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Application of oyster mushroom polysaccharide selenoside-III anticancer active component in preparation of anti-gastric cancer drug

A technology of oyster mushroom polysaccharide and anti-cancer activity, applied in the field of medicine, can solve the problems of less than 30% survival rate, tumor metastasis and spread, large damage, etc., and achieve the effects of excellent anti-cancer activity and good anti-cancer activity

Active Publication Date: 2021-01-29
湖南万臻生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The treatment strategies for patients with advanced gastric cancer mainly include surgical resection, chemotherapy, radiotherapy and other treatment measures, but the prognosis is still poor, and the five-year survival rate is less than 30%
This is mainly due to the fact that the existing treatment methods have caused great damage to the normal tissues of the body, and the tumor is prone to metastasis and spread.

Method used

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  • Application of oyster mushroom polysaccharide selenoside-III anticancer active component in preparation of anti-gastric cancer drug
  • Application of oyster mushroom polysaccharide selenoside-III anticancer active component in preparation of anti-gastric cancer drug
  • Application of oyster mushroom polysaccharide selenoside-III anticancer active component in preparation of anti-gastric cancer drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] (1) Preparation of crude selenium polysaccharide

[0060] Take 15 g of selenium-enriched Pleurotus ostreatus powder (provided by Hunan Wanzhen Biotechnology Co., Ltd.) into a round bottom flask, add 120 mL of 80% ethanol-water solution, stir and reflux at 65° C. for 3 h, filter with suction to obtain a filter cake, and dry.

[0061] Water extraction: Take 10 g of the filter cake in a flask, add 300 mL of ultrapure water, stir at 85° C. for 3 h, centrifuge, and concentrate the supernatant to obtain a viscous liquid.

[0062] Alcohol precipitation: drop 4 times the amount of absolute ethanol into the viscous liquid, place it at 4°C for 24 hours, and centrifuge to obtain a precipitate.

[0063] Deproteinization: dissolve the precipitate in 100mL of water, add 1 / 4 volume of sevage reagent, centrifuge to get the supernatant, repeat 6 times, dialyze with a dialysis bag, freeze-dry to obtain crude polysaccharide (also known as Pleurotus ostreatus crude polysaccharide) or crud...

Embodiment 2

[0074] Pharmacodynamic experiment of polysaccharide selenoside-Ⅲ (obtained in Example 1) on human gastric cancer cells

[0075] When the MGC803 cell density reaches 80-90%, digest with trypsin until the cells become round, and then add cell culture medium to stop the digestion. The cell suspension was appropriately diluted and counted with a hemocytometer, and the plate (96-well plate) was seeded at a density of 8000 cells per well, and the 96-well plate was placed in a cell incubator and incubated for 24 hours.

[0076] Afterwards, the culture medium in the 96-well plate was replaced with serum-free cell culture medium, and incubated in a cell culture incubator for 24 hours.

[0077] The previous serum-free medium was replaced with cell culture medium containing different concentrations of polysaccharide selenoside-III (0 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, 400 μg / mL, 600 μg / mL) and incubated for 24 h. And the cell-free and simple cell culture medium was used as the negat...

Embodiment 3

[0093] Drug effect test of polysaccharide selenoside-Ⅲ (embodiment 1) on normal cells

[0094] When the density of normal human liver cells reaches 80-90%, digest with trypsin until the cells become round, and then add cell culture medium to stop the digestion. The cell suspension was appropriately diluted and counted with a hemocytometer, and the plate (96-well plate) was seeded at a density of 8000 cells per well, and the 96-well plate was placed in a cell incubator and incubated for 24 hours.

[0095] Afterwards, the culture medium in the 96-well plate was replaced with serum-free cell culture medium, and incubated in a cell culture incubator for 24 hours.

[0096] The previous serum-free medium was replaced with cell culture medium containing different concentrations of polysaccharide selenoside-III (0 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, 400 μg / mL, 600 μg / mL) and incubated for 24 h. And the cell-free and simple cell culture medium was used as the negative control group...

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Abstract

The invention belongs to the technical field of medicine development and particularly discloses pleurotus ostreatus polysaccharide selenoside III. The invention discloses an oyster mushroom polysaccharide selenoside III anticancer drug capable of effectively inhibiting gastric cancer, and discloses a preparation method of the oyster mushroom polysaccharide selenoside III anticancer drug. The oyster mushroom polysaccharide selenoside III anticancer drug can specifically kill gastric cancer cells, and can promote metabolism and proliferation of normal cells; a feasible scheme is provided for treating gastric cancer.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an active ingredient of oyster mushroom polysaccharide selenoside-III that can promote the proliferation and growth of normal cells and efficiently inhibit gastric cancer cells. [0002] technical background [0003] Gastric cancer is currently the second most common malignant tumor in the world, and its mortality rate ranks third. Gastric cancer accounts for nearly half of the patients in China. In the past two decades, with the development of my country's social economy and the change of people's eating habits, the incidence of gastric cancer has increased year by year, which has seriously threatened the physical and mental health of human beings. It is urgent to study anticancer drugs with specific effects on gastric cancer. [0004] Due to the low early detection rate of gastric cancer, most of them have reached the advanced stage of gastric cancer during treatment. The tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/04A61K31/715A61P35/00C08B37/00
CPCA61K33/04A61K31/715A61P35/00C08B37/0003C08B37/006A61K2300/00Y02A50/30
Inventor 钟世安王德刘慧张云山张卓敏沈剑
Owner 湖南万臻生物科技有限公司
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