Method for detecting glucose based on cobalt-based metal organic framework enzyme-free glucose sensor

A glucose sensor and organic framework technology, applied in the field of glucose detection, can solve problems such as poor conductivity, and achieve the effects of simple equipment, fast and accurate detection, and fast detection speed.

Active Publication Date: 2020-08-14
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to its poor conductivity, other treatments are usually required
At present, there are...

Method used

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  • Method for detecting glucose based on cobalt-based metal organic framework enzyme-free glucose sensor
  • Method for detecting glucose based on cobalt-based metal organic framework enzyme-free glucose sensor
  • Method for detecting glucose based on cobalt-based metal organic framework enzyme-free glucose sensor

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Embodiment 1

[0048] A method for detecting glucose based on a cobalt-based metal organic framework enzyme-free glucose sensor, comprising the steps of:

[0049] (1) Preparation of ionic liquid [BMI]Br

[0050] Mix 0.138 mol of N-methylimidazole purified by distillation and 0.212 mol of degassed butyl bromide in a three-necked flask, reflux at 50°C, and react for 5 hours to obtain a light yellow oily ionic liquid [BMI]Br;

[0051] (2) Preparation of cobalt-based metal organic framework material Co-MOF

[0052] Take 1.00mmolCo(NO 3 ) 2 ·6H 2 O, 0.50 mmol H 2 BDC and 1mL [BMI]Br were mixed, placed in a 25mL reactor, and reacted at 140°C for 7 days to obtain Co-MOF, a cobalt-based metal-organic framework material;

[0053] (3) Preparation of modified electrodes

[0054] Polish the glassy carbon electrode on the suede with 1μm, 0.3μm and 50nm alumina in sequence, and then ultrasonically clean it with dilute nitric acid, absolute ethanol and deionized water to obtain a mirror-bright glassy...

Embodiment 2

[0066] A method for detecting glucose based on a cobalt-based metal organic framework enzyme-free glucose sensor, comprising the steps of:

[0067] The preparation of ionic liquid [BMI] Br, the preparation of Co-MOF carry out by embodiment 1,

[0068] (1) The ground Co-MOF (195 mg) was placed in 10 mL of 2M NaOH solution, stirred at room temperature for 1 h, then centrifuged at 8000 rpm, and finally dried at 70 °C for 12 h.

[0069] (2) Co(OH) obtained in step (1) 2 Put the powder in an agate mortar for grinding, then take 1.5mg in 1mL of absolute ethanol, and ultrasonicate for 20min to form a well-dispersed dispersion; take 10μL drop-coated on the polished glassy carbon electrode, and then drop 5μL Nafion (1% ) to fix.

[0070] (3) Test the current response value of the cobalt-based metal-organic framework enzyme-free glucose sensor to a known concentration of glucose solution at an applied potential of 0.6V. The concentration of the glucose solution is 5 μM to 900 μM, and ...

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Abstract

The invention relates to a method for detecting glucose based on a cobalt-based metal organic framework enzyme-free glucose sensor. The method is carried out by adopting the cobalt-based metal organicframework enzyme-free glucose sensor, and comprises the following steps: (1) establishing a three-electrode system by taking the cobalt-based metal organic framework enzyme-free glucose sensor as a working electrode; (2) fitting and drawing a calibration curve according to a current response value corresponding to glucose concentration; 3) determining the content of glucose in the to-be-determined solution. The method is based on the cobalt-based metal organic framework enzyme-free glucose sensor, and an electrochemical workstation and a three-electrode system are adopted for detection, so rapid and accurate detection of glucose is achieved through a chronoamperometry. The detection method is high in detection speed, stable current can be achieved within 5 seconds, and operation is easy and convenient. The detection limit is low (1.6 [mu]M), the linear range is wide (5-900 [mu]M), the influence of temperature and pH is avoided, the detection range is wide, and the sensitivity can reach 169 [mu]A*mM<-1>*cm<-2>.

Description

technical field [0001] The invention relates to a method for detecting glucose with an enzyme-free glucose sensor based on a cobalt-based metal organic framework, belonging to the field of glucose detection. Background technique [0002] The incidence of diabetes is gradually increasing, and it has become one of the chronic diseases that seriously threaten human health. Therefore, early diabetes prevention and diagnosis have attracted widespread attention. The glucose level in human blood is an important indicator of diabetes, so it is very important to monitor the glucose level accurately and quickly. Various methods have been used for glucose detection, such as fluorescence spectroscopy, high performance liquid chromatography, gas chromatography, and colorimetry. However, these methods require complex equipment and high costs, making them often difficult to commercialize. Electrochemical glucose sensors are being extensively studied due to their advantages of low cost a...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/30G01N1/34
CPCG01N1/34G01N27/301G01N27/308G01N27/416
Inventor 林猛张利军王南曹鹏飞
Owner SHANDONG UNIV
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