Targeted oxygen-carrying nano-enzyme preparation and preparation method thereof
A technology of nano-enzyme and enzyme preparation, applied in the field of nano-medicine, can solve the problems of complex preparation process, unstable properties, lack of nanoparticles, etc., and achieve the effect of easy operation and popularization, and simple and easy preparation method.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0041] The preparation method of the protease hybrid nano-enzyme preparation coated with oxygen-loaded ferrous porphyrin of the present invention, the specific steps are:
[0042] (1) Preparation of ferrous porphyrin; the ferrous porphyrin is specifically prepared by reacting ferric porphyrin and ascorbic acid at room temperature under the protection of argon or nitrogen.
[0043](2) Prepare reduced human serum albumin; the reduced human serum albumin is specifically dissolved in water using human serum albumin and reduced glutathione, and placed in a dialysis bag after reacting in a shaker at room temperature Prepared by dialysis.
[0044] (3) Mix reduced human serum albumin and an enzyme preparation in water, adjust the pH value of the solution to 7-8, and the enzyme preparation is collagenase or hyaluronidase.
[0045] (4) Take the reduced ferrous porphyrin, stir it in water, and add it into (3).
[0046] (5) Add methanol or ethanol solution, stir vigorously, and dialyze ...
Embodiment 1
[0049] The preparation method of embodiment 1 ferrous porphyrin
[0050] Concrete preparation method: mix porphyrin (purchased by Sigma-Aldrich, CAS: 69458-20-4) and metal ferrous salt in equimolar ratio in anhydrous DMF solution, and carry out ferrous ion coordination under argon protection , the UV-Vis absorption spectrometer monitors the progress of the reaction. After the reaction solution turns from yellow to dark red, and the Q band peaks change from 4 to 2 with a certain degree of red shift, the reaction stops, cools to room temperature, and places In an ice-water bath, keep away from light overnight; wash the collected filter cake with deionized water, and dry it in vacuum. Then it was separated and purified by column chromatography, using silica gel as the stationary phase, and dichloromethane and methanol at a volume ratio of 3:1 as the mobile phase for separation and purification. Rotary evaporation at low temperature and drying to obtain a purified ferrous porphyr...
Embodiment 2
[0051] Embodiment 2 The preparation method of targeted oxygen-carrying nanozyme preparation
[0052] Specifically include the following steps:
[0053] (1) Iron porphyrin was dissolved in DMSO at a concentration of 5 mg / mL. Ascorbic acid was dissolved in DMSO at a concentration of 2.5 mg / mL. Under the protection of argon, it was reacted at room temperature for 5 hours to obtain ferrous porphyrin (Fe (II)TP), standby;
[0054] (2) Human serum albumin and reduced glutathione were dissolved in 2 mL of deionized water at a molar ratio of 1:1, and reacted in a shaker at room temperature for 30 minutes at a speed of 100 rpm. After the reaction, placed in a 3.5KDa dialysis bag medium, dialyzed overnight to obtain reduced human serum albumin (SH-HSA), and set aside;
[0055] (3) Weigh 100mg of reduced human serum albumin and 10mg of collagenase in 10mL of deionized water, with NaHCO 3 Adjust the pH of the solution to 7;
[0056] (4) Pipette 1 mL of reduced Fe(II)TP, disperse in 1 ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Electric potential | aaaaa | aaaaa |
| Particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More