A kind of Pichia monteriti and its application in biological deodorization
A biological deodorization and yeast technology, applied in the biological field, can solve the problems of high equipment cost, secondary pollution, and high operation requirements, and achieve the effect of no biological hazard, high deodorization efficiency, and obvious degradation effect
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Embodiment 1
[0031] Example 1 Screening and identification of Pichia mongolica
[0032] 1. Pick out bones, napkins and other substances from the kitchen waste in Yuxiu Canteen of Zhejiang University of Technology, and then use a crusher to crush the remaining waste.
[0033] 2. Take 1g of crushed food waste and dilute it to 1×10 with ultrapure water -6 , 1×10 -7 , 1×10 -8 , and take 200 μL of each dilution to solid YPD medium, spread it evenly with a spreader stick, place it in a constant temperature incubator at 28°C for 48h to 72h, and pick a single colony for streaking and purification;
[0034] The formula of YPD medium is: yeast powder 10g, peptone 20g, glucose 20g, agar 15g.
[0035] 3. After purifying each single colony, pick out the strains that are pure white in color, smooth and shiny in shape, without wrinkles, and have a slight wine smell, and use the FastDNA Spin Kit for Soil kit to extract the genome from the single colony.
[0036] 4. Use the extracted genome as a templa...
Embodiment 2
[0038] The preparation of embodiment 2 biological deodorizing bacteria agents
[0039] 1. Insert the Pichia mongoliana strains screened in Example 1 into the YPD medium.
[0040] 2. Inoculate the activated bacteria into the YPD liquid medium for shaking culture (37°C, 180r / min) for more than 12 hours.
[0041] 3. Count the cultured bacterial solution and adjust the strain content to 10 8 ~10 9 CFU / ml.
[0042] 4. Centrifuge the bacterial solution (12000r / min, 10min), discard the supernatant after centrifugation, and collect the bacterial cells.
[0043] 5. Mix the bacterial cells with diatomaceous earth and corn stalks at a ratio of 1:1 (w / w), and then place them in a 28°C incubator until they are dry and constant weight, which are various bacterial agents. Diatomaceous earth is a molecular-grade product purchased in the market; corn stalks are pre-treated and then mixed with bacterial agents. The pretreatment steps are: firstly crush the raw materials of corn stalks into ...
Embodiment 3
[0048] Example 3 Carry out airtight deodorization test with kitchen waste as substrate
[0049] The fresh kitchen waste was crushed with a blender and divided into 500ml Erlenmeyer flasks. Each Erlenmeyer flask was divided into 100g (wet weight) of fresh food waste. Three parallel experiments were set up for each group of experiments. Then the bacterial agent prepared in Example 2 was added in an amount of 10g / bottle, and a blank control was set simultaneously. Then seal it with three layers of plastic wrap, and tie the mouth of the bottle tightly with a rubber band. Place in an incubator at 28°C, and measure the concentrations of ammonia and hydrogen sulfide after 24 hours.
[0050] After three groups of parallel experiments, it was found that the degradation rate of hydrogen sulfide in kitchen waste was 79.23%, and the degradation rate of ammonia gas was 25.71% after inoculation of biological deodorizing bacteria agent for 24 hours. From the above experimental results, it ...
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