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Preparation method of layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nano composite

A nanocomposite, hydroxide technology, applied in the field of medical materials, can solve the problem of no anti-cancer effect, and achieve the effect of improving production efficiency and utilization rate

Active Publication Date: 2020-10-30
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, LDH nanosheets have no anti-cancer effect by themselves, and need to cooperate with other drugs or materials to exert their effect

Method used

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  • Preparation method of layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nano composite
  • Preparation method of layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nano composite
  • Preparation method of layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nano composite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) 16.0mmol sodium hydroxide was dissolved in 37.5mL deionized water to obtain sodium hydroxide solution;

[0037] (2) 1.7mmol aluminum nitrate and 5.1mmol magnesium nitrate were dissolved in 12.5mL deionized water to obtain a metal salt solution;

[0038] (3) Under a nitrogen atmosphere, slowly drop sodium hydroxide solution into the metal salt solution, stir vigorously at room temperature for 1 hour, then centrifuge at 12,000 rpm for 15 minutes, wash with water three times, and collect the layered double hydroxide Nanosheet crude product;

[0039] (4) The crude product of layered double hydroxide nanosheets was dispersed in 40 mL of deionized water, placed in a hydrothermal reaction kettle, and placed in an oven at 120° C. for 8 hours to obtain layered double hydroxide nanosheets.

[0040] (5) 7.25 mg of the prepared layered double hydroxide nanosheets, 6.8 mg of copper chloride, and 27 mg of polyvinylpyrrolidone were dissolved in 9 mL of deionized water to obtain a...

Embodiment 2

[0044] Take 2 mg of the layered double hydroxide-copper sulfide quantum dot nanocomposite prepared in Example 1, disperse it in 2 mL of water and place it in a cuvette. cm 2 The 808nm near-infrared laser irradiates the solution, and the temperature of the solution changes with time. figure 2 It can be seen that the obtained nanocomposite undergoes obvious photothermal conversion under illumination, and the temperature of the solution continues to rise as the illumination time prolongs.

Embodiment 3

[0046] The layered double hydroxide-copper sulfide quantum dot nanocomposite prepared in Example 1 was used for intracellular lysosome localization experiments.

[0047] (1) The layered double hydroxide-copper sulfide quantum dot nanocomposite prepared in Example 1 was stirred in an aqueous solution of an equal amount of green fluorescent marker (FITC) for 4 hours. Human breast cancer cells 4T1 were inoculated in a 12-well cell culture plate, 1×10 per well 5 For each cell, add 1 mL of DMEM medium to the culture medium in each well. After the cells were adhered to the wall for 24 hours, the original medium was taken out, and 500 μL of nanocomplexes labeled with FITC containing 10 μg / mL were added to the experimental group, and the culture was continued for 4 hours;

[0048](2) Aspirate the supernatant in the well plate, wash the cells with phosphate buffer solution, and mark the cell lysosomes with the lysosome probe LysoTracker Rad, observe and take pictures under the laser co...

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PUM

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Abstract

The invention discloses a preparation method of a layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nano composite, which comprises the following steps: growing copper sulfide quantum dots on a layered double hydroxide nanosheet by using an in-situ growth method, and independently walking CuS on LDH without agglomeration by using a polymer confinement effect. On one hand,the prepared layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nano composite has the photothermal property, near-infrared light can be irradiated and then converted into heat energy, and the tumor photothermal treatment effect is achieved; and on the other hand, the formed metal-semiconductor heterostructure is beneficial to separation of electrons and holes of copper sulfide under near-infrared irradiation, so that active oxygen with biotoxicity is generated in tumor cells more efficiently under catalysis, and the tumor cells are killed.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a method for preparing a layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nanocomposite. Background technique [0002] With the development of tumor therapy, nanomaterials have superior morphological, physicochemical, and functional properties, and various therapeutic strategies have undergone extensive changes. Various active targeting strategies targeting specific organelles have attracted increasing attention in recent years. In particular, lysosomes are the first organelles to transport nanomaterials after endocytosis. Compared with normal cells, lysosomes in cancer cells are overdeveloped, large in size, high in number, and high in cathepsin activity, showing the potential of lysosomes as targeted organelles to enhance selectivity and significant efficacy in tumor therapy. [0003] Copper sulfide (CuS) has been widely studie...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K49/22A61P35/00B82Y30/00B82Y40/00C01F7/00C01G3/12
CPCA61K41/0052A61K41/0057A61K49/222A61P35/00C01F7/00C01G3/12B82Y30/00B82Y40/00C01P2004/04C01P2004/20C01P2004/10A61K2300/00
Inventor 陈爱政刘晨光王士斌
Owner HUAQIAO UNIVERSITY
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