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Application of salidroside in preparation of medicine for preventing and treating cardiovascular diseases caused by coronary artery endothelial cell injury

A technology for the prevention and treatment of cardiovascular and salidroside, applied in the field of biomedicine, can solve the problems that have not yet been discovered, and achieve the effects of up-regulating activity, scavenging oxygen free radicals, and inhibiting cell apoptosis

Inactive Publication Date: 2021-01-01
AFFILIATED HOSPITAL OF GUANGDONG MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is mentioned in the prior art that salidroside has antihypertensive effect in vivo (CN103463115A), can prepare medicine for treating diabetic foot (CN105535001A) and estrogen receptor inhibitor (CN106581018A), and has the function of preventing and / or treating coronary artery Efficacy for microangiopathies (CN110613722A), but so far no report of Rhodiola rosea and its active ingredients being used to prepare drugs for preventing and treating cardiovascular diseases caused by coronary artery endothelial cell damage

Method used

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  • Application of salidroside in preparation of medicine for preventing and treating cardiovascular diseases caused by coronary artery endothelial cell injury
  • Application of salidroside in preparation of medicine for preventing and treating cardiovascular diseases caused by coronary artery endothelial cell injury
  • Application of salidroside in preparation of medicine for preventing and treating cardiovascular diseases caused by coronary artery endothelial cell injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Intervention effect of salidroside on hypoxia-reoxygenation injury of coronary artery endothelial cells

[0029] 1. Experimental materials

[0030] 1. Experimental animals: Human coronary artery endothelial cells (HCAECs) were purchased from Sciencell, USA.

[0031] 2. Main reagent: salidroside (SAL, sigma company, USA).

[0032] 3. Main experimental instruments: biological safety cabinet (Thermo Fisher Scientific Co., Ltd. of the United States), Sanqi cell incubator (Thermo Fisher Scientific Co., Ltd. of the United States), OLYMPUS laser scanning confocal microscope (Olympus Company of Japan), Epoch microplate reader (German Gene Co., Ltd.), high-speed low-temperature desktop centrifuge (Thermo Fisher Scientific Co., Ltd., USA), and electronic balance (China Sartorius Scientific Instrument Co., Ltd.).

[0033] 2. Experimental method

[0034] 1. Culture of HCAECs cells

[0035] (1) Cultivate with ECM medium, wait for the cells to grow to a confluence of 80%-90%, and...

Embodiment 2

[0055] Salidroside reduces coronary artery endothelial cell death

[0056] 1. Experimental materials

[0057] 1. Experimental animals: Human coronary artery endothelial cells (HCAECs) were purchased from Sciencell, USA.

[0058] 2. Main reagent: salidroside (SAL, sigma company, USA).

[0059] 3. Main experimental instruments: biological safety cabinet (Thermo Fisher Scientific Co., Ltd. of the United States), Sanqi cell incubator (Thermo Fisher Scientific Co., Ltd. of the United States), OLYMPUS laser scanning confocal microscope (Olympus Company of Japan), Epoch microplate reader (German Gene Co., Ltd.), high-speed low-temperature desktop centrifuge (Thermo Fisher Scientific Co., Ltd., USA), and electronic balance (China Sartorius Scientific Instrument Co., Ltd.).

[0060] 2. Experimental method

[0061] 1. Cytotoxicity detection of lactate dehydrogenase (LDH)

[0062] (1) Prepare LDH detection working solution and 1×LDH release reagent in advance as required;

[0063] (...

Embodiment 3

[0072] Salidroside up-regulates the activity of antioxidant system in coronary artery endothelial cells after hypoxia-reoxygenation injury

[0073] 1. Experimental materials

[0074] 1. Experimental animals: Human coronary artery endothelial cells (HCAECs) were purchased from Sciencell, USA.

[0075] 2. Main reagent: salidroside (SAL, sigma company, USA).

[0076] 3. Main experimental instruments: biological safety cabinet (Thermo Fisher Scientific Co., Ltd. of the United States), Sanqi cell incubator (Thermo Fisher Scientific Co., Ltd. of the United States), OLYMPUS laser scanning confocal microscope (Olympus Company of Japan), Epoch microplate reader (German Gene Co., Ltd.), high-speed low-temperature desktop centrifuge (Thermo Fisher Scientific Co., Ltd., USA), and electronic balance (China Sartorius Scientific Instrument Co., Ltd.).

[0077] 2. Experimental method

[0078] 1. ROS and O 2 · - level detection

[0079] (1) Select the optimal concentration of SAL as 60 μ...

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Abstract

The invention relates to application of salidroside in preparation of a medicine for preventing and treating cardiovascular diseases caused by coronary artery endothelial cell injury, and belongs to the field of biological medicines. The invention provides the application of the salidroside in preparation of a medical product for preventing and treating cardiovascular diseases caused by coronary artery endothelial cell injury. The salidroside can significantly remove oxygen free radicals of coronary endothelial cells, up-regulate the activity of an antioxidant system, promote autophagy and inhibit cell apoptosis to improve coronary endothelial dysfunction caused by anoxia / reoxygenation, which indicates that the salidroside can protect coronary artery endothelial cells and relieve cardiovascular disease symptoms related to coronary artery endothelial injury.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application of salidroside in the preparation of medicines for preventing and treating cardiovascular diseases caused by coronary artery endothelial cell damage. Background technique [0002] Coronary arteriosclerotic heart disease occurs more frequently in adults over the age of 40, and the onset is earlier in men than in women, and the incidence is higher in economically developed countries. Sustained and severe ischemia and hypoxia can cause cardiac necrosis, which is myocardial infarction (MI). Due to the high cost of treatment, it brings a heavy economic burden to patients and families, and hinders the progress and development of society. Although the popularity of interventional therapy and coronary artery bypass grafting can alleviate the initial myocardial damage in the acute stage, the sudden opening of the coronary artery and the restoration of blood flow, as ...

Claims

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Application Information

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IPC IPC(8): A61K31/7032A61P9/10A61P9/04A61P9/06A23L33/105
CPCA23V2002/00A61K31/7032A23L33/105A61P9/04A61P9/06A61P9/10A23V2200/326A23V2250/21
Inventor 雷桅何原税晓容兰小中
Owner AFFILIATED HOSPITAL OF GUANGDONG MEDICAL UNIV
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