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A method for preparing protein small molecular peptides by simulating human digestive tract enzymatic hydrolysis

A technology for small molecular peptides and digestive tract, which is applied in the field of simulating human digestive tract enzymatic hydrolysis to prepare protein small molecular peptides, can solve the problems of unsuitable absorption and utilization, time-consuming enzymatic hydrolysis, inability to improve human immunity, etc. and enzymatic hydrolysis efficiency, improve enzymatic hydrolysis effect and efficiency, and promote the effect of full and uniform combination

Active Publication Date: 2020-12-25
营家健康科技(广东)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that at present, fresh animal bones are mainly hydrolyzed by a single enzymatic method, and polypeptide products produced by hydrolyzing a single enzyme or non-human digestive tract enzymes take a long time to enzymatically hydrolyze, the degree of enzymatic hydrolysis and the content of oligopeptides are low, and only by simple Stirring and mixing steps make it difficult to ensure that enzymes and proteins are fully and evenly combined, and the enzymatic hydrolysis effect and efficiency are poor. The relatively high molecular weight polypeptides in the enzymatic hydrolysis products account for a high proportion, which is not suitable for direct absorption and utilization by the human body, let alone improve the body's metabolism. immunity

Method used

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  • A method for preparing protein small molecular peptides by simulating human digestive tract enzymatic hydrolysis
  • A method for preparing protein small molecular peptides by simulating human digestive tract enzymatic hydrolysis
  • A method for preparing protein small molecular peptides by simulating human digestive tract enzymatic hydrolysis

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preparation example Construction

[0040] The present invention provides a method for simulating human digestive tract enzymatic hydrolysis to prepare protein small molecular peptides, the preparation method comprising the following steps:

[0041] Step 1, breaking the cleaned fresh animal bones into bone pieces, cooking them in boiling water, taking the bone pieces, drying and pulverizing them to obtain dry bone powder, adding distilled water to prepare a bone slime solution with a mass fraction of 20-30%;

[0042] Step 2, adjusting the pH value of the bone slurry to 3.0-5.0, adding papain and a reducing agent, supplemented by ultrasonic waves and microwaves for pre-enzymolysis, to obtain a pre-enzymolysis solution;

[0043] Step 3, filtering and centrifuging the pre-enzymolyzate obtained in step 2 to obtain a crude protein hydrolyzate;

[0044] Step 4, adjusting the pH value of the crude protein hydrolyzate to 1.0-4.0, adding pepsin and supplemented with ultrasonic waves to perform an enzymatic hydrolysis to ob...

Embodiment 1

[0062] Example 1: This example provides a method for simulating the enzymatic hydrolysis of the human digestive tract to prepare small protein peptides. The preparation method includes the following steps:

[0063] Step 1. Break 500g of washed fresh pig bones into bone pieces of about 3×3cm, add 1kg of boiling water and cook for 40min; after removing the surface oil of the cooking liquid, drain the bone pieces and dry them at 80°C to the water content 4.5%; then the dried bone pieces were coarsely pulverized and ultrafine pulverized in turn, passed through a 200-mesh sieve to obtain dry bone powder, and distilled water was added to prepare a bone slime solution with a mass fraction of 20%;

[0064] Step 2, adjust the pH value of the bone slurry to 3.0 with hydrochloric acid, add 2.5g of papain, and add 500mg of ascorbic acid at the same time, wherein the enzyme activity of papain is 800,000 U / g; 200W microwave irradiation for 5 minutes, supplemented by ultrasonic waves with a ...

Embodiment 2

[0071] Example 2: This example provides a method for simulating the enzymatic hydrolysis of the human digestive tract to prepare small protein peptides. The preparation method includes the following steps:

[0072] Step 1: Break 500g of washed fresh beef bones into bone pieces of about 3×3cm, add 1.25kg of boiling water and cook for 50min; The water content is 3.8%; then the dried bone pieces are coarsely pulverized and ultrafinely pulverized in turn, passed through a 200-mesh sieve to obtain dry bone powder, and distilled water is added to prepare a bone slime solution with a mass fraction of 25%;

[0073] Step 2: Use hydrochloric acid to adjust the pH value of the bone slurry to 4.0, add 2.5g of papain, and add 1.6g of ascorbic acid at the same time, wherein the enzyme activity of papain is 800,000 U / g; at 40°C, supplemented with power 200W microwave irradiation for 5 minutes, supplemented by ultrasonic waves with a power of 200W and a frequency of 50kHz to pre-enzymolyze th...

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Abstract

The invention discloses a method for preparing protein small molecule peptide by simulating human digestive tract enzymolysis, which comprises the following steps: crushing bones into bone blocks, cooking the bone blocks, drying and crushing the bone blocks, adding distilled water, and blending until the mass fraction is 20-30%; adjusting the pH value to 3.0-5.0, adding papain and a reducing agent, and carrying out pre-enzymolysis under the assistance of ultrasonic waves and microwaves; adjusting the pH value to 1.0-4.0, adding pepsase, and carrying out primary enzymolysis under the assistanceof ultrasonic waves; adjusting the pH value to 7.0-8.5, adding a mixed enzyme of chymotrypsin and trypsin, and carrying out secondary enzymolysis under the assistance of ultrasonic waves; carrying out desalination treatment; and collecting polypeptide liquid, and carrying out spray drying to obtain the protein small molecule peptide. On the basis of simulating an enzymolysis program of main digestive proteases in the digestive tract of a human body, a protein small molecule peptide product which is conveniently and directly absorbed and utilized by the human body is obtained through pre-enzymolysis, primary enzymolysis, secondary enzymolysis under assistance of ultrasonic waves and microwaves.

Description

technical field [0001] The invention relates to the field of production of small protein peptides, in particular to a method for preparing small protein peptides by simulating enzymatic hydrolysis of the human digestive tract. Background technique [0002] Animal bones are very rich in nutrients, including rich protein, fat and calcium. As the largest meat-eating country in the world, my country produces more than 15 million tons of bones every year. Therefore, making full use of animal bones and vigorously developing bone foods has become the food industry, especially the primary problem of meat processing enterprises. [0003] Scientific research in recent years has shown that not all proteins can be absorbed and utilized by the human body. The protein absorbed by the human body is mainly absorbed in the form of peptides. After protein is ingested into the human body, it needs to go through a specific and complex protein enzymatic process in the human digestive tract. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/18C07K1/34
CPCC07K1/18C07K1/34C12P21/06
Inventor 罗伟敏陈顺钦
Owner 营家健康科技(广东)有限公司