Orange fluorescent carbon dot and application thereof in detection of peroxynitrite ions

A fluorescent carbon dot, orange technology, applied in the field of orange fluorescent carbon dots and its preparation, can solve the problems of poor sensitivity/selectivity, complex sample pretreatment, etc., and achieve the effects of environmentally friendly reaction conditions, easy promotion, and simple and effective synthesis methods

Active Publication Date: 2020-09-11
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have their own disadvantages
For example, complex sample pretreatment is required, expensive instrumentation, poor sensitivity / selectivity, and not suitable for intracellular detection, etc.

Method used

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  • Orange fluorescent carbon dot and application thereof in detection of peroxynitrite ions
  • Orange fluorescent carbon dot and application thereof in detection of peroxynitrite ions
  • Orange fluorescent carbon dot and application thereof in detection of peroxynitrite ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation method of carbon dots:

[0030] 1), put 0.03g rhodamine B and 0.05g p-aminophenol in a glass beaker, add ethanol, stir thoroughly and ultrasonically dissolve for 15 minutes;

[0031] 2) Transfer the above solution to a hydrothermal reaction kettle, place it in an oven, and react at 180°C for 10 hours;

[0032] 3) After the reaction stopped, the hydrothermal reaction kettle was left to stand, cooled to room temperature, filtered to remove insoluble matter, rotary steamed to obtain a dark pink solution, and filtered with a 0.22 μm filter membrane to obtain a pure aqueous solution of carbon dots;

[0033] 4) The target product is obtained after the above-mentioned carbon dot solution is freeze-dried.

Embodiment 2

[0035] Fluorescent carbon dots prepared in Example 1 were characterized by fluorescence excitation, emission and ultraviolet absorption spectra (see figure 1 ), the obvious absorption peaks of carbon dots at 221nm and 295nm can be attributed to the aromatic benzene ring sp 2 π-π* transitions and C-N bond n / π* transitions. The optimal excitation and emission wavelengths of CDs are 554nm and 580nm, respectively. Infrared characterization of the carbon dots (such as figure 2 ), the surface contains amino, carboxyl, and hydroxyl groups.

Embodiment 3

[0037] Get 3 mL of fluorescent carbon dot aqueous solution (0.1 mg / mL) prepared in Example 1 and place it in a fluorescent cuvette, then add 11.7 μL of different concentrations of ONOO dropwise to the fluorescent cup - (30-1070nM), mix well, and scan the emission spectrum (λ ex =554nm,λ em =580nm), according to ONOO - The relationship between the concentration and the relative fluorescence intensity change value was calculated for the carbon dots on ONOO - The detection range and detection limit (see image 3 ).

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Abstract

The invention discloses an orange fluorescent carbon dot and application thereof in detection of peroxynitrite ions. The carbon dot (CDs) is prepared from rhodamine B and p-aminophenol as raw materials through a one-step hydrothermal synthesis method; the carbon dot is a zero-dimensional carbon nanomaterial and can emit bright orange fluorescence. The preparation method is simple and convenient, and the prepared carbon dots are good in water solubility and stable in fluorescence property. The peroxynitrite ions can be rapidly and sensitively detected by taking the carbon dots as a fluorescentprobe. When peroxynitrite ions exist, fluorescence of the carbon dots is effectively quenched by peroxynitrite. Meanwhile, the color of the CDs solution is changed into blue black from pink. Comparedwith a pure fluorescence analysis method, the analysis method for detecting the ONOO<-> has the advantages that a dual-mode output method has more advantages in practical application, and it is indicated that the analysis method has potential application prospects in discussion of the effect of the ONOO <-> in cytobiology.

Description

technical field [0001] The invention relates to the detection of carbon nanomaterials and peroxynitrite ions, in particular to an orange fluorescent carbon dot and a preparation method thereof, and the use of the carbon dots in fluorescence and colorimetric dual-mode detection of peroxynitrite ions. Background technique [0002] Peroxynitrite (ONOO - ) is produced by nitric oxide (NO) and superoxide radicals (O 2 . - ) Diffusion-controlled coupling self-reaction generation. Studies have shown that ONOO - It exists in the protonation equilibrium in the form of its acid (ONOOH, pKa=6.8), it can quickly become nitrate. Because of its short lifespan, relatively low steady-state concentration, and multiple in vivo target properties, it has been described as a "stealth" biooxidant. Due to its strong oxidizing properties and good nucleophilicity, ONOO - Can react with different biomolecules, moreover, ONOO - It is believed to be involved in the development of various disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00C01B32/15G01N21/64
CPCC09K11/65B82Y20/00B82Y40/00C01B32/15G01N21/643G01N2021/6432
Inventor 郭建花路雯婧李明璐张慧林焦媛双少敏董川
Owner SHANXI UNIV
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