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Collagen lamellar matrix material, preparation method and application thereof

A technology of matrix material and collagen layer, which is used in medical science, prosthesis, tissue regeneration, etc., can solve the problems of poor biocompatibility of artificial synthetic materials, unable to meet the requirements of surgical suture, affecting transparency and mechanical strength, etc. Or the effect of restoring vision, excellent biocompatibility, and good mechanical properties

Inactive Publication Date: 2021-02-02
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the carrier scaffold materials used for tissue engineering artificial cornea mainly include heterogeneous decellularized cornea, natural biomaterials (including collagen, gelatin, chitosan, fibrin, silk fibroin, amniotic membrane, etc.), artificial synthetic materials (including polyethylene Alcohol, polylactic acid, polyglycolic acid, etc.), but there are certain shortcomings and deficiencies, has not yet developed a material that can meet the requirements of clinical corneal implant therapy
For example, the decellularized detergent in the preparation process of xenogeneic (pig and other animal sources) decellularized corneal stroma will destroy the original structure and composition of the matrix, affect its transparency and mechanical strength, and may also cause inflammation after transplantation; while collagen, shell Scaffold materials made of natural biological materials such as polysaccharides and fibrin have too fast degradation rate and poor mechanical strength, which cannot meet the requirements of surgical suture; The toxic effect of
The use of cell sheet engineering technology does not require the use of scaffold materials, and the use of cell sheet engineering technology to reconstruct the cornea can overcome these shortcomings, but it is difficult to obtain seed cells when this technology is used for tissue reconstruction, and the superposition of multiple cell sheets will lead to internal cell necrosis. There are still many major problems

Method used

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  • Collagen lamellar matrix material, preparation method and application thereof
  • Collagen lamellar matrix material, preparation method and application thereof
  • Collagen lamellar matrix material, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] A preferred embodiment of the present invention provides a method for preparing a collagen lamellar matrix material, the specific steps are as follows:

[0040] Spread the decalcified fish scales on the bottom plate with holes, each hole of the bottom plate corresponds to a sample, start the vacuum device to make the samples adsorb on the bottom plate, and select a partition with a suitable pore size to spread on the fish scale sample as required. Spray the etchant evenly on the surface of the decalcified fish scales and let it stand. At regular intervals, spray cleaning solution to wash off the etching solution on the surface of fish scales, and then spray new etching solution again. Different partitions were replaced during the etching process to ensure that the etching time for the middle part of the sample reached 17 hours, and the etching time for the edge part reached 8 hours. After the etching is completed, rinse with ultrapure water for 3 times, each time for 1...

Embodiment 2

[0047] A preferred embodiment of the present invention provides a method for preparing a collagen lamellar matrix material, the specific steps are as follows:

[0048] Spread the decalcified fish scales on the bottom plate with holes, each hole of the bottom plate corresponds to a sample, start the vacuum device to make the samples adsorb on the bottom plate, and select a partition with a suitable pore size to spread on the fish scale sample as required. Spray the etchant evenly on the surface of the decalcified fish scales and let it stand. At regular intervals, spray cleaning solution to wash off the etching solution on the surface of fish scales, and then spray new etching solution again. Different partitions were replaced during the etching process to ensure that the etching time for the middle part of the sample reached 18 hours, and the etching time for the edge part reached 9 hours. After the etching is completed, rinse with ultrapure water for 3 times, each time for 1...

Embodiment 3

[0053] A preferred embodiment of the present invention provides a method for preparing a collagen lamellar matrix material, the specific steps are as follows:

[0054] Spread the decalcified fish scales on the bottom plate with holes, each hole of the bottom plate corresponds to a sample, start the vacuum device to make the samples adsorb on the bottom plate, and select a partition with a suitable pore size to spread on the fish scale sample as required. Spray the etchant evenly on the surface of the decalcified fish scales and let it stand. At regular intervals, spray cleaning solution to wash off the etching solution on the surface of fish scales, and then spray new etching solution again. Different partitions were replaced during the etching process to ensure that the etching time for the middle part of the sample reached 19 hours, and the etching time for the edge part reached 8 hours. After the etching is completed, rinse with ultrapure water for 3 times, each time for 1...

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Abstract

The invention discloses a collagen lamellar matrix material, a preparation method and application thereof. The preparation method comprises the following steps: etching and rinsing the surface of a collagen sheet obtained after pretreatment such as degreasing, decalcification and decellularization, then placing the collagen sheet in a cross-linking mold for cross-linking, then carrying out cleaning, and finally carrying out sterilization treatment. The component structure of the prepared collagen lamellar matrix material is similar to that of a natural cornea, and the collagen lamellar matrixmaterial has excellent optical properties, mechanical properties and good cell compatibility, has great significance in in-vitro cornea reconstruction, and is expected to become one of ideal options of cornea substitutes.

Description

technical field [0001] The invention belongs to the technical field of biological materials, tissue engineering, artificial organs and medical devices, and in particular relates to a collagen lamellar matrix material and its preparation method and application. Background technique [0002] The cornea is located at the front of the eyeball and is the protective window of the eye. It protects the fragile intraocular tissues by bearing intraocular pressure and preventing damage from external factors. The cornea is a highly structured transparent tissue bordered by an opaque sclera composed of dense, interlaced fibers. The cornea is the main refractive organ in the eyeball. It has the functions of light transmission and refraction, and plays an important role in the formation of vision. The diameter of the human cornea is about 12mm, and the radius of the central front plane is about 7.8mm; the thickness of the cornea is different in different parts, the center is about 520μm, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/36A61L27/50
CPCA61L27/24A61L27/3604A61L27/3687A61L27/3691A61L27/50A61L2430/16C08L89/00
Inventor 蒋波冯欢欢尹越王宁馨郭纪彤
Owner SICHUAN UNIV
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