Use of fig young fruit extract for preparing composition for reducing skin collagen loss
A technology of collagen and figs, applied in the field of plant extracts, can solve the problems of limited effect, enzyme decomposition, and the inability of collagen to penetrate the stratum corneum of the skin to reach the dermis where it acts
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Embodiment 1
[0026] Preparation of fig young fruit extract
[0027] Figs, also known as yingriguo or milk berries, are deciduous shrubs or small trees of Moraceae, native to western Asia and the Mediterranean coast, and are now cultivated in Taiwan, China. The fruit of the plant is rich in dietary fiber and substances beneficial to human health such as calcium, copper, magnesium, manganese, and potassium. Generally, fig fruit can be divided into young fruit and mature fruit according to the developmental stage. Young fruit refers to the fruit whose surface is green and hairy after the fruit is formed; mature fruit refers to the fruit whose surface turns red in color, the hairs are almost removed, and the volume increases sharply.
Embodiment 2
[0031] Young fig fruit extract inhibits collagen glycation
[0032]In order to investigate the effect of the fig young fruit extract of the present invention on collagen glycation, anti-glycation assay was used to measure the fig young fruit extract (10 mg / mL) described in Example 1 or the ripe fig prepared by the method similar to Example 1. Inhibitory effect of fruit extract (10 mg / mL) on the glycation reaction of porcine collagen. Briefly, a 60 mg / mL collagen solution (containing 0.06% sodium azide) and a 1.5 M fructose solution were prepared using 200 mM phosphate buffer (pH 7.4). For the collagen glycation reaction, the mixture of 0.2 mL collagen solution and 0.2 mL fructose solution was mixed with 0.2 mL of fig young fruit or ripe fruit extract sample or deionized water (blank control group), and reacted at 50 °C for 24 hours. After 2 hours, additional aminoguanidine (AG, purchased from Sigma) was added to stop the saccharification reaction. Use a spectrofluorometer (s...
Embodiment 3
[0037] Young Fig Fruit Extract Promotes Collagen Production
[0038] In order to examine the effect of the young fig fruit extract of the present invention on the collagen production of skin cells, in this example, the collagen production test was used to determine the human dermal fibroblasts CCD-966SK treated with the young fig fruit extract described in Example 1. , the change in its collagen production. Briefly, CCD-966SK cells were divided into 2 × 10 4 Cells / well were seeded in 24-well plates, each well containing 500 μL of cell culture medium. After culturing the cells for 24 hours at 37°C, the cell culture medium was removed and each well was treated as follows: (a) 500 μL of 1 mg / mL fig young fruit extract and 500 μL of FBS-free cell culture medium were applied; ( b) 500 μL of 1 mg / mL fig ripe fruit extract and 500 μL of FBS-free cell culture medium; (c) 500 μL of FBS-free cell culture medium (blank control group). After the aforementioned three groups of cells wer...
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