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Application of a Fluorescent Conjugated Polymer Nanoprobe in Peripheral Nerve Imaging

A conjugated polymer and nanoprobe technology, which is applied to preparations and pharmaceutical formulations for in vivo experiments, to achieve the effects of simple cleaning method, high biological safety, and strong clinical application value.

Active Publication Date: 2021-07-30
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides an application method of a fluorescent conjugated polymer nanoprobe in peripheral nerve imaging, designs and prepares a fluorescent conjugated polymer nanoprobe, and adopts a simple and convenient method to realize efficient fluorescence imaging of mammalian peripheral nerves , to overcome the problem that the existing neurofluorescence imaging contrast agent has considerable uptake in nerves and surrounding tissues, and can realize stable navigation of peripheral nerves during surgery with high safety

Method used

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  • Application of a Fluorescent Conjugated Polymer Nanoprobe in Peripheral Nerve Imaging
  • Application of a Fluorescent Conjugated Polymer Nanoprobe in Peripheral Nerve Imaging
  • Application of a Fluorescent Conjugated Polymer Nanoprobe in Peripheral Nerve Imaging

Examples

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Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Preparation and performance characterization of fluorescent conjugated polymer nanoprobes

[0062] Take 0.25 mL of poly[(9,9-dioctylfluorenyl-2,7diyl)-(1,4-benzo-{2,1',3}-thiadiazole) at a concentration of 1 mg / mL ] 10% benzothiadiazole (y) (PFBT) solution and 0.25 mL of amino-terminated polymethyl methacrylate (MMA-NH 2 ) solution was added to 1.5mL tetrahydrofuran (THF), in which PFBT stock solution and MMA-NH 2 The storage solution was prepared with THF as solvent. The mixture was sonicated in a water bath for 5 minutes. Under the conditions of probe sonication, the above mixture was quickly transferred to 10 mL of deionized water, and the probe was sonicated for 1 minute. Under the condition of 45℃, N 2 Remove all THF in the solution in 40 minutes to prepare 0.025mg / mL PFBT-NH 2 Fluorescent Conjugated Polymer Nanoprobes.

[0063] Take 0.25 mL of poly[(9,9-dioctylfluorenyl-2,7diyl)-(1,4-benzo-{2,1',3}-thiadiazole) at a concentration of 1 mg / mL ] 10%...

Embodiment 2

[0066] Example 2 Fluorescent Conjugated Polymer Nanoprobes Imaging Mammalian Peripheral Nerves

[0067] The imaging methods of fluorescent conjugated polymer nanoprobes for mammalian peripheral nerves include direct exposure imaging and intramuscular injection imaging, respectively as follows:

[0068] 1. Direct Exposure Imaging

[0069] The mouse sciatic nerve is surgically exposed by removing the cutaneous muscle and fatty tissue overlying the sciatic nerve. 50 μL aqueous solution of fluorescent conjugated polymer nanoprobe was dropped on the exposed left sciatic nerve and right sciatic nerve, covering all tissues (nerve, muscle, fat and fascia) in the surgically exposed area, and the left sciatic nerve was dripped with PFBT -COOH fluorescent conjugated polymer nanoprobe aqueous solution, the right sciatic nerve was dripped with PFBT-NH 2 Fluorescent conjugated polymer nanoprobe aqueous solution, after incubation for a certain period of time, remove each probe aqueous solu...

Embodiment 3

[0075] Example 3 Determination of the binding position of the conjugated polymer nanoprobe with the mouse sciatic nerve

[0076] After the in vivo fluorescence imaging was completed, the mouse sciatic nerve was taken out for the in vitro slice experiment. After embedding in OCT (a water-soluble mixture of polyethylene glycol and polyvinyl alcohol), place it in a -80°C refrigerator for more than 4 hours, cut the tissue into 10 μm thick sections, then perform HE staining, and perform fluorescence on the tissue after sealing. imaging.

[0077] Figure 8 , Figure 9 , Figure 10 Diagram of isolated tissue slices of sciatic nerve in the experimental group, Figure 8 It is the fluorescence imaging and HE staining image of mouse sciatic nerve cross section, Figure 9 It is the fluorescence imaging and HE staining imaging of the longitudinal section of the mouse sciatic nerve. It can be seen from the results of the picture that the probe mainly gathers in the outer nerve model 3 ...

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Abstract

The invention discloses the application of a fluorescent conjugated polymer nanoprobe in peripheral nerve imaging, and relates to the field of fluorescent conjugated polymer nanotechnology. The application method includes: preparing a fluorescent conjugated polymer nanoprobe, the probe Including general fluorescent conjugated polymer nanoprobes and near-infrared fluorescent conjugated polymer nanoprobes; fluorescent conjugated polymer nanoprobes are formulated into fluorescent conjugated polymer nanoprobe colloidal aqueous solutions; applied to mammals Neuroimaging, including direct exposure imaging and intramuscular injection imaging. The preparation process of the conjugated polymer nanoprobe adopted in the present invention is simple, and it is applied to peripheral nerve imaging. The conjugated polymer nanoprobe concentrates on the epineurium and perineurium to mark nerves, does not damage nerve cells, and has high biological safety; It can achieve high-efficiency visualization of mammalian peripheral nerves, without non-specific uptake of fat tissue and skin tissue around the nerves, and has strong clinical application value.

Description

technical field [0001] The invention relates to the field of fluorescent conjugated polymer nanotechnology, in particular to the application of a fluorescent conjugated polymer nanoprobe in peripheral nerve imaging. Background technique [0002] Injury to the peripheral nervous system is a major problem in surgery and is one of the most feared surgical complications. Especially in malignant tumor resection, the physiological distortion around the malignant lesion increases the chance of peripheral nerve damage. There are 600,000 neurological injuries caused by surgical procedures each year in the United States alone. Injuries to the peripheral nervous system greatly affect the quality of life of patients, and have a huge impact on patients' physiology and psychology. During thyroidectomy, for example, the laryngeal nerve is often injured, which can lead to a change or loss of the patient's voice or respiratory problems. In head and neck cancer, peripheral nervous system d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
CPCA61K49/0054
Inventor 张菊香唐诗逸熊丽琴
Owner SHANGHAI JIAOTONG UNIV
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