Method for detecting histamine by nano-enzyme labeled direct competitive bionic immunoassay
A technology of biomimetic immunity and nanozyme, which is applied in the direction of analytical materials, biological testing, measuring devices, etc., can solve the problems of poor adsorption capacity and elution effect of target substances, easy inactivation of enzymes, and use restrictions, etc., to achieve convenient adsorption and washing The effect of detachment, good pore structure and good stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0040] In one embodiment of the present invention, a nanozyme-labeled antigen preparation method is provided, comprising the steps of:
[0041] Mix chloroplatinic acid solution and polyvinylpyrrolidone in ethanol solution, heat and reflux at 100°C for 3 to 4 hours, after cooling, store the finished product at 4°C in the dark;
[0042] Ammonia water was added to the Pt NPs solution as a catalyst, tetraethyl silicate was added dropwise, and reacted at room temperature for 2-4 hours to obtain Pt@SiO coated with silica. 2 ;
[0043] For the obtained Pt@SiO 2 Perform multiple washes to remove excess tetraethyl silicate, then add 3-aminopropyltriethoxysilane dropwise at room temperature, react for 3-6 hours, after the reaction, wash multiple times to obtain the modified Pt @SiO 2 nanozyme;
[0044] Aminated Pt@SiO 2 Mix the material with glutaraldehyde for 0.5-1h; add histamine solution for labeling, after the reaction, centrifuge and wash the reactant three times to obtain Pt@...
Embodiment 1
[0063] 1. Pt@SiO 2 Synthesis of @HA:
[0064] (1) 1.55mL H 2 PtCl 6 ·6H 2 O aqueous solution (38.6 mM), 6.66 mg polyvinylpyrrolidone PVP and 81 mL ethanol were added to a round bottom flask and mixed. The above mixture was heated to reflux at 100 °C for 3 h to prepare PtNPs. After cooling, store the finished product in a dark place at 4°C.
[0065] (2) Pt NPs modification: Disperse 20 mL of PtNPs in a 50 mL round bottom flask. Add 100 µL of ammonia as a catalyst. Add 200 μL tetraethoxysilane dropwise and keep stirring to disperse. The reactant solution was reacted at room temperature for 2h. Pt@SiO obtained by centrifugation 2 Nanoparticles were washed 3 times with ethanol repeatedly to remove excess tetraethoxysilane and ammonia water. Pt@SiO 2 Nanoparticles were dispersed in 10 mL of ethanol. Then 200 μL of 3-aminopropyltriethoxysilane was added dropwise and reacted for 4 hours. Collected by centrifugation and washed 3 times with ethanol. Finally, 1 mL of gluta...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More