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Microorganism composite microbial inoculum capable of restraining mycelial fungi and application of microorganism composite microbial inoculum

A technology of composite bacterial agents and microbial strains, applied in the direction of microorganisms, methods based on microorganisms, food ingredients as antimicrobial preservation, etc., can solve problems such as feed perishability, food safety, and crop spoilage, and achieve non-perishable safety , Nutritious, aromatic effect

Active Publication Date: 2020-09-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, due to being rich in nutrients such as amino acids and moisture, crop wastes are easily attacked by filamentous fungi, while filamentous fungi such as Penicillium expanses, A. The leaves and fruits of the crops cause corruption of the crops, which in turn causes storage diseases of the crops, and they will produce toxins such as patulin and citrinin, which will remain in the crops and then enter the human body through the food chain, which is potentially harmful) Both are one of the culprits that cause crop spoilage. Therefore, even after sterilization, the feed prepared from crops is easy to spoil during storage.
[0006] In order to solve this problem, people such as Ma Qingsong tried to add preservatives to the feed prepared from crops to prolong its shelf life (see references for details: Ma Qingsong, Wang Weiguo. The impact of different antifungal agents on the storage quality of pellet feed [J]. Feed Industry, 2019,40(9):38-44), however, there are food safety problems in adding a large amount of preservatives, and chemical additives have many restrictions such as high cost, low return and unfavorable environmental protection (specifically Visible references: Yu Zhengrong, Wu Yinchao, Xie Jiayu, etc. Antifungal agent sodium dehydroacetate induced bleeding test in rats [J]. Advances in Veterinary Medicine, 2018,39(1):73-78 and references: Wang Xueyang, Han Shumin, Li Jinku, etc. Application of antifungal agents in feed [J]. Animal Husbandry and Feed Science, 2019, 40(3): 50-52), and insufficient preservative addition will lead to poor antiseptic effect

Method used

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  • Microorganism composite microbial inoculum capable of restraining mycelial fungi and application of microorganism composite microbial inoculum
  • Microorganism composite microbial inoculum capable of restraining mycelial fungi and application of microorganism composite microbial inoculum
  • Microorganism composite microbial inoculum capable of restraining mycelial fungi and application of microorganism composite microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0074] Example 1-1: Screening, identification, cultivation and observation of Bifidobacterium adolescentis CCFM1108

[0075] 1. Screening

[0076] Take 1g of feces samples from healthy adults in Wuxi area, dilute them with normal saline, spread them on mMRS solid medium, culture them in an anaerobic environment at 37°C for 72 hours, observe and record the colony morphology; pick colonies in mMRS Streak on the solid medium, carry out purification culture at 37°C in an anaerobic environment, and obtain a purified single colony; pick a single colony and streak on the mMRS solid medium, culture anaerobically at 37°C for 48 hours, and test the obtained colony Gram staining (Gram staining method refers to the textbook "Industrial Microbial Breeding" author: Zhuge Jian), record the colony morphology, and investigate the physiological and biochemical characteristics of the strain according to the textbook "Common Bacterial System Identification Manual" (Author: Dong Xiuzhu) , to reta...

Embodiment 1-2

[0091] Example 1-2: Effect of Bifidobacterium adolescentis CCFM1108 and its fermentation supernatant on the germination rate of filamentous fungal spores

[0092] 1. Effect of Bifidobacterium adolescentis CCFM1108 on the germination rate of filamentous fungal spores (double-layer plate-growth inhibition method)

[0093] Pick the single bacterium colony of Bifidobacterium adolescentis CCFM1108 screened in Example 1 and streak it on the mMRS solid medium, and cultivate it at 37° C. for 48 hours in an anaerobic environment to obtain a single bacterium colony; pick a single bacterium colony and inoculate it in the mMRS liquid medium, Cultivate at 37° C. for 48 hours under anaerobic environment, repeat this operation 3 times, and obtain the bacterial liquid cultivated to the third generation.

[0094] Use an inoculation loop to inoculate the Penicillium extensa liquid in the ampoule tube on the PDA medium, and incubate at 28°C for 7 days to obtain mycelia and spores; pick the spore...

Embodiment 1-3

[0109] Example 1-3: Effect of Bifidobacterium adolescentis CCFM1108 fermentation supernatant on the growth of Penicillium expanse mycelium

[0110] Mix the mMRS liquid medium with the PDA medium at a volume ratio of 1:9, 1.5:8.5, 2:8, 2.5:7.5, 3:7 (mMRS liquid medium: PDA medium) to obtain mMRS liquid medium Concentration is respectively the control group mixed solution of 10,15,20,25,30% (v / v); The fermentation supernatant that embodiment 2 obtains is respectively 1:9,1.5:8.5,2:8,2.5: The volume ratio of 7.5, 3:7 (fermentation supernatant: PDA medium) was mixed with PDA medium to obtain experimental groups with fermentation supernatant concentrations of 10, 15, 20, 25, 30% (v / v) respectively Mixed solution; Pour the mixed solution of the control group and the experimental group into the plate respectively; add 10 μL of the Penicillium expanse spore suspension obtained in Example 2 dropwise to the center of the plate, and cultivate it at 28°C for 6 days. The diameter of mycel...

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Abstract

The invention discloses a microorganism composite microbial inoculum capable of restraining mycelial fungi and an application of the microorganism composite microbial inoculum, belongs to the technical fields of biology and fermentation, and provides the microorganism composite microbial inoculum which can effectively restrain mycelial fungi of penicillium expansum, aspergillus niger, penicilliumroqueforti, penicillium digitatum and the like. The microorganism composite microbial inoculum consists of bifidobacterium adolescentis of which the preservation No. is GDMCC No.60925 and lactobacillus plantarum of which the preservation No. is CGMCC No.5494. A fermented feed prepared from the microorganism composite microbial inoculum is rich in nutrients, fragrant in odour, not liable to corruptand high in safety, and specific reflection lies in that the content of mould in the fermented feed prepared from the microorganism composite microbial inoculum is 0CFU / g, and after the fermented feed is placed at 30 DEG C for 15d, the content of the mould in the fermented feed prepared from the microorganism composite microbial inoculum is 0CFU / g.

Description

technical field [0001] The invention relates to a microbial compound bacterial agent capable of inhibiting filamentous fungi and an application thereof, belonging to the field of biotechnology. Background technique [0002] Apple is the largest fruit in my country and one of the most popular crops, with a wide planting range, large area and high yield. my country's apples are mostly used to produce apple juice concentrate, and a large amount of apple pomace will be discharged during the production of apple juice concentrate. According to statistics, in 2011 alone, the output of apple pomace in my country reached 4 million tons. The effective utilization and disposal of these apple pomace has become a major problem in my country's crop processing industry. [0003] In addition to apple pomace, fruit waste such as blueberry pomace left over from blueberry juice production, mulberry pomace left over from mulberry juice production, grape pomace left over from grape juice produ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K10/12A23K10/30A23K10/37A23L3/3571C12R1/01C12R1/25
CPCC12N1/20A23K10/18A23K10/12A23K10/30A23K10/37A23L3/3571A23V2002/00A23V2400/513A23V2400/169A23V2200/10Y02P60/87
Inventor 于雷雷陈卫田丰伟乔楠桢翟齐啸闫博文赵建新张灏
Owner JIANGNAN UNIV
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