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3D-printable epsilon-polylysine antibacterial hydrogel as well as preparation method and application of 3D-printable epsilon-polylysine antibacterial hydrogel

A polylysine, 3D printing technology, used in medical science, prosthesis, additive processing, etc., can solve the problems of hydrogel cytotoxicity, insufficient mechanical strength, poor biocompatibility, etc. The effect of fast glue speed and mild operating conditions

Pending Publication Date: 2020-12-22
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molding methods of the prior art are often not gentle enough, or the hydrogel after cross-linking molding will have a certain degree of cytotoxicity, and there are problems such as poor biocompatibility or insufficient mechanical strength.

Method used

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  • 3D-printable epsilon-polylysine antibacterial hydrogel as well as preparation method and application of 3D-printable epsilon-polylysine antibacterial hydrogel
  • 3D-printable epsilon-polylysine antibacterial hydrogel as well as preparation method and application of 3D-printable epsilon-polylysine antibacterial hydrogel
  • 3D-printable epsilon-polylysine antibacterial hydrogel as well as preparation method and application of 3D-printable epsilon-polylysine antibacterial hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Add methacrylic acid in deionized water at 65°C. After it is completely dissolved, add the molar ratio of EDC, NHS and MA to the solution at 25°C to activate the carboxyl group on the methacrylic acid. For 30 minutes, add ε-polylysine (ε-PL, molecular weight 3700 Daltons) into the mixture at 40°C, the mass concentration of ε-PL is 10g / L, stir to dissolve, ε-PL and MA The molar ratio was 1:1, the pH value of the reaction system was adjusted to 4.5, and the reaction was carried out at 40°C for 15h. The obtained polymer was transferred to a dialysis bag, placed in deionized water and dialyzed for 3 days, and the purified solution obtained after dialysis was freeze-dried to obtain a methacrylic acid-modified ε-polylysine polymer (ε-PL-MA) , the grafting rate was 13.4%.

[0047] (2) carboxymethyl cellulose is dissolved in deionized water, the mass concentration of CMC is 10g / L, stirring and dissolving; Then add glycidyl methacrylate (GMA), the mol ratio of CMC and GMA i...

Embodiment 2

[0050] (1) Add methacrylic acid in deionized water at 65°C. After it is completely dissolved, add the molar ratio of EDC and NHS to MA into the solution at 25°C to activate the activation of methacrylic acid. Carboxyl for 30 minutes, add ε-polylysine (ε-PL, molecular weight 3600 Daltons) into the mixture at 45°C, the mass concentration of ε-PL is 10g / L, stir to dissolve, ε-PL and The molar ratio of MA was 1:2, the pH value of the reaction system was adjusted to 5, and the reaction was carried out at 45°C for 20h. The obtained polymer was transferred to a dialysis bag, placed in deionized water and dialyzed for 3 days, and the purified solution obtained after dialysis was freeze-dried to obtain a methacrylic acid-modified ε-polylysine polymer (ε-PL-MA) , the grafting rate was 16.3%.

[0051] (2) carboxymethyl cellulose is dissolved in deionized water, the mass concentration of CMC is 20g / L, stirring and dissolving; Then add glycidyl methacrylate (GMA), the mol ratio of CMC and...

Embodiment 3

[0054] (1) Add methacrylic acid in deionized water at 65°C. After it is completely dissolved, add it into the solution at 25°C according to the molar ratio of EDC / NHS and MA at 1:1.5 to activate the activation of methacrylic acid. Carboxyl group for 30 minutes, add ε-polylysine (ε-PL, molecular weight 4000 Daltons) into the mixture at 50°C, the mass concentration of ε-PL is 20g / L, stir to dissolve, ε-PL and The molar ratio of MA was 1:2, the pH value of the reaction system was adjusted to 4, and the reaction was carried out at 50°C for 24h. The obtained polymer was transferred to a dialysis bag, placed in deionized water and dialyzed for 5 days, and the obtained purified solution after dialysis was freeze-dried to obtain a methacrylic acid-modified ε-polylysine polymer (ε-PL-MA) , the grafting rate was 18.7%.

[0055] (2) carboxymethyl cellulose is dissolved in deionized water, the mass concentration of CMC is 10g / L, stirring and dissolving; Then add glycidyl methacrylate (GM...

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Abstract

The invention discloses 3D-printable epsilon polylysine antibacterial hydrogel as well as a preparation method and application of the 3D-printable epsilon polylysine antibacterial hydrogel. The preparation method comprises the following steps: mixing a methacrylic acid-modified epsilon polylysine polymer, a glycidyl methacrylate-modified carboxymethyl cellulose polymer and an aqueous solution containing a photoinitiator to obtain a mixed solution; and then injecting the mixed solution into a 3D printer for 3D printing, and conducting irradiating with ultraviolet light while printing. Accordingto the preparation method, carbon-carbon double bonds on methacrylic acid and glycidyl methacrylate are used for inducing free radical polymerization through ultraviolet light in the presence of thephotoinitiator, so epsilon polylysine and the carboxymethyl cellulose macromolecular polymer are crosslinked together to form the hydrogel with a three-dimensional network structure. The hydrogel hasgood mechanical properties, good biocompatibility and biodegradability, and implementation conditions are mild and controllable. The specific antibacterial activity of epsilon polylysine has an inhibition effect on Gram-negative bacteria and Gram-positive bacteria, so the hydrogel can effectively prevent wound infection and can be applied to the fields of medical dressings, tissue engineering materials and the like.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a 3D-printable photocrosslinked ε-polylysine antibacterial hydrogel, a preparation method thereof, and an application thereof. Specifically, the hydrogel porous scaffold material is obtained by free radical polymerization and crosslinking under the condition of ultraviolet light through double bond free radical polymerization. Background technique [0002] The emergence of skin tissue engineering has opened up a new way for the repair and reconstruction of large area skin damage. Hydrogel has become a research hotspot because of its porous structure and wet and soft properties. Hydrogel can simulate cell matrix, has similar viscoelasticity and mechanical strength to skin, and because of its good biocompatibility, it can promote cell growth and reproduction, and effectively promote skin wound healing. It is regarded as an ideal skin tissue replacement material and...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08J3/24C08J3/28C08L77/04C08L1/28A61L26/00A61L27/26A61L27/50A61L27/52A61L27/54B33Y70/00
CPCC08J3/075C08J3/246C08J3/28A61L27/52A61L27/54A61L27/26A61L27/50A61L26/0052A61L26/0066A61L26/008A61L26/0085A61L26/0061B33Y70/00C08J2377/04C08J2301/28C08J2477/04A61L2300/404C08L77/04C08L1/28C08J2377/00
Inventor 迟波王晓雪
Owner NANJING UNIV OF TECH
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