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Chitosan-based nerve fiber membrane, preparation method, nerve guide and application

A nerve fiber and nerve conduit technology, applied in the field of nerve tissue engineering materials, can solve the problems of poor mechanical properties, easy irritation of degraded products, unfavorable local inflammation of the body, etc., and achieve good mechanical properties, favorable for cell adhesion, and suitable for biodegradation sexual effect

Active Publication Date: 2022-05-13
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at problems such as poor mechanical properties and degradation products that easily stimulate the body to cause local inflammation and are not conducive to nerve repair in the existing nerve guides, the invention provides a chitosan-based nerve fiber membrane and a preparation method thereof

Method used

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  • Chitosan-based nerve fiber membrane, preparation method, nerve guide and application
  • Chitosan-based nerve fiber membrane, preparation method, nerve guide and application
  • Chitosan-based nerve fiber membrane, preparation method, nerve guide and application

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preparation example Construction

[0070] The preparation method of described chitosan-based nerve fiber membrane comprises the following steps:

[0071] (1) Preparation of spinning solution: spinning solution includes A solution and B solution, wherein, A solution contains degradable functional materials, carbon nanotubes modified by hydrophilic functional groups, Lycium barbarum polysaccharide, and chitosan and / or chitosan Sugar derivative; B solution contains nerve growth factor and stabilizer.

[0072] The concentration of each component in the A solution and the B solution should ensure that the total content of the composite nanofiber mass content of the orientation arrangement obtained at last is 100% and the content of each component meets the following conditions:

[0073]

[0074] In some embodiments, the concentration of each component of the above-mentioned solution A is respectively:

[0075]

[0076] The solvent used in solution A may be hexafluoroisopropanol (English abbreviation: HFIP). ...

Embodiment 1

[0090] A preparation method of chitosan-based nerve fiber membrane, which comprises the following steps:

[0091] (1). With 1.25g polylactic acid-glycolic acid copolymer (PLGA, wherein the mass ratio of lactic acid monomer and glycolic acid monomer is 75:25), 0.35g chitosan (CS, deacetylation degree is 90%) Dissolve 10 mg of Lycium barbarum polysaccharide (LBP) in 5 mL of hexafluoroisopropanol at the same time, and stir magnetically for 24 hours to fully dissolve PLGA and LBP to form a uniform solution.

[0092] (2). Add 0.1 g carboxy-modified multi-walled carbon nanotubes (MWCNTs, average diameter 15 nm, average length 1 μm) and 100 μL Span 80 to the solution obtained in step (1), and stir magnetically for 8 hours to disperse the MWCNTs uniformly In solution, A solution was obtained.

[0093] (3). Prepare 10 μg / mL Nerve Growth Factor (NGF) aqueous solution, and add bovine serum albumin (BSA) at the same time, control the concentration of BSA to 10 mg / mL to obtain B solution....

Embodiment 2

[0097] A preparation method of chitosan-based nerve fiber membrane, comprising the following steps:

[0098] (1). 0.9g polylactic acid-glycolic acid copolymer (PLGA, wherein the mass ratio of lactic acid monomer and glycolic acid monomer is 85:15), 0.32g chitosan (CS, deacetylation degree is 90%) and 10 mg Lycium barbarum polysaccharide (LBP) were dissolved in 2.5 mL hexafluoroisopropanol at the same time, and magnetically stirred for 24 hours to fully dissolve PLGA and LBP to form a uniform solution.

[0099] (2). Add 0.01 g carboxyl-modified multi-walled carbon nanotubes (MWCNTs, average diameter 10 nm, average length 5 μm) and 50 μL Span 80 to the solution obtained in step (1), and stir magnetically for 8 hours to disperse the MWCNTs uniformly In solution, A solution was obtained.

[0100] (3). Prepare 10 μg / mL Nerve Growth Factor (NGF) aqueous solution, and add bovine serum albumin (BSA) at the same time, control the concentration of BSA to 10 mg / mL to obtain B solution. ...

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Abstract

The invention relates to the technical field of nerve tissue engineering materials, and specifically provides a chitosan-based nerve fiber membrane, a preparation method, a nerve guide and an application. The chitosan-based nerve fiber membrane is formed by a number of composite nanofibers arranged in orientation; each composite nanofiber has a core-shell structure, and its shell layer contains degradable functional materials, carbon nanotubes modified with hydrophilic functional groups, wolfberry Polysaccharide, and chitosan and / or chitosan derivatives, the core layer contains nerve growth factor and stabilizer. Because the chitosan-based nerve fiber membrane of the present invention has good orientation arrangement, good mechanical properties, and high biological responsiveness, the alkalinity of chitosan and / or chitosan derivative hydrolyzate and self-carried amino groups can effectively It neutralizes the weak acidity of the degraded products and avoids the influence of the acidic degraded products on the surrounding environment and tissues, so it can be applied to the nerve repair and regeneration of human and animal bodies.

Description

technical field [0001] The invention belongs to the technical field of nerve tissue engineering materials, and in particular relates to a chitosan-based nerve fiber membrane, a preparation method, a nerve guide and application. Background technique [0002] In modern surgery, tissue repair membranes are often used clinically to repair tissues and organs. This requires tissue repair membranes to have good biocompatibility, certain strength, and facilitate cell adhesion and growth. [0003] In many surgical operations, a common condition is peripheral nerve defect, which often leads to the loss of sensory and motor functions in the corresponding innervated area of ​​the patient. Therefore, the repair and regeneration of peripheral nerve injury has always been a research hotspot in the field of clinical and neuroscience. . [0004] At present, the clinical treatment methods for repair and regeneration after nerve injury mainly include end-to-end suture, autologous or allogene...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/54A61L27/58D04H1/4326D04H1/728D04H1/76
CPCA61L27/58A61L27/54A61L27/20D04H1/4326D04H1/728D04H1/76A61L2400/12A61L2430/32A61L2300/414A61L2300/604C08L5/08
Inventor 赵晓丽刘缘王静潘浩波
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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