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Application of myrobalan extract in preparation of malassezia inhibitor

A Malassezia, extract technology, applied in the field of medicine, can solve the problems of drug resistance and side effects

Inactive Publication Date: 2020-09-15
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the problems of drug resistance and side effects of existing clinical anti-Malassezia drugs, it is of great significance to develop a safe and effective Malassezia inhibitor derived from Chinese herbal medicine

Method used

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  • Application of myrobalan extract in preparation of malassezia inhibitor
  • Application of myrobalan extract in preparation of malassezia inhibitor
  • Application of myrobalan extract in preparation of malassezia inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Screening of extracts from traditional Chinese medicines for the activity of Malassezia restrictina

[0045] ①Materials and instruments:

[0046] Three restricted Malassezia strains (Malassezia restricta) were purchased from China Industrial Microorganism Culture Collection Center, strain number: CICC 33081.

[0047] ATCC medium: 2693 modified Dixon (mDixon), ingredients: malt extract 36.0g, ox gall powder 20.0g, peptone 6.0g, glycerin 2.0g, oleic acid 2.0g, water 1000ml, Tween-40 10ml to adjust pH to 6.0 , Add 15g of agar to the solid medium.

[0048] Medium was purchased from Haibo Biotechnology Co., Ltd., batch number: 20190401; agar was purchased from Meilun Biotechnology Co., Ltd., batch number: A1230A, CAS: 9002-18-0; Tween 40 was purchased from Shandong Youso Chemical Technology Co., Ltd., CAS: S12702115.

[0049] Electric heating mantle, manufacturer: Haining Huaxing Instrument Factory, model: TC-15; rotary evaporator, manufacturer: Shanghai Ailang...

Embodiment 2

[0067] Example 2: Extraction and preparation of chebula water extract and 95% ethanol extract of chebula and screening of activity against different fungi

[0068] Materials and instruments: Chinese medicine Myrobalan was purchased from Beijing Tongrentang (origin, Yunnan; batch number, 181120; manufacturer, Guangxi Wuzhou Guikang Pharmaceutical Co., Ltd.); 2693 modified Dixon (mDixon) medium (Qingdao Hi-Tech Industrial Park Technology Co., Ltd., batch number: 20190508); XTT reagent (Guangzhou Ruishu Biotechnology Co., Ltd.); dimethyl sulfoxide (DMSO) (Guangzhou Ruishu Biotechnology Co., Ltd.); sodium chloride (Nacl) (Guangzhou Chemical Reagent Co., Ltd. company); RPMI Medium1640 powder (GIBICO, USA, batch number: 1256837); sterile PBS (0.01M) (HyClone, USA, batch number: AXH440260); absolute ethanol (Tianjin Zhiyuan Chemical Reagent Co., Ltd.); Multiskan FC enzyme label Instrument (Thermo Fisher (Shanghai) Instrument Co., Ltd.); constant temperature culture shaker.

[0069] ...

Embodiment 3

[0092] Example 3: Preparation and component analysis of Myrobalan extract

[0093] Materials and instruments: The traditional Chinese medicine Myrobalan was purchased from Beijing Tongrentang (origin, Yunnan; batch number, 181120; manufacturer, Guangxi Wuzhou Guikang Pharmaceutical Co., Ltd.), Waters UPLC-Q / TOF MS liquid chromatography mass spectrometry (Water, USA World Limited).

[0094] 1. Preparation of Myrobalan extract

[0095] Weigh 20g of dry powder of Myrobalan, add water to extract twice by reflux extraction method, the ratio of solid to liquid is 1:10, 1:8 respectively; the extraction time is 2h, 1h respectively; the filtrates are combined twice, passed through a 2000Da organic membrane, and retained For the small molecule part, the extract is concentrated and purified, and concentrated under reduced pressure to obtain the chebula extract.

[0096] 2. Analysis of the main components of Myrobalan extract

[0097] Chromatographic conditions: chromatographic column ...

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Abstract

The invention discloses an application of a myrobalan extract in preparation of a malassezia inhibitor, and further discloses an application of the myrobalan extract in preparation of a medicine for treating and / or preventing malassezia infection-related skin diseases. The skin diseases related to malassezia infection comprise pityriasis versicolor, malassezia folliculitis, seborrheic dermatitis and other diseases. The invention provides the malassezia inhibitor comprising the myrobalan extract and the medicine for treating and / or preventing skin diseases associated with malassezia infection.Researches show that the myrobalan extract and the main components thereof have a good inhibition effect on malassezia, have a good prevention and treatment effect on malassezia infection-related diseases, are safe and free of irritation and side effects, and can be used for preparing related medicines.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically relates to the application of chebula extracts in the preparation of Malassezia inhibitors. Background technique [0002] Malassezia (Malassezia) is a group of common conditional pathogenic bacteria, which widely exist in the skin oil secretion of human beings and warm-blooded animals. It is one of the normal flora of the human body and is in a symbiotic state with the body. The mechanism of Malassezia in the pathogenesis of skin diseases is still controversial. Colonization on human skin can not only cause non-inflammatory skin diseases such as pityriasis versicolor (formerly known as tinea versicolor, commonly known as sweat spots), but also associated with Inflammatory skin diseases such as seborrheic dermatitis, atopic dermatitis, folliculitis and other diseases have a certain correlation. Studies have found that the number of Malassezia bacteria on the scalp of patien...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P31/10A61P17/00A61P17/08A61P17/04A61P29/00A61K8/9789A61Q19/00A61Q17/00A61K131/00
CPCA61K36/185A61K9/0014A61P31/10A61P17/00A61P17/08A61P17/04A61P29/00A61K8/9789A61Q19/00A61Q17/005
Inventor 沈志滨
Owner GUANGDONG PHARMA UNIV
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