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Preparation method and application of animal marrow effective part

A technology of effective parts and animal bones, applied to the preparation of effective parts of animal bone marrow and its application field

Pending Publication Date: 2021-01-01
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction, separation and structural identification of the chemical components of bovine bone marrow and horse bone marrow and their related pharmacological research are still in the initial stage, especially macromolecular compounds such as polysaccharides and proteins

Method used

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  • Preparation method and application of animal marrow effective part
  • Preparation method and application of animal marrow effective part
  • Preparation method and application of animal marrow effective part

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] a. Take the horse leg bone sample and store it in the refrigerator at -4°C to harden the horse bone marrow into a block, which is convenient for enrichment. Separate the horse bone and bone marrow with a hand-held cutter and knife, wash 3 times, and remove blood stasis and impurities. Horse bone marrow was collected after the animal;

[0030] b. Take the horse bone marrow obtained in step a, cut it into small pieces with a knife, put the small pieces of horse bone marrow in a pounding pot at a ratio of 1:6g / mL, add liquid nitrogen to freeze, grind and pulverize into fine powder, and put it in the refrigerator at -4°C Preserve, obtain horse bone marrow powder;

[0031] c. Extract the horse bone marrow powder obtained in step b with hot water reflux, the ratio of solid to liquid is 1:5, the extraction temperature is 90°C, extract twice, combine the extracts, centrifuge at 7000rpm for 10min, take the supernatant, concentrate, Precipitate with ethanol at a volume ratio of ...

Embodiment 2

[0036] a. Take bovine bone samples and store them in a refrigerator at -4°C to harden the bovine bone marrow into blocks for easy enrichment. Separate the bovine bone and bovine bone marrow with a hand-held cutter and knife, wash 5 times, and remove blood stasis and sundries Bovine bone marrow was collected afterwards;

[0037] b. Take the bovine bone marrow obtained in step a, cut it into small pieces with a knife, put the small piece of bovine bone marrow in a pounding pot at a ratio of 1:6g / mL, add liquid nitrogen to freeze, grind and pulverize into fine powder, and put it in the refrigerator at -4°C Preserve to obtain bovine bone marrow powder;

[0038] c. Extract the bovine bone marrow powder obtained in step b with hot water reflux, the ratio of solid to liquid is 1:10, the extraction temperature is 90°C, extract twice, combine the extracts, centrifuge at 7000rpm for 10min, take the supernatant, concentrate, Precipitate with ethanol at a volume ratio of 1:4, place at 4°...

Embodiment 3

[0043] a. Take horse bone samples and store them in a refrigerator at -4°C to harden the horse bone marrow into blocks for easy enrichment. Separate the horse bone and horse bone marrow with a hand-held cutter and knife, wash 4 times, and remove blood stasis and impurities. Horse bone marrow was collected after the animal;

[0044] b. Take the horse bone marrow obtained in step a, cut it into small pieces with a knife, put the small pieces of horse bone marrow in a pounding pot at a ratio of 1:6g / mL, add liquid nitrogen to freeze, grind and pulverize into fine powder, and put it in the refrigerator at -4°C Save to get horse bone marrow powder;

[0045] c. Extract the horse bone marrow powder obtained in step b with hot water reflux, the ratio of solid to liquid is 1:10, the extraction temperature is 90°C, extract twice, combine the extracts, centrifuge at 7000rpm for 10min, take the supernatant, concentrate, Precipitate with ethanol at a volume ratio of 1:4, place at 4°C over...

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Abstract

The invention relates to a preparation method and application of an animal marrow effective part. Raw materials in the method are fresh marrows contained in animal bones in slaughter houses of cattle,sheep, horses, camels or other animals, wherein a preparation method of a horse marrow effective component and application of the horse marrow effective component to osteoporosis are mainly used. According to the method, horse marrows are subjected to liquid nitrogen freezing, grinding, crushing, sieving, separation and petroleum ether degreasing treatment, water is used as a solvent, effective components in the horse marrows are extracted, and extracting solution is subjected to centrifugation, concentration, absolute ethyl alcohol precipitation, centrifugation and vacuum freezing drying toprepare crude extract; and the crude extract is respectively subjected to dialysis as well as separation and purification of ion exchange resin and the like to prepare the horse marrow effective component. The effective part is verified to have activities of changing hemorheology, improving microcirculation, reducing pain, having free radical scavenging capacity and the like. The research can provide reference for development of the animal marrow effective components as osteoporosis health-care products for treating or preventing various joint stiffness diseases and application of the animal marrow effective components to a medicine for treating oxidative stress mediated postmenopausal osteoporosis.

Description

technical field [0001] The invention relates to a preparation method and application of an effective fraction of animal bone marrow. Background technique [0002] Osteoporosis is a systemic metabolic chronic skeletal disease characterized by decreased bone mass and bone microstructural degeneration and decreased bone density. When the bone becomes more porous and fragile, the risk of fracture increases greatly. When cells are disturbed and their oxidation-reduction reaction is unbalanced, a large number of free radicals and peroxides will be produced, and the accumulated excessive free radicals will undergo oxidation-reduction reactions with macromolecules in the body, resulting in damage to cell structure and function. Changes can lead to the emergence of various diseases. Studies have confirmed that there is a certain relationship between oxidative stress and the occurrence of osteoporosis, and substances with antioxidant effects can prevent and treat the occurrence of os...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P19/02A61P19/10A61P39/06A23L33/00
CPCA61K35/28A61P19/02A61P19/10A61P39/06A23L33/00A23V2002/00A23V2200/306A23V2200/302A23V2200/30A23V2250/204
Inventor 帕尔哈提·柔孜排则丽耶·阿不都热衣木吾古力尼沙·麦麦提托合提杨晓君古丽米热·阿巴拜克日马生军邓杰阿卜杜瓦哈普·伊米提尼格尔热依·亚迪卡尔古扎努尔·艾斯卡尔
Owner XINJIANG AGRI UNIV
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