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Biochemical ultramicro-detection process by means of multifunctional biochemical enzyme label analyzer

A detection technology, a multi-functional technology, applied in the direction of biochemical equipment and methods, biological testing, microbial determination/inspection, etc., can solve the problem of no biochemical ultra-trace and rapid colorimetric analysis technology methods, sampling, detection instruments, detection methods It does not pass the test and affects the accuracy of the measurement results, so as to achieve the effect of saving blood, reducing the cost of reagent consumption, and improving the speed of colorimetric testing

Inactive Publication Date: 2003-08-06
吴斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most domestic biochemical tests still use venous blood drawing, constant method, and manual colorimetry to read the absorbance and then calculate the content; while the advanced automatic biochemical analyzers produced abroad can quantify the test samples without manual calculation. Results can be obtained, but it is also inseparable from venous blood drawing, and there is still a shortage of waste of reagents when the sample volume is small.
In order to avoid the patient's venous blood drawing, it is necessary to require as few samples as possible for each measurement without affecting the accuracy of the results. This is the current difficulty in biochemical testing at home and abroad.
[0004] For semi-automatic biochemical analyzers, in the detection of large batches of samples, there are shortcomings that directly affect the accuracy of the measurement results due to the time difference between the standard product and the tested sample before and after the test, especially the slow constant method manual colorimetric test
[0005] In addition to the above factors, there are also shortcomings in sampling, testing instruments, and testing methods, and more importantly, there is no complete set of supporting instruments and corresponding technical methods suitable for biochemical ultra-trace and rapid colorimetric analysis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Determination of glucose oxidase method (end point method)

[0029] 1. Peripheral blood collection:

[0030] (1) The blood collection site is suitable for adults to use the ring finger of the left hand, and infants under half a year old usually use the thumb or heel to collect blood.

[0031] (2) Gently massage the blood collection site to make it congest naturally, disinfect the local skin with 75% ethanol cotton ball, and let it dry.

[0032] (3) Squeeze the lancet site tightly and puncture it with a sterile blood collection needle to take blood. The action should be quick, and the depth should be about 2-3mm. It is advisable to squeeze the blood out slightly.

[0033] (4) Take a polyethylene plastic tube with an inner diameter of 2-3 mm and a length of 5-7 mm (micro-volume blood collection plastic tube) with the upper end in contact with the blood drop, lower the lower end, squeeze the blood while gently squeeze it, collect about 1 drop of blood and burn...

Embodiment 2

[0057] Serum Cholesterol Determination - Enzymatic Colorimetric Method

[0058] 1. Peripheral blood collection is the same as in Example 1.

[0059] 2. Principle: After cholesterol esterase hydrolyzes cholesterol ester, cholesterol is oxidized by cholesterol oxidase to produce H 2 o 2 , and then determined by Trinder reaction, so as to find its content.

[0060] 3. Reagents: produced by Beijing Beihua Fine Chemicals Co., Ltd.

[0061] Kit composition:

[0062] Cholesterol lipase 400U / L Cholesterol oxidase 500U / L

[0063] Peroxidase 200U / L 4-aminoantipyridine 1mmol / L

[0064] Phenol 10mmol / L Phosphate buffer (PH1.0±0.1)

[0065] Cholesterol standard solution 5.17mmol / L (200mg / dl)

[0066] Dissolve according to the packaging specifications before application, and make an enzyme application solution.

[0067] 4. Adding samples: Take a clean 8×12 standard 96 microwell plate and add samples according to the table below:

[0068] Addit...

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PUM

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Abstract

In the present invention, millipore plate is used to replace test tube and colorimetric cups and the test operation is performed in millipores directly. By means of micro blood sampling tube of polyethylene plastic in different size and quantitative transfer device, the biochemical test uses no venous blood drawing, less reagent amount and micro sample. Micro blood is made to react with corresponding reagent for certain period and by combination with biochemical enzyme label analyzer, may be constituted one perfect fast micro detection system capable of detecting, analyzing and printing test report automatially.

Description

technical field [0001] The invention belongs to the field of medical biochemical testing. Specifically, it is a method for testing biochemical ultra-micro-quantity rapid detection technology with a biochemical enzyme label multifunctional analyzer. Background technique [0002] Biochemical testing is an important part of medical testing. The development of biochemical analysis in the direction of micro, fast, accurate and convenient is the inevitable trend of current domestic and foreign medical testing. [0003] At present, most domestic biochemical tests still use venous blood drawing, constant method, and manual colorimetry to read the absorbance and then calculate the content; while the advanced automatic biochemical analyzers produced abroad can quantify the test samples without manual calculation. Can draw the result, but can't get rid of venous blood drawing equally, there is also the deficiency of starting up and wasting reagents under the situation that sample vol...

Claims

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Application Information

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IPC IPC(8): C12Q1/30G01N21/27G01N33/50
Inventor 吴斌张红宁梁立森韩明伦
Owner 吴斌