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Bacillus firmus as well as preparation method and application thereof

A technology of Bacillus firmus and Bacillus, applied in the field of microbial inoculum products, to achieve the effect of reducing agricultural production costs, reducing the use of pesticides, and not producing resistance

Active Publication Date: 2020-11-03
天津开发区坤禾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, there is no effective microbial agent to control sunflower sclerotinia

Method used

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  • Bacillus firmus as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of MES80101 microbial agent is carried out according to the following steps:

[0031] ①First-level seed culture: The strain MES80101 activated on the test tube slant was cultured in an Anbei bottle, and the Anbei bottle medium was a nutrient agar medium. The medium formula is: peptone 8-13g, beef extract 2-5g, sodium chloride 3-5g, agar 19.5g, ultrapure water 1000ml, and the pH value is 7.0-7.5. The culture conditions are: temperature 25°C-30°C, culture for 24h.

[0032] ②Fermentation: 20L liquid Bacillus firmus fermentation medium is installed in a 30m3 fermenter, sterilized by moist heat at 115°C-122°C for 35-40min, then cooled to 35°C, scraping the first-grade seed lawn into 100mL sterile water at room temperature, After mixing, inoculate in the Bacillus firmus liquid fermentation medium, control the tank pressure at 0.02MPa-0.07MPa, and cultivate at 35°C for 26h; the raw materials and dosage of the medium used for fermentation are: glucose 1%, corns...

Embodiment 2

[0038] Antibacterial test of Bacillus firmus MES80101 on sunflower sclerotinia:

[0039] (1) Test time and place: In November 2019, it will be carried out in the laboratory of the company's R&D center.

[0040] (2) Test method:

[0041] (1) The source of the tested bacteria

[0042] The sunflower sclerotiorum pathogen comes from the sunflower farmland in Chifeng City, Inner Mongolia. It was cultivated, isolated, and purified by the company's R&D personnel. It was finally identified as Sclerotias clerotiorum (Lib.) deBary by 16S rDNA, and it was identified as strong pathogenicity by pathogenicity testing .

[0043] Bacillus firmus is prepared from the strain described in Example 1 through fermentation and powder spraying.

[0044] (2) Plate antagonism experiment

[0045] Prepare a PDA solid plate, pick a sunflower sclerotinia sclerotia sclerotia with a sterile inoculation needle and place it in the middle of the petri dish, burn the inoculation needle red, dip a small amount ...

Embodiment 3

[0049] Pot control effect of Bacillus firmus MES80101 on sunflower sclerotinia:

[0050] (1) Test drug

[0051] (1) Microbial agent

[0052] The MES80101 solid preparation prepared in Example 1 was diluted with water 10 times, 20 times, 30 times and 50 times respectively;

[0053] (2) Chemical agents

[0054] 40% Sclerotia net wettable powder 800-1200 times liquid (Shandong University of Science and Technology Entrepreneurial Biology Co., Ltd.).

[0055] (3) Blank control

[0056] Set clean water treatment as blank control.

[0057] (2) Test time and place

[0058] The test was carried out in the company's nursery in November 2019.

[0059] (3) Test method

[0060] Treatment settings: the sunflower variety is sun spot, and the experiment set up 5 treatments, which are the strain ratio of 1:10, 1:20, 1:30, 1:50 and the control treatment without adding bacteria.

[0061] Experimental method: Accurately weigh 1g of sunflower seeds, put them into a petri dish, add 10mL of ...

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Abstract

The invention relates to bacillus firmus and an application thereof. The bacillus firmus is subjected to primary seed culture and fermentation to prepare bacillus firmus liquid inoculant, or further concentrating and sray drying are performed, so that bacillus firmus powder is prepared. The bacillus firmus inoculant has an obvious antagonistic effect on sclerotinia sclerotiorum causing sclerotiniasclerotiorum of sunflowers, can prevent or reduce sclerotinia sclerotiorum of sunflowers, promotes stable yield and income increase of the sunflowers, increases the yield of the sunflowers, and improves the quality of the sunflowers.

Description

technical field [0001] The invention belongs to the field of microbial agent products, and in particular relates to a bacillus firmus and its preparation method and application. Background technique [0002] Sunflower is one of the four major oil crops in the world. The main sunflower production areas in China are distributed in the northeast, northwest and north China, such as Inner Mongolia, Jilin, Liaoning, Heilongjiang, Shanxi and other provinces and autonomous regions. The main production area is between 35° and 55° north latitude. Sclerotinia sclerotiorum is one of the main diseases of sunflower production, which occurs to varying degrees every year, and has a great impact on the improvement of sunflower yield and quality. [0003] Sunflower sclerotinia can take place in the whole growth period of sunflower, and disease symptom common has following three types: (1) standing dry type. The disease can be infected from seedlings to the flowering stage. The symptoms are ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00A01G7/06C12R1/07
CPCC12N1/20A01N63/22A01G7/06C12R2001/07C12N1/205Y02W30/40
Inventor 肖培英顾欣燕赵晓琛刘金龙
Owner 天津开发区坤禾生物技术有限公司
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