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Preparation method and application of NiCo2O4 electrochemiluminescence sensor based on oxygen-enriched vacancy

An electrochemistry and sensor technology, applied in chemiluminescence/bioluminescence, electrochemical variables of materials, analysis by chemical reaction of materials, etc. Chemiluminescence reaction, good reproducibility, low cost effect

Active Publication Date: 2020-10-13
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nevertheless, the ABEI-dissolved oxygen system also has certain limitations, and its luminescence performance is not ideal, which also limits its application in electrochemiluminescence sensors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1. A kind of NiCo based on oxygen-rich vacancies 2 o 4 The preparation method and application of the electrochemiluminescence sensor is characterized in that it comprises the following steps:

[0027] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;

[0028] (2) Drop-coat 6 μL of ABEI@Pd / NiCo at a concentration of 2 mg / mL on the surface of the glassy carbon electrode 2 o 4 The solution is the sensing substrate, which is placed at 37 °C to dry;

[0029] (3) Add 6 μL of CYFRA 21-1 antibody solution at a concentration of 100 μg / mL dropwise, rinse the electrode surface with pH 7.4 phosphate buffer solution PBS, and place it to dry at 4 °C;

[0030] (4) Add 3 μL bovine serum albumin solution with a mass fraction of 1% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 7.4 phosphate buff...

Embodiment 2

[0032] Example 2. A NiCo based on oxygen-rich vacancies 2 o 4 The preparation method and application of the electrochemiluminescence sensor is characterized in that it comprises the following steps:

[0033] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;

[0034] (2) Drop-coat 6 μL of ABEI@Pd / NiCo at a concentration of 3 mg / mL on the surface of the glassy carbon electrode 2 o 4 The solution is the sensing substrate, which is placed at 37 °C to dry;

[0035] (3) Add 6 μL of CYFRA 21-1 antibody solution at a concentration of 100 μg / mL dropwise, rinse the electrode surface with pH 7.4 phosphate buffer solution PBS, and place it to dry at 4 °C;

[0036] (4) Add 3 μL bovine serum albumin solution with a mass fraction of 2% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 7.4 phosphate buffer soluti...

Embodiment 3

[0038] Example 3. A NiCo based on oxygen-rich vacancies 2 o 4 The preparation method and application of the electrochemiluminescence sensor is characterized in that it comprises the following steps:

[0039] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;

[0040] (2) Drop-coat 6 μL of ABEI@Pd / NiCo at a concentration of 4 mg / mL on the surface of the glassy carbon electrode 2 o 4 The solution is the sensing substrate, which is placed at 37 °C to dry;

[0041] (3) Add 6 μL of CYFRA 21-1 antibody solution at a concentration of 100 μg / mL dropwise, rinse the electrode surface with pH 7.4 phosphate buffer solution PBS, and place it to dry at 4 °C;

[0042] (4) Add 3 μL bovine serum albumin solution with a mass fraction of 3% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 7.4 phosphate buffer soluti...

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PUM

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Abstract

The invention relates to a preparation method and application of a NiCo2O4 electrochemiluminescence sensor based on oxygen-enriched vacancy, and belongs to the field of novel nano materials and the technical field of biosensing. According to the invention, N-(4-aminobutyl)-N-ethyl isoluminol (ABEI) is used as a reducing agent to prepare palladium functionalized oxygen-enriched vacancy NiCo2O4 (ABEI@Pd / NiCo2O4) as a sensing substrate; an immunosensor is developed and applied to actual sample detection of a non-small cell lung cancer disease marker CYFRA 21-1. Oxygen-enriched vacancy NiCo2O4 isprepared through a sodium borohydride normal-temperature reduction method; the high-concentration oxygen vacancy can improve the NiCo2O4 electronic structure; electron mobility is enhanced, and the oxygen-enriched vacancy NiCo2O4 has more excellent electrochemical activity; dissolved oxygen O2 in the electrolyte can be efficiently catalyzed to be converted into superoxide anion free radicals O2 <.->, so that the electrochemical luminescence reaction between ABEI and O2 <.-> is enhanced, and efficient and stable signal output is achieved; the detection limit of the sensor is 20 pg / mL, the linear range is 50 pg / mL-50 ng / mL; and the sensor has obvious potential application value in early diagnosis of non-small cell lung cancer.

Description

technical field [0001] The invention belongs to the field of novel nanometer materials and the technical field of biosensing. Background technique [0002] CYFRA 21-1 is a soluble fragment of cytokeratin 19, which is considered to be a tumor marker mainly used in the detection of lung cancer, especially for the diagnosis of non-small cell lung cancer. If there are unclear circular shadows in the lungs and the serum CYFRA21-1 concentration is higher than 30 ng / mL, the possibility of primary bronchial lung cancer is very high, and the positive detection rate of various types of non-small cell lung cancer is 70% to 85%. The serum concentration of CYFRA21-1 is positively correlated with the clinical stage of the tumor, and it can also be used as an effective indicator for tracking early recurrence of lung cancer after surgery, radiotherapy and chemotherapy. A high serum concentration of CYFRA 21-1 indicates that the disease is in an advanced stage and the prognosis is poor. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327G01N21/76G01N33/574
CPCG01N27/48G01N27/3277G01N27/3278G01N21/76G01N33/57423G01N33/57484
Inventor 魏琴杨磊杜宇杨兴龙刘蕾任祥张诺胡丽华
Owner UNIV OF JINAN