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Mesenchymal stem cell preparation as well as preparation method and application thereof

A mesenchymal stem cell and preparation technology, applied in the field of mesenchymal stem cell preparation and its preparation, can solve the problems of unstable curative effect of diabetes, hyperglycemia, and ineffective relief of diabetic complications, so as to facilitate the maintenance and preparation of cell metabolism and activity The system is easy to control and restore the effect of secretion level

Pending Publication Date: 2020-11-20
广东芙金干细胞再生医学有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, there are still some problems in the current diabetes therapy based on mesenchymal stem cells. The biggest disadvantage is that the curative effect on diabetic hyperglycemia is unstable and the relief of diabetic complications is not obvious.
A series of studies have shown that the curative effect of mesenchymal stem cells is likely to be affected by the high blood sugar and cell metabolism environment in patients. There are no relevant methods and research reports

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  • Mesenchymal stem cell preparation as well as preparation method and application thereof
  • Mesenchymal stem cell preparation as well as preparation method and application thereof
  • Mesenchymal stem cell preparation as well as preparation method and application thereof

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preparation example Construction

[0050] The mesenchymal stem cells described in the embodiments of the present invention are prepared by the following method:

[0051] (1) Weigh 1-5% human serum albumin, 0.5% low-molecular-weight heparin sodium, 1-20% compound amino acid and 0.3-0.7% vitamin C according to the mass-volume ratio, and dissolve them in the solution medium to obtain the first solution;

[0052] (2) Resuspend the mesenchymal stem cells in the first solution to form a single cell suspension, and the number of the mesenchymal stem cells is 5×10 6 -5×10 7 a / mL;

[0053] (3) Before use, the insulin is added to the single cell suspension to form a mesenchymal stem cell preparation.

[0054] Further, the present invention also provides the application of the mesenchymal stem cell preparation in the preparation of drugs for treating type 1 diabetes and type 2 diabetes and their complications.

Embodiment 1

[0057] This embodiment provides a mesenchymal stem cell preparation, the number of mesenchymal stem cells in the mesenchymal stem cell preparation is: 5×10 6 individual / mL; the mass volume ratio of human serum albumin is 1%; the mass volume ratio of low molecular weight heparin sodium is 0.5%; the mass volume ratio of compound amino acid is 1%; the mass volume ratio of vitamin C is 0.5%; the dosage of insulin is : 0.5U / kg, the balance is compound electrolyte solution.

[0058] The embodiment of the present invention further provides a preparation method for the above-mentioned mesenchymal stem cell preparation, including the following steps: preparing 100ml of mesenchymal stem cell preparation, which is composed of 5ml of human serum albumin stock solution (final concentration is 1%), low molecular weight heparin sodium 0.5ml (final concentration 0.5%), compound amino acid 1ml (final concentration 1%), vitamin C 0.5g (final concentration 0.5%), compound electrolyte solution 93...

Embodiment 2

[0060] This embodiment provides a mesenchymal stem cell preparation, the number of mesenchymal stem cells in the mesenchymal stem cell preparation is: 1×10 7 individual / mL; the mass volume ratio of human serum albumin is 5%; the mass volume ratio of low molecular weight heparin sodium is 0.5%; the mass volume ratio of compound amino acid is 20%; the mass volume ratio of vitamin C is 0.5%; the dosage of insulin is : 0.5U / kg, the balance is 5% glucose injection.

[0061] The embodiment of the present invention further provides a preparation method for the above-mentioned mesenchymal stem cell preparation, including the following steps: preparing 100ml of mesenchymal stem cell preparation, which is composed of 25ml of human serum albumin stock solution (final concentration is 5%), low molecular weight heparin sodium 0.5ml (final concentration 0.5%), compound amino acid 20ml (final concentration 20%), vitamin C 0.5g (final concentration 0.5%), 5% glucose injection 54ml and mesench...

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Abstract

The invention provides a mesenchymal stem cell preparation as well as a preparation method and application thereof. Mesenchymal stem cells are derived from human umbilical cords, the mesenchymal stemcell preparation is composed of human mesenchymal stem cells, human blood albumin, low-molecular heparin sodium, compound amino acid, vitamin C, insulin and a solution medium, and the solution mediumcan be a compound electrolyte solution or a glucose solution. According to the invention, the human mesenchymal stem cell preparation is used for repairing damaged pancreatic beta cells, and the secretion of endogenous insulin is used for reducing blood sugar, so that the purpose of relieving hyperglycemia is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a mesenchymal stem cell preparation capable of alleviating hyperglycemia, a preparation method and application thereof. Background technique [0002] Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia caused by defective insulin secretion or its biological action, or both. According to the "Global Diabetes Report" released by the World Health Organization in 2016, the number of people with diabetes is increasing worldwide. In my country, there are currently 129 million diabetic patients, with a prevalence rate of 9.4%, which is higher than the global average prevalence rate of 8.5%, including 10.5% for men and 8.3% for women. [0003] At present, the treatment of diabetes mainly relies on diet control, exercise therapy, drugs and insulin comprehensive treatment. The comprehensive application of these therapies can control blood sug...

Claims

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Application Information

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IPC IPC(8): A61K38/28A61K47/36A61K47/42A61P3/10A61P9/10A61P13/12A61P19/10A61P25/02A61P31/00A61K31/375A61K31/727A61K35/28
CPCA61K35/28A61K38/28A61K31/727A61K31/375A61K47/42A61K47/36A61P3/10A61P19/10A61P13/12A61P9/10A61P25/02A61P31/00A61K2300/00
Inventor 高迎凤胡成虎安欢笑
Owner 广东芙金干细胞再生医学有限公司
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