Urine nucleic acid maker and detection reagent kit for assisting in early screening of prostatic cancer

A technology for prostate cancer and detection reagents, applied in the fields of molecular biology and medical diagnostics, can solve the problems of interfering with the relative expression level of nucleic acid markers, subtle deviations in the working efficiency of detection systems, and inability to eliminate deviations in detection equipment, and achieve good clinical applications. Prospect, ideal working performance, effect of guaranteed reliability
CN111778331AActive Publication Date: 2020-10-16诺迦(杭州)生物工程有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
诺迦(杭州)生物工程有限公司
Publication Date
2020-10-16

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Abstract

The invention discloses a urine nucleic acid maker and detection reagent kit for assisting in early screening of prostatic cancer. The nucleic acid marker comprises three prostatic cancer related genes of a T2E fusion gene, a PCA3 gene and an MMP-2 gene, and two internal reference genes of a PSA gene and an SPDEF gene. A multiple fluorescence reverse transcription polymerase chain reaction (RT-PCR) detection reagent comprising the five nucleic acid markers can be used for assisting in screening of prostatic cancer, an area under curve (AUC) of a testee operating characteristic (ROC) curve canachieve 0.81, and sensitivity predicted value and negative predicted value are respectively as high as 91.6% and 93.3%. The detection reagent kit is simple to operate, and quantitative detection of the nucleic acid markers and screening of the prostatic cancer can be completed in one pipe of the reagent. Unnecessary puncturing inspection of prostate can be notably reduced, and the reagent kit hasfavorable clinical application prospects.
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Description

Technical field

[0001] The invention relates to the fields of molecular biology and medical diagnostics, in particular to a urine nucleic acid marker and a detection kit for assisting in the early screening of prostate cancer. Background technique

[0002] With the aging of our country's population structure and the changes in people's living habits, prostate cancer has quietly become one of the common tumors that seriously threaten the health of men in our country. The survey results in 2018 showed that the total number of prostate cancer patients in my country was nearly 145,000, and the compound growth rate in the last five years was nearly 10% higher. It is the tumor type with the fastest growing incidence. If prostate cancer is detected early and treated in time, the five-year survival rate can reach more than 95%, while the five-year survival rate of advanced prostate cancer that has metastasized is less than 30%. Only one-quarter to one-third of newly diagnosed patients i...

Claims

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