Enteromorpha prolifera polysaccharide with immunomodulatory activity and preparation method of enteromorpha prolifera polysaccharide

Abstract

The invention relates to a preparation method of enteromorpha prolifera polysaccharide PWEP-1 and an immunomodulatory activity effect of the enteromorpha prolifera polysaccharide PWEP-1. The enteromorpha prolifera polysaccharide disclosed by the invention is extracted by a water extraction and alcohol precipitation method and is separated and purified by an ion exchange column and gel column chromatography; the distribution range of the enteromorpha prolifera polysaccharide is 1000-5000Da; and the enteromorpha prolifera polysaccharide is mainly composed of 87.42% of mannose, 7.10% of rhamnose,1.67% of galacturonic acid, 2.67% of galactose and 1.13% of xylose. In-vitro experiment evaluation finds that PWEP-1 can improve the phagocytic ability of RAW264.7 and promote secretion of cell factors such as NO, TNF-alpha and IL-1 beta. PWEP-1 can serve as an immunomodulator to be added into functional food; and therefore, a theoretical basis is provided for research and development of novel enteromorpha prolifera products; and the edible and commercial value of enteromorpha prolifera is improved.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an enteromorpha polysaccharide with immunoregulatory activity extracted by alkaline method and a preparation method thereof. Background technique [0002] Enteromorpha Prolifera (Enteromorpha Prolifera) is a green algae, commonly known as moss strips, seaweed, Chlorophyta, Ulva order, Ulvaceae plants. It is widely distributed in the intertidal zone of the world, especially in China, the Baltic Sea and Chile, and more widely distributed in the east coast of my country. It is recorded in my country's medical classics that Enteromorpha has been eaten and used medicinally by coastal residents of my country since ancient times. In recent years, due to the large-scale reproduction of Enteromorpha, the ecological balance of the marine environment has been destroyed, and even serious "green tide" hazards have been caused. Therefore, how to make better use of this marine resource and turn wast...

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Problems solved by technology

[0004] Chinese patent CN108752495A reports a method for preparing enteromorpha alkali-soluble polysaccharides by enzymolysis and lye extraction, but this method does not check the monosaccharide composition, glycosidic bond type and antitumor activity of the prepared enteromorpha polysaccharides. Research

At present, there is no research report on the immunomodulatory activity of Enteromorpha polysaccharides. This invention is the first to study a low-molecular-weight polysaccharide extracted by alkaline method with immunomodulatory activity, which can provide a certain theoretical basis for the development and application of Enteromorpha polysaccharides.

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