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Green fluorescent carbon quantum dot for detecting pH of acid environment and preparation method thereof

A technology of carbon quantum dots and green fluorescence, which is applied in the field of green fluorescent carbon quantum dots and its preparation, can solve the problems of unfriendly solvents, limited applications, and long time consumption, and achieve good water solubility and biocompatibility. Preparation method Simple, responsive effect

Inactive Publication Date: 2020-08-25
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most carbon dots emit blue fluorescence, which limits their applications in biology due to background interference.
The synthesis of carbon quantum dots mostly adopts hydrothermal method or solvothermal method, which takes a long time and the solvent is not friendly to the environment

Method used

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  • Green fluorescent carbon quantum dot for detecting pH of acid environment and preparation method thereof
  • Green fluorescent carbon quantum dot for detecting pH of acid environment and preparation method thereof
  • Green fluorescent carbon quantum dot for detecting pH of acid environment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation method of carbon quantum dots:

[0029] (1) Add 0.1081g of p-phenylenediamine, 195uL of phosphoric acid, and 96uL of ethylenediamine, dissolve in 5mL of secondary water, stir thoroughly and ultrasonically dissolve for 15 minutes;

[0030] (2) Put it into a microwave oven (1000w) and heat it for 3min10s;

[0031] (3) After cooling, add 5ml of ultrapure water and ultrasonically dissolve it; filter the product with filter paper and microfiltration membrane (0.22 μM) to remove macromolecular particles; centrifuge to obtain a dark brown solution, and freeze-dry the solution to obtain a dry brown powder. The relative quantum yield of the obtained carbon quantum dots was 2.6%.

[0032] Characterize the carbon quantum dots synthesized above:

[0033] Transmission electron microscope image of carbon quantum dots figure 1 . The carbon quantum dots present monodisperse spherical particles in morphology, and the particle diameter is about 1.35nm.

[0034] carbon qu...

Embodiment 2

[0042] (1) Add 0.1081g of p-phenylenediamine, 195μL of phosphoric acid, and 96μL of ethylenediamine, dissolve in 5mL of secondary water, stir thoroughly and ultrasonically dissolve for 15 minutes;

[0043] (2) Put it into a microwave oven (1000w) and heat it for 1min30s;

[0044] (3) After cooling, add 5mL of ultrapure water and ultrasonically dissolve it. The product was filtered with filter paper and microfiltration membrane (0.22 μM) to remove macromolecular particles. Centrifugation gave a dark brown solution, which was lyophilized to give a dry brown powder.

[0045] Compared with the carbon quantum dots obtained in Example 1, the obtained carbon quantum dots had an obvious blue shift in wavelength, and the relative quantum yield was 1.1%.

Embodiment 3

[0047](1) Add 0.1081g of p-phenylenediamine, 195μL of phosphoric acid, and 96μL of ethylenediamine, dissolve in 5mL of secondary water, stir thoroughly and ultrasonically dissolve for 15 minutes;

[0048] (2) Put it into a microwave oven (1000w) and heat it for 3min 40s;

[0049] (3) After cooling, add 5mL of ultrapure water and ultrasonically dissolve it. The product was filtered with filter paper and microfiltration membrane (0.22 μM) to remove macromolecular particles. Centrifugation gave a dark brown solution which was lyophilized to give a dry powder.

[0050] Compared with the carbon quantum dots obtained in Example 1, the obtained carbon quantum dots have no obvious change in wavelength and relative quantum yield, but it takes a long time and wastes resources.

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Abstract

The invention provides a green fluorescent carbon quantum dot for detecting pH of an acid environment as well as a preparation method and application of the green fluorescent carbon quantum dot. The preparation method comprises the following steps: putting p-phenylenediamine, phosphoric acid and ethylenediamine into a beaker, adding secondary water, fully stirring, and ultrasonically dissolving; putting the mixture into a microwave oven (600w-1000w) to be heated for 30 seconds to 3 minutes and 40 seconds; after the reaction is finished, standing and cooling to room temperature, and filtering aproduct by using filter paper and a microfiltration membrane (0.22 [mu]M) to remove macromolecular particles; and centrifuging to obtain a dark brown solution, and freeze-drying the solution to obtain dried brown powder. The method is simple in preparation process and low in preparation condition requirement, and does not need complicated sample pretreatment. The carbon quantum dot prepared by the method has the characteristics of stable optical property, good biocompatibility and the like. The prepared green carbon quantum dot is very sensitive to pH, can quickly penetrate through cell membranes to enter cells, and can detect the pH in living cells in real time.

Description

technical field [0001] The invention relates to a luminescent nanometer material, in particular to a fluorescent carbon quantum dot, in particular to a green fluorescent carbon quantum dot for detecting pH in an acidic environment, a preparation method and application thereof. Background technique [0002] Intracellular pH is an important parameter affecting the life process of organisms. It is closely related to cell proliferation and apoptosis, ion transport, cell homeostasis, enzyme activity, drug antibodies, and endocytosis. The latest medical research shows that an appropriate pH is also a key parameter for maintaining normal physiological activities of the human body. Destroying or slightly altering the pH balance within healthy cytoplasm and organelles can cause enormous harm to organisms, and even directly lead to problems such as cancer, cardiopulmonary disease, or neurology. Compared with normal cells, the pH in cancer cells will be significantly increased or decr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B32/15C09K11/65B82Y20/00B82Y40/00G01N21/64
CPCC01B32/15C09K11/65B82Y20/00B82Y40/00G01N21/6428C01P2004/04
Inventor 闫娅楠宋胜梅孟雅婷张慧林郭忠慧双少敏董川
Owner SHANXI UNIV
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