Cicer arietinum saponin microcapsule as well as preparation method and application thereof

A technology of glycoside microcapsules and chickpeas, which is applied in the field of microcapsule preparation, can solve the problems of unseen chickpea protein, poor thermal stability, low entrapment rate and the like, and achieves good sustained release, thermal stability and stability. Good, high encapsulation rate

Active Publication Date: 2021-01-15
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, proteins currently used as wall materials, such as soybean protein, have the problems of easy allergies, poor thermal stability, and low embedding rate, which limit the use of proteins in microencapsulation technology.
[0005] There is no report of chickpea protein as wall material

Method used

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  • Cicer arietinum saponin microcapsule as well as preparation method and application thereof
  • Cicer arietinum saponin microcapsule as well as preparation method and application thereof
  • Cicer arietinum saponin microcapsule as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1, prepare chickpea protein by alkali-dissolving and acid-precipitating method

[0075] Weigh 10g of defatted chickpea flour, add 100mL of distilled water, adjust the pH to 9.0 with 1mol / L NaOH, stir at 500rpm at room temperature for 45min, centrifuge at 4°C and 5000rpm for 20min, collect the supernatant, repeat the above operation, and combine the supernatant solution, use 0.1mol / L HCl to adjust the pH to 4.5, acid precipitation for 1h, centrifuge at 4500g, 4°C for 20min, wash the precipitate with 4°C distilled water, resuspend the precipitate in water, adjust the pH to 7 with 1mol / L NaOH, Freeze-dried to obtain protein powder (chickpea protein).

Embodiment 2

[0076] Embodiment 2, adopt ultrasonic method to modify chickpea protein

[0077] Weigh 1.0 g of the chickpea protein prepared in Example 1, dissolve it in 15 mL of distilled water, stir for 2 min with a high-speed homogenizer at 10,000 rpm, ultrasonicate at room temperature for 30 min at a power of 140 W, and freeze-dry to obtain ultrasonically denatured protein.

Embodiment 3

[0078] Example 3. Preparation of Chickpea Saponin Extract by Ultrasound-Assisted Ethanol Extraction

[0079] Weigh 1.0 g of defatted chickpea flour, add 30 mL of 68% ethanol, and ultrasonicate for 45 min at an ultrasonic temperature of 50 °C and an ultrasonic power of 107 W, filter to obtain the ethanol extract, concentrate the ethanol extract to dryness under reduced pressure, and wash out with 10 mL of distilled water (Dissolve the dry matter after concentration under reduced pressure in distilled water), transfer to a separatory funnel, extract 30 min according to water phase: water saturated n-butanol (volume ratio) = 1:3, 3 times in total, and store in normal The total saponins in the butanol phase were concentrated to dryness under reduced pressure to obtain a crude chickpea saponin extract. The extraction rate of total chickpea saponins is 0.4823%.

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Abstract

The invention provides a cicer arietinum saponin microcapsule as well as a preparation method and application thereof, and belongs to the field of microcapsule preparation in a natural product processing technology. Firstly, a wall material for preparing the microcapsule is provided, and components of the wall material comprise denatured cicer arietinum protein. The invention further provides thecicer arietinum saponin microcapsule prepared from the wall material. Under the specific preparation method disclosed by the invention, the total saponin in the cicer arietinum saponin extract is highin extraction rate and high in purity. The cicer arietinum saponin microcapsule prepared from the specific wall material is high in drug loading capacity and good in embedding effect. Besides, the cicer arietinum saponin microcapsule disclosed by the invention has good slow release and thermal stability. In addition, by improving in-vivo antioxidant enzyme activity of diabetic mice, the cicer arietinum saponin microcapsule can achieve the effects of relieving oxidative stress injury of tissues such as liver and pancreas, improving insulin resistance state and enhancing insulin sensitivity, the blood glucose level of the diabetic mice can be effectively reduced, lipid metabolism disorder of the mice can be improved, and the cicer arietinum saponin microcapsule has an excellent blood glucose lowering effect and a wide application prospect.

Description

technical field [0001] The invention belongs to the field of microcapsule preparation in natural product processing technology, and in particular relates to a chickpea saponin microcapsule and its preparation method and application. Background technique [0002] Chickpea (Cicer arietinum L.) is named chickpea because it is shaped like a chick beak. It is second only to soybeans in the world. Mulei County, Xinjiang is the largest chickpea planting base in my country, with a planting area of ​​about 120,000 mu. Chickpeas are rich in protein, unsaturated fatty acids, minerals, dietary fiber, etc., and have high nutritional value. "Uyghur Medicine Records" records that chickpea has the functions of "clearing abnormal body fluids, opening body fluid blockages, and regulating the body", and is used for the treatment of diseases such as emaciation, loss of appetite, skin itching, and diabetes. Traditional Chinese medicine believes that chickpeas are "sweet in taste, flat in nature...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/42A61K47/36A61K36/48A61P3/10
CPCA61K9/5052A61K9/5036A61K36/48A61P3/10A61K2236/333A61K2236/55A61K2236/51
Inventor 江岩黄煦杰王德萍
Owner CHENGDU MEDICAL COLLEGE
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