Bilirubin derivative-based diagnostic and therapeutic ultrasound contrast agent
A technology of bilirubin and derivatives, applied in the field of ultrasound contrast agents, can solve the problem of low image quality
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0093] Embodiment 1: Preparation of the ultrasound contrast agent based on pegylated bilirubin of the present invention
[0094] 1-1. Preparation of bilirubin derivatives (pegylated bilirubin, Pegylated bilirubin)
[0095] The present inventors prepared amphiphilic derivatives of bilirubin in which a hydrophilic molecule was bound to bilirubin prior to preparation of a bilirubin-based ultrasound contrast agent. As the hydrophilic molecule, polyethylene glycol (polyethyleneglycol) was used.
[0096] Specifically, first dissolve bilirubin in dimethylsulfoxide (DMSO), and then add an appropriate amount of EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide) to activate the carboxyl groups present in bilirubin , so as to induce the desired reaction, and react at room temperature for 10 minutes. Thereafter, polyethylene glycol having an amine group at the end is added and reacted for a period of time to synthesize a bilirubin derivative (Pegylated bilirubin) in which the carboxyl ...
Embodiment 2
[0100] Example 2: Phantom Imaging of Pegylated Bilirubin-Based Ultrasound Contrast Agents of the Invention
[0101] Ultrasound phantom imaging was acquired using a Vevo770 (High-Resolution Micro-Imaging System, Visualsonics, Toronto, Canada) equipped with an ultrasound device probe for mice, RMV 706 probe. In order to simulate in vivo conditions for ultrasound imaging, the present inventors used an agarose-gel phantom made by embedding 500 μL unit microcentrifuge tubes in 3% (w / v) produced in agarose gel.
[0102] First, 300 μL of PEGylated bilirubin-based contrast agent samples (PFP 0, 2.5, 5, 10% v / v) of the present invention using perfluoropentane (perfluoropentane, PFP) as the hydrophobic gaseous core Placed in an agarose-gel phantom, and images were acquired by 40 MHz ultrasound. The change in ultrasonic intensity of each sample (PFP respectively 0, 2.5, 5, 10% v / v) was measured over a period of 180 minutes, and as a normalization process, the water control group wa...
Embodiment 3
[0106] Example 3: Characteristics of the Pegylated Bilirubin-Based Ultrasound Contrast Agent of the Invention
[0107] 3-1. Microscopic morphology
[0108] Microscopic morphology of fine particles was performed by negative staining with uranium acetate and transmission electron microscopy (Tecnai G2 F30, Eindhoven, Netherlands) ( Figure 6 ) and the optical microscope under the coverslip ( Figure 7 ) to observe.
[0109] Figure 6 This is a picture of a pegylated bilirubin-based ultrasound contrast agent observed with a transmission electron microscope (TEM, transmission electron microscopy). Figure 6 Micron-sized air bubble particles constituting the ultrasound contrast agent of the present invention are shown. Figure 7 is a pegylated bilirubin-based ultrasound contrast agent for observation by light microscopy.
[0110] In addition, in order to calculate the number of air bubbles contained per volume of the contrast agent, the pegylated bilirubin-based contrast agent o...
PUM
Property | Measurement | Unit |
---|---|---|
molecular weight | aaaaa | aaaaa |
size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com