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Fresh elm leaf aqueous extract powder as well as preparation, antithrombotic effect and application thereof

A technology of elm leaves and water extracts, which is applied in the field of biomedicine and can solve the problems of ignorance of the biological activity of all components

Inactive Publication Date: 2021-01-15
北京恒润泰生医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the biological activity of a single component of fresh elm leaf aqueous extract powder has been reported, the combined biological activity of all components is unknown

Method used

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  • Fresh elm leaf aqueous extract powder as well as preparation, antithrombotic effect and application thereof
  • Fresh elm leaf aqueous extract powder as well as preparation, antithrombotic effect and application thereof
  • Fresh elm leaf aqueous extract powder as well as preparation, antithrombotic effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 prepares fresh elm leaf aqueous extract powder

[0047] Wash fresh elm leaves with tap water, take 300g and heat in 900mL distilled water at 60°C-90°C for 2 hours while stirring (220rpm / min). Cool to room temperature, filter, and concentrate under reduced pressure to obtain 36 g of fresh elm leaf water extract powder.

Embodiment 2

[0048] Embodiment 2 measures the chromatogram and mass spectrometry ion flow spectrum of fresh elm leaf aqueous extract powder

[0049] 2-1. Preparation of sample solution (10mg / mL)

[0050] Weigh 26.7mg fresh elm leaf water extract powder and dissolve the powder in 2.67mL ultrapure water. The obtained solution was ultrasonically shaken for 1 minute, and then centrifuged at 13000r / min for 10 minutes. Take the supernatant, pass through a 0.22μm filter membrane, and place it in a sample bottle for chromatography and mass spectrometry.

[0051] 2-2. Chromatographic conditions

[0052] Chromatographic conditions

[0053] Chromatographic column: Waters, Acquity T3 column (2.1×100mm i.d., 1.7μm);

[0054] Injection volume: 2μL;

[0055] PDA detector: 190-400nm;

[0056] Mobile phase: water (0.1% formic acid), acetonitrile;

[0057] The mobile phase washes the column according to the gradient in Table 1.

[0058] Table 1 Mobile phase gradient table

[0059]

[0060] ...

Embodiment 3

[0067] Embodiment 3 specifies the structure of 31 components in the fresh elm leaf aqueous extract powder

[0068] The UPLC chromatogram of Example 2 was coupled with mass spectrometry, and the UPLC-mass spectrometry of the fresh elm leaf water extract powder was determined. The mass spectrometry conditions are two modes of electrospray ionization, positive ion and negative ion. Ion mode parameters: capillary voltage is 1000V, desolvation gas flow rate is 800L / h, temperature is 450°C, source temperature is 120°C, cone gas flow rate is 50L / h, spray pressure is 6bar, fragmentation voltage is 20-45V, The sampling cone voltage is 6V, the acquisition mode is MSE continuum resolution mode, the data acquisition range of the mass number to charge number ratio (m / z) of charged particles is 100-1500, the Trap fragmentation voltage of the low energy channel is 6V, and the high energy The channel Trap fragmentation voltage selection gradient voltage is 20-60V, and LE (leucine enkephalin)...

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Abstract

The invention discloses fresh elm leaf aqueous extract powder, a preparation method of the fresh elm leaf aqueous extract powder and an effect of the fresh elm leaf aqueous extract powder in resistingvenous thrombosis and arterial thrombosis. The fresh elm leaf aqueous extract powder prepared by the method provided by the invention contains 17 quinic acid compounds and 14 quercetin compounds. Experiments prove that the fresh elm leaf aqueous extract powder disclosed by the invention can effectively inhibit the venous thrombosis and the arterial thrombosis, so that the invention discloses application of the fresh elm leaf aqueous extract powder in preparation of anti-venous thrombosis and anti-arterial thrombosis medicines. The invention provides an effective technical means for thrombus treatment.

Description

technical field [0001] The invention relates to a method for preparing fresh elm leaf water extract powder, and relates to the application of the fresh ulm leaf water extract powder prepared by the method in the preparation of anti-venous thrombosis and anti-arterial thrombosis drugs. The invention belongs to the field of biomedicine. Background technique [0002] Venous thrombosis has become one of the diseases with high morbidity and mortality. The most serious venous thrombosis is mainly cerebral venous thrombosis and lower extremity deep venous thrombosis. Cerebral venous thrombosis originates from dural venous sinus thrombosis and / or cerebral venous texture thrombosis, which can lead to cerebral venous blockage, increased intracranial pressure, cerebral ischemia or intracranial hemorrhage. The standard treatment strategy for cerebral venous thrombosis is oral anticoagulation. The incidence of upper / lower extremity deep vein thrombosis and pulmonary embolism is 0.1-0....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K9/14A61P7/02A61K127/00
CPCA61K9/14A61K36/185A61K2236/331A61P7/02
Inventor 彭师奇赵明黄飞
Owner 北京恒润泰生医药科技有限公司
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