Application of protein and related biological materials thereof to regulation and control on mechanical strength of plants

A technology of mechanical strength and biological materials, applied in the fields of plant peptides, plant products, plant genetic improvement, etc., can solve the problems of difficult degradation of cell wall components, affecting crop rooting, low recycling efficiency, etc., to achieve high recycling efficiency.

Active Publication Date: 2021-01-22
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, limited by the difficulty in the degradation of cell wall components, the recycling efficiency of crop straw in my country is still very low.
Straw returning directly to the field will affect the rooting of the next crop, while field burning not only wastes resources, but also causes huge environmental pollution

Method used

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  • Application of protein and related biological materials thereof to regulation and control on mechanical strength of plants
  • Application of protein and related biological materials thereof to regulation and control on mechanical strength of plants
  • Application of protein and related biological materials thereof to regulation and control on mechanical strength of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1 Discovery of rice brittle stalk and brittle leaf genes

[0072] 1. Acquisition of rice crisp stem and crisp leaf mutant bcl1

[0073] Rice brittle culm and leaf mutant brittle culm and leaf 1 (abbreviated as bcl1 or mutant bcl1) is a spontaneous mutation material in the tissue culture process of rice TP309.

[0074] 2. Phenotypic identification of rice brittle stem and brittle leaf mutant bcl1

[0075] Compared with rice TP309, mutant bcl1 mainly exhibits:

[0076] 1. Stems ( figure 1 shown): Compared with the stalks of rice TP309 (represented by "TP309" in the figure), the stalks of the mutant bcl1 (represented by "bcl1" in the figure) were brittle and the secondary cell walls of the sclerenchyma cells were Thin;

[0077] 2. Blades ( figure 2 Shown): Compared with the leaves of rice TP309 (represented by "TP309" in the figure), the leaves of the mutant bcl1 (represented by "bcl1" in the figure) became brittle and the secondary cell walls of the scle...

Embodiment 2

[0082] Example 2 Functional verification of BCL1 gene

[0083] 1. Acquisition of Complementary Vector Transgenic Plants

[0084] In order to verify the role of BCL1 in the regulation of rice stem and leaf fragility, functional verification was carried out by BCL1 genome complementation experiments in mutant bcl1.

[0085] 1) Construction of complementary vectors

[0086] 1. Genomic DNA of two-week-old seedlings of rice TP309 was extracted.

[0087] 2. Using the DNA in step 1 as a template, perform PCR amplification with a primer pair composed of BCL1-com1-F and BCL1-com1-R, and obtain a size of 5302 bp as shown in the 1-5302 position of sequence 1 in the sequence table The amplified product fragment a (SEQ forward direction).

[0088] BCL1-com1-F: 5′-CACTCCATACATTGCGGGGT-3′

[0089] BCL1-com1-R: 5′-ACATTCTTCCCGCTTTCGGT-3′

[0090] 3. Using fragment a in step 2 as a template, carry out PCR amplification with a primer pair composed of BCL1-com2-F and BCL1-com2-R (the underl...

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Abstract

The invention discloses application of a protein and related biological materials thereof to regulation and control on mechanical strength of plants. The invention firstly discloses application of a BCL1 protein and related biological materials thereof to regulation and control on mechanical strength of plants. The invention further discloses a method for cultivating transgenic plants of which stalks and / or leaves have increased brittleness. The invention not only provides the theoretical basis for cultivating fragile stalk and fragile leaf plant materials and rice varieties of which cell wallcomponents are easy to degrade and which is high in recycling efficiency, but also provides a new gene resource for rice breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the use of protein and related biological materials in regulating the mechanical strength of plants. Background technique [0002] The thickness and composition of the cell wall are key elements in determining the mechanical strength of plant tissues. Mechanical strength is an important agronomic trait of crops, which is closely related to crop lodging resistance, resistance to pathogenic bacteria and yield. Structurally, the plant cell wall includes intercellular layer, primary wall and secondary wall, which is a complex network structure formed in a certain sequence during the growth and development of cells. The intercellular layer is a special structure formed between two adjacent cells during the process of cell division to produce new cells. The main component is pectin. squeeze. The primary wall is located inside the intercellular layer and is mainly composed of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H5/10A01H6/46
CPCC07K14/415C12N15/8213C12N15/8218C12N15/8261
Inventor 翟文学李春荣刘鹏程江光怀
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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