Osteoinductive active extract and preparation method thereof

An extract and osteoinductive technology, which is applied in the fields of pharmaceutical formulation, drug delivery, and medical science, can solve the problems of complexity, increased production costs, and many types of ingredients, and achieve low production costs, short production cycles, and improved osteoinductive production. The effect of bone activity

Pending Publication Date: 2020-12-18
TIANJIN ZHONGJIN BIOLOGICAL DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disclosed composition also includes biologically active agents, and the ingredients are many and complex, and a large amount of dilute acid solution and protease are used in the preparation process, which increases the production cost

Method used

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  • Osteoinductive active extract and preparation method thereof
  • Osteoinductive active extract and preparation method thereof
  • Osteoinductive active extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] A preparation method of an osteoinductive active extract, comprising the steps of:

[0072] (1) Pulverization: The cortical bone block is pulverized at low temperature to form cortical bone particles, and the pulverization step uses liquid nitrogen to keep the temperature at -20°C;

[0073] (2) Degreasing: Add cortical bone particles to the reactor, then add methanol equivalent to 0.3 times the weight of cortical bone particles, and 0.7 times chloroform, react at room temperature for 5 hours, replace the degreasing solution, degrease again, and filter to obtain the degreased cortical bone particles;

[0074] (3) Decalcification: soak the degreased cortical bone particles obtained in step (2) completely in a decalcification solution (0.4mol / L hydrochloric acid), put them in an ultrasonic reactor at room temperature for 2 hours, and replace the decalcification solution again Decalcified twice, filtered to obtain cortical bone particles after decalcification;

[0075] (4...

Embodiment 2

[0086] A preparation method of an osteoinductive active extract is basically the same as in Example 1, the difference being:

[0087] (1) Pulverization: The cortical bone blocks are pulverized at low temperature to form cortical bone particles, and the pulverization step uses liquid nitrogen to keep the temperature at -22°C;

[0088] (5) Extraction: the cortical bone particles obtained in step (4) after decontamination are added to the extract, and the extract is obtained by ultrasonic reaction at room temperature, the extract is concentrated, and the concentrated extract is extracted at room temperature under dialysis;

[0089] The preparation method of the extract is as follows: add urea 0.6g, calcium chloride 150g, alpha-lipoic acid 15g, guanidine hydrochloride 60g into water and stir until dissolved, dilute to 1L with water, then add N-ethylmaleimide 1.5 g of amine to obtain the extract.

[0090] The osteoinductive activity extract obtained in Implementation 2 was analyz...

Embodiment 3

[0092] A preparation method of an osteoinductive active extract is basically the same as in Example 1, the difference being:

[0093] (1) Pulverization: The cortical bone blocks are pulverized at low temperature to form cortical bone particles, and the pulverization step uses liquid nitrogen to maintain the temperature at -21°C;

[0094] (2) Degreasing: Add cortical bone grains to the reactor, then add methanol equivalent to 0.3 times the weight of cortical bone grains, and chloroform 0.7 times the weight, react at room temperature for 6 hours, replace the degreasing solution, degrease again, and filter to obtain the degreased cortical bone particles;

[0095] (5) Extraction: the cortical bone particles obtained in step (4) after decontamination are added to the extract, and the extract is obtained by ultrasonic reaction at room temperature, the extract is concentrated, and the concentrated extract is extracted at room temperature under dialysis;

[0096] The preparation met...

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Abstract

The invention provides a preparation method of an osteoinductive active extract and the prepared osteoinductive active extract. The osteoinductive active extract is prepared from the following components in percentage by mass: 0.91-4.3% of an accelerant; 2.56-23.85% of an inducer; 11.22-28.85% of an activator ; 16.4-56.4% of a regulator and 0.55-3.8% of a bacteriostatic agent; the invention further provides a preparation method of the osteoinductive active extract. The osteoinductive active extract and the preparation method thereof are short in production cycle, low in production cost and suitable for industrial large-scale production, can be applied to bone repair materials, can better improve the osteoinductive osteogenic activity of the bone repair materials, and have remarkable effects when being applied to clinical treatment of bone nonunion, delayed bone healing and bone nonunion.

Description

technical field [0001] The invention belongs to the field of preparation of bone repair materials, and in particular relates to an osteoinductive active extract and a preparation method thereof. Background technique [0002] Clinically, the repair treatment of bone defect, nonunion and nonunion caused by bone trauma, bone tumor or other surgery requires the use of bone repair materials. The clinically desired bone repair material is capable of supporting and filling, and can be degraded at an appropriate speed. Under a suitable three-dimensional network structure and certain inducing factors, blood vessels can grow in, autogenous bone can form, and thus crawl. Alternative bone repair materials. During the whole process, it is not desirable to have reactions such as foreign body occupation and rejection. [0003] Autologous bone grafts have good biocompatibility and excellent biological activity, but the limited materials can also cause new injuries, defects and pain. The ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/22A61L27/20A61L27/24A61L27/50A61L27/54
CPCA61L27/365A61L27/3608A61L27/3687A61L27/3691A61L27/227A61L27/20A61L27/24A61L27/50A61L27/54A61L2300/412A61L2430/02A61L2300/414A61L2300/252A61L2300/254A61L2300/232A61L2300/406A61L2400/06
Inventor 李湘杰任芳丁力朱晓丽刘红云聂卫祝君梅盖增王长江
Owner TIANJIN ZHONGJIN BIOLOGICAL DEV CO LTD
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