Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

New application of thioether compound methyl propyl trisulfide to inhibit Phytophthora

A technology of methyl propyl trisulfide and Phytophthora capsici, applied in application, bactericide, biocide, etc., can solve problems such as environmental damage, Phytophthora drug resistance, etc. Prominent effect of suppression effect

Active Publication Date: 2021-09-24
YUNNAN AGRICULTURAL UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, chemical fungicides are often used for Phytophthora, especially Phytophthora capsici, which is easy to cause damage to the environment, and it is easy to cause Phytophthora to develop drug resistance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • New application of thioether compound methyl propyl trisulfide to inhibit Phytophthora
  • New application of thioether compound methyl propyl trisulfide to inhibit Phytophthora
  • New application of thioether compound methyl propyl trisulfide to inhibit Phytophthora

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0055] (2) Preparation of sporangia suspension: Scrape the mycelium of Phytophthora capsici from the medium cultured for 7 days with a cover glass, and rinse with sterile water into a cryopreservation tube blocked by a layer of gauze. Use a hemocytometer to adjust the concentration of the sporangia suspension to 1 x 10 4 individual / mL.

[0056] (3) Preparation of zoospore suspension: add 15ml of sterilized ddH to the culture dish for 7 days 2 O, placed in a refrigerator at 4°C for cold stimulation for 30 minutes, then allowed to stand under light at 25°C for 30 minutes, and then began to release zoospores, which were counted with a hemocytometer and diluted to make a concentration of 2×10 5 individual / mL zoospore suspension.

[0057] (4) Preparation of teleugen suspension: add 15ml of sterilized ddH to the culture dish for 7 days 2 O, placed in a refrigerator at 4°C for cold stimulation for 30 minutes, then allowed to stand under light at 25°C for 30 minutes, and then began...

Embodiment 1

[0059] Embodiment 1 The influence of methyl propyl trisulfide on the release of Phytophthora capsici zoospores

[0060] ①The preparation concentration is 1×10 4 Individual / mL Phytophthora capsici sporangia suspension;

[0061] ② Use a pipette to draw 40 μL of zoospore suspension into the groove of the concave glass slide, add 10 μL of 6 different concentrations of methyl propyl trisulfide aqueous solution, so that the final concentration of methyl propyl trisulfide is 0 μg / mL, 10μg / mL, 50μg / mL, 100μg / mL, 500μg / mL, 1000μg / mL. Place the concave glass slide on the stage of the microscope, find the zoospores with a 4x objective lens, and observe with a 10x objective lens. Count the spore release rate at 0 min, 20 min, and 40 min, and calculate the release inhibition rate. Release inhibition rate (%)=(control release rate-treatment release rate) / control release rate×100%.

[0062] The experimental results show:

[0063] When the concentration of methyl propyl trisulfide is 10...

Embodiment 2

[0064] The impact of embodiment 2 methyl propyl trisulfide on zoospore movement of Phytophthora capsici

[0065] ①The preparation concentration is 2×10 5 Individual / mL Phytophthora capsici zoospore suspension;

[0066] ② Use a pipette to draw 40 μL of zoospore suspension into the groove of the concave glass slide, and add 10 μL of 6 different concentrations of methyl propyl trisulfide aqueous solution, so that the final concentrations of methyl propyl trisulfide are respectively 0μg / mL, 10μg / mL, 50μg / mL, 100μg / mL, 500μg / mL, 1000μg / mL;

[0067] ③Under the microscope, count the cessation rate of zoospores after adding methyl propyl trisulfide for 40 minutes, and calculate the average value of the cessation rate. Stopping rate (%)=number of telospores / (number of telospores+number of zoospores)×100%. (ie motility inhibition rate).

[0068] The experimental results show:

[0069] When the concentration of methyl propyl trisulfide was 500 μg / mL, the inhibitory effect on the swi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a new application of methyl propyl trisulfide, a sulfide compound, for inhibiting Phytophthora spp., and the compound can effectively inhibit Phytophthora capsici. The compound of the present invention has remarkable effects on inhibiting the release of zoospores of Phytophthora, swimming of zoospores, germination of restospores, growth of hyphae and promoting the cracking of zoospores. The compound of the invention can be used for the prevention and control of Phytophthora in soil, preventing and inhibiting the infection of Phytophthora to plants, especially crops from the source. The compound described in the invention is a biological source compound, which can reduce the generation of Phytophthora drug resistance and reduce the environmental pollution caused by pesticides.

Description

technical field [0001] The invention relates to the field of biological control of soil-borne pathogens, in particular to a new application of methyl propyl trisulfide, a sulfide compound, for inhibiting Phytophthora capsici. Background technique [0002] Phytophthora has a wide range of hosts, is very harmful and destructive to host plants, and often leads to serious losses in crop production. Therefore, plant diseases caused by it are commonly known as "blight". For many years, the control of plant diseases has always been a difficult problem for agricultural research and production departments. Pepper blight caused by Phytophthora capsici is a worldwide disease. In addition to harming peppers, it can also infect crops such as tomatoes, eggplants, cucumbers, watermelons, and pumpkins. First discovered in the United States in 1918, the disease has spread to pepper-growing regions around the world. With the continuous expansion of the cultivation area in recent years, the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N41/12A01P3/00
CPCA01N41/12
Inventor 刘屹湘李颖朱书生韩光煜杨敏黄惠川梅馨月杜飞杨雨馨张贺吴家庆何霞红朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com