A kind of Bacillus firmus and its preparation method and application
A technology of bacillus firmus and bacterial agent, applied in the field of microbial bacterial agent products, to achieve the effects of reducing agricultural production costs, long-lasting effect, and reducing the amount of pesticides used
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Embodiment 1
[0030] The preparation of MES80101 microbial inoculum is carried out according to the following steps:
[0031] ①First-class seed culture: The strain MES80101 activated by the slant of the test tube is cultured in an ampoule, and the ampoule medium is a nutrient agar medium. The medium formula is: peptone 8-13g, beef extract 2-5g, sodium chloride 3-5g, agar 19.5g, ultrapure water 1000ml, pH 7.0-7.5. The culture conditions were as follows: temperature 25℃-30℃, cultured for 24h.
[0032] 2. Fermentation: 20L of liquid Bacillus firmus fermentation medium was placed in a 30m3 fermenter, sterilized by moist heat at 115°C-122°C for 35-40min and cooled to 35°C, scraping the first-class seed moss in 100mL room temperature sterile water, After mixing, it was inoculated into the liquid fermentation medium of Bacillus firmus, the control tank was pressed at 0.02MPa-0.07MPa, and cultivated at 35 ° C for 26 hours; the raw materials and dosage of the medium used for fermentation were: gluc...
Embodiment 2
[0038] Bacteriostatic test of Bacillus firmus MES80101 on sunflower sclerotinia:
[0039] (1) Test time and place: In November 2019, it will be carried out in the laboratory of the company's R&D center.
[0040] (2) Test method:
[0041] (1) Source of tested strains
[0042] The pathogenic bacteria of sunflower sclerotiorum came from the sunflower farmland in Chifeng City, Inner Mongolia. After being cultivated, isolated and purified by the company's R&D personnel, it was finally identified as Sclerotinias clerotiorum (Lib.) deBary by 16S rDNA, and it was identified as strong pathogenicity by the virulence test. .
[0043] Bacillus firmus was prepared from the strain described in Example 1 through fermentation, culture and dusting.
[0044] (2) Plate antagonism experiment
[0045] Prepare a PDA solid plate, pick a sunflower sclerotinia sclerotium with a sterile inoculation needle and place it in the middle of the petri dish, burn the inoculation needle red, wait for the inoc...
Embodiment 3
[0049] Potted control effect of Bacillus firmus MES80101 on sunflower sclerotinia:
[0050] (1) Test drug
[0051] (1) Microbial agent
[0052] The MES80101 solid preparation prepared in Example 1 was diluted 10 times, 20 times, 30 times and 50 times with water respectively;
[0053] (2) Chemicals
[0054] 40% sclerotium net wettable powder 800-1200 times liquid (Shandong Keda Venture Biological Co., Ltd.).
[0055] (3) Blank control
[0056] Set clear water treatment as blank control.
[0057] (2) Test time and place
[0058] The test was conducted in the company's nursery room in November 2019.
[0059] (3) Test method
[0060] Treatment settings: The sunflower variety was sun spot, and the experiment set up 5 treatments, which were the control treatments with the bacterial species ratio of 1:10, 1:20, 1:30, 1:50 and no bacteria added.
[0061] Experimental method: Accurately weigh 1 g of sunflower seeds, put them in a petri dish, add 10 mL of pure water to immerse t...
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