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Composite microbial preparation for degrading pig manure indole

A technology of compound microorganisms and pig manure, applied in microorganisms, microorganism-based methods, biological sludge treatment and other directions, can solve the problems of difficult preservation of strains, increase feces odor, harm the environment, etc. The effect of avoiding the waste of bacteria and helping to purify the environment

Inactive Publication Date: 2021-01-15
XINJIANG XIPU BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pig manure contains a large amount of harmful components such as nitrogen, phosphorus, and organic pollutants, which will cause various pollutions such as gas pollution, microbial pollution, and soil pollution, and seriously endanger the environment.
[0003] In recent years, as people's awareness of environmental protection has gradually increased, a variety of feces treatment agents and purification devices have appeared on the market. Gas is processed, and the presence of indole greatly increases the odor of feces. However, there are few relevant studies on how to decompose indole in feces in the prior art.
[0004] Chinese patent application CN104628453A discloses a deodorizing and decomposing agent for livestock and poultry manure and a method for treating feces. Although the deodorizing and decomposing agent can handle feces more conveniently and has the effect of reducing insect eggs and germ pollution, however, This decomposing deodorant still cannot effectively remove indole, and various substances are added to the feces treatment, which is likely to cause secondary pollution to the environment
[0005] In the article "Development of Composite Microbial Preparations and Degradation of Indole in Pig Manure" published in the Journal of Animal Science in November 2019, a method for improving the degradation rate of indole by mixing four strains is given , however, the degradation method provided by it only contains four strains, which can only degrade indole, and will not change the harm that feces have brought to the environment. In the process, the strains will not be directly added to the feces, and the general overfermentation of these strains will also affect the environment. Therefore, the indole degradation mentioned in the article has an application value in the actual treatment of indole in feces. not high
[0006] To sum up, in the prior art, the degradation rate of indole in feces is generally low, the practicability is poor, and it is easy to cause secondary pollution to the environment and other hazards.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 A kind of compound microorganism preparation of degrading pig feces indole

[0030] The complex microbial preparation for degrading pig feces indole comprises the following components and their mass percentages: 1% corncob powder, 2% Hovenia dulcis fruit extract, 2% yucca extract, 1% fragrance agent, and complex microbial bacteria 8% seed, 86% water; the fragrance agent is composed of peppermint oil and sage extract in a mass ratio of 10:2; The composition of Nocardia-like bacteria ranges from 7:5:12:3. The Streptomyces luteus, Lactobacillus casei and Nocardioides albicans are cultivated with a solid fermentation medium; the Bacillus subtilis is cultivated with a Bacillus medium; The number of bacteria reaches 2.5×10 8 ; Described Lactobacillus casei is cultivated to reach 1.2 * 10 bacteria counts in every gram of culture medium 8 ; The bacillus subtilis is cultivated until the number of bacteria per gram of culture medium reaches 9.3 × 10 7 ; The albic...

Embodiment 2

[0034] Embodiment 2 A kind of compound microorganism preparation of degrading pig feces indole

[0035] The compound microbial preparation comprises the following components and their mass percentages: 3% corncob powder, 5% Hovenia dulcis fruit extract, 3% yucca extract, 2% fragrance agent, 10% compound microbial strains, 77% water %; the fragrance agent is composed of peppermint oil and sage extract in a mass ratio of 15:5; The bacteria number range ratio is 9:8:15:5; the Streptomyces flavinus, Lactobacillus casei and Nocardioid albicans are cultivated with a solid fermentation medium; the Bacillus subtilis is cultivated with a Bacillus medium; the The above-mentioned Streptomyces flavinus was cultured until the number of bacteria in each gram of culture medium reached 2.8×10 8 ; Described Lactobacillus casei is cultivated to reach 1.5 * 10 bacteria counts in every gram of culture medium 8 ; The bacillus subtilis is cultivated to reach 9.6 * 10 per gram of culture medium. ...

Embodiment 3

[0039] Embodiment 3 A kind of compound microorganism preparation of degrading pig feces indole

[0040] The compound microbial preparation consists of the following components and their mass percentages: 2% corncob powder, 4% Hovenia dulcis fruit extract, 2.4% yucca extract, 1.5% fragrance agent, 9.2% compound microbial strains, water 80.9%; the fragrance agent is composed of peppermint oil and sage extract in a mass ratio of 13:4; the composite microbial strain is composed of Streptomyces luteus, Lactobacillus casei, Bacillus subtilis and Nocardioid albicans Composed according to the ratio of the number of bacteria to 8:7:13:4; the Streptomyces flavinus, Lactobacillus casei and Nocardioides albicans are cultivated with solid fermentation medium; the Bacillus subtilis is cultivated with Bacillus medium; The Streptomyces flavinus is cultivated until the number of bacteria per gram of culture medium reaches 2.7×10 8 ; Described Lactobacillus casei is cultivated to reach 1.3 * 1...

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PUM

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Abstract

The invention belongs to the technical field of livestock and poultry excrement treatment, and particularly relates to a composite microbial preparation for degrading pig excrement indole. The composite microbial preparation provided by the invention comprises composite microbial strains, a flavoring agent, corncob powder, hovenia dulcis thunb extract and other components. The composite microbialpreparation provided by the invention can effectively degrade indole in pig manure, has a degradation rate of 75% or above, can effectively remove peculiar smell, does not contain any harmful substance, and does not cause secondary pollution to the environment while keeping air fresh.

Description

technical field [0001] The invention belongs to the technical field of excrement treatment of livestock and poultry, and in particular relates to a composite microbial preparation for degrading pig feces indole. Background technique [0002] According to incomplete statistics, there are about 500 million live pigs in China, one for every three people on average. Judging from the total figures, like China's population, the number of live pigs in China is much higher than that of other countries. Assuming that each pig produces 2.5kg of manure per day, all the pigs in China together produce at least 1 million tons of manure. However, pig manure contains a large amount of harmful components such as nitrogen, phosphorus, and organic pollutants, which will cause various pollutions such as gas pollution, microbial pollution, and soil pollution, and seriously endanger the environment. [0003] In recent years, as people's awareness of environmental protection has gradually increas...

Claims

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Application Information

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IPC IPC(8): C02F11/02C12N1/20C12R1/465C12R1/245C12R1/125C12R1/365
CPCC02F11/02C12N1/20
Inventor 余忠丽恽辉王俊青赵大伟
Owner XINJIANG XIPU BIOLOGICAL SCI & TECH
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