫张灏
Owner JIANGNAN UNIV
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