Method for extracting lentinan by two-step enzyme, lentinan extract and application of lentinan extract

A technology of lentinan and extract, which is applied in the field of two-step enzymatic extraction of lentinan and lentinan extract, to achieve the effects of increasing polysaccharide yield, promoting separation, and increasing permeability

Pending Publication Date: 2020-12-18
周银根
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the prevention and treatment of Parkinson's disease by lentinan

Method used

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  • Method for extracting lentinan by two-step enzyme, lentinan extract and application of lentinan extract
  • Method for extracting lentinan by two-step enzyme, lentinan extract and application of lentinan extract
  • Method for extracting lentinan by two-step enzyme, lentinan extract and application of lentinan extract

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Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1 Two-step enzyme extraction method of Lentinan of the present invention (1)

[0047] 1. Extraction of Lentinan

[0048] Follow the steps below to extract lentinan:

[0049] 1) Select clean shiitake mushrooms, carefully remove debris and dry in the sun, totaling 100kg, crushed to 30 mesh;

[0050] 2) Soaking: Process the drinking water of urban residents into pure water, and soak at room temperature for 3 hours with a material-to-liquid ratio of 1:10;

[0051] 3) Enzyme extraction: add cellulase and food-grade ethanol, the amount of cellulase added is 800U / g, and the final volume concentration of food-grade ethanol is set to 0, 2%, 4%, 5%, 6%, 8% %, 10%, 12% eight gradients, temperature 40°C, react in an enzyme reactor for 10 hours, the reaction process requires intermittent stirring, 100 revolutions per minute, and the stop ratio is 1:1;

[0052] 4) Slag-liquid separation: the extract in the enzyme reactor enters the horizontal unloading centrifuge, and t...

Embodiment 2

[0072] Embodiment 2 The method (two) of two-step enzyme extracting lentinan of the present invention

[0073] 1. Extraction of Lentinan

[0074] Follow the steps below to extract lentinan:

[0075] 1) Choose clean shiitake mushrooms, carefully remove sundries, dry in the sun, total 100kg, crush to 40 mesh;

[0076] 2) Soaking: Process the drinking water of urban residents into pure water, and soak at room temperature for 2 hours with a material-to-liquid ratio of 1:10;

[0077] 3) Enzyme extraction: add cellulase and food-grade ethanol, the amount of cellulase added is 900U / g, the final volume concentration of food-grade ethanol is 5%, the temperature is 45 ° C, react in an enzyme reactor for 8 hours, and react The process requires intermittent stirring, 100 revolutions per minute, and the stop ratio is 1:1;

[0078] 4) Slag-liquid separation: the extract in the enzyme reactor enters the horizontal unloading centrifuge, and the separated extract enters the conical storage t...

Embodiment 3

[0089] Embodiment 3 The method (three) of two-step enzyme extracting lentinan of the present invention

[0090] 1. Extraction of Lentinan

[0091] Follow the steps below to extract lentinan:

[0092] 1) Select clean shiitake mushrooms, carefully remove debris and dry in the sun, a total of 100kg, crushed to 20 mesh;

[0093] 2) Soaking: Process the drinking water of urban residents into pure water, and soak at room temperature for 4 hours with a material-to-liquid ratio of 1:10;

[0094] 3) Enzyme extraction: add cellulase and food-grade ethanol, the addition of cellulase is 600U / g, the final volume concentration of food-grade ethanol is 10%, the temperature is 35 ° C, react in an enzyme reactor for 12 hours, and the reaction The process requires intermittent stirring, 100 revolutions per minute, and the stop ratio is 1:1;

[0095] 4) Slag-liquid separation: the extract in the enzyme reactor enters the horizontal unloading centrifuge, and the separated extract enters the co...

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Abstract

The invention relates to a method for extracting lentinan by a two-step enzyme, a lentinan extract and application of the lentinan extract. The method comprises the following steps: first conducting extraction by using cellulase and an ethanol activator at 35-45 DEG C, carrying out secondary extraction on the slag by using purified water, merging the extracting solutions, regulating the pH value to about 6-6.8, standing at 40-50 DEG C, carrying out polymerization reaction for more than 12 hours, removing the precipitate, carrying out vacuum concentration on the supernatant to obtain an extractwith the specific gravity of 1.2-1.25, and finally carrying out drying under the condition of the upper temperature limit <= 60 DEG C, and the lower temperature limit >= 50 DEG C. The method is convenient for industrial implementation, the polysaccharide yield and the unit content are remarkably improved, the extract is a water full-soluble substance, and effective components in the raw materialsare reserved to the maximum extent. Cell and animal tests prove that the lentinan extract has a good prevention and treatment effect on Parkinson's disease.

Description

technical field [0001] The invention relates to the field of extraction of plant active components, in particular to a two-step enzyme extraction method of lentinan, lentinan extract and application thereof. Background technique [0002] Shiitake mushrooms are natural plants that can be used for both medicine and food. From wild picking to artificial planting, they have left a very good reputation for many years. Based on the results of modern technological research, it has been basically analyzed that mushrooms are rich in amino acids, not only in a complete range but also in more than 12% of the amino acids. In addition to nutrition and nourishment, shiitake mushrooms also have medicinal functions, and they contain high-molecular-weight polysaccharides. Lentinan is a polysaccharide extracted from the fruiting body of Lentinus edodes. It is a polymer of more than ten kinds of sugar functional groups such as galactose, mannose, rhamnose, xylose, arabinose, and β-pyranose. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04C08B37/02A61K31/715A61P25/16
CPCA61K31/715A61P25/16C08B37/0003C08B37/0024C12P19/04C12P19/14
Inventor 周银根丁汉升
Owner 周银根
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