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Bacillus altitudinis with potassium-dissolving effect and use of bacillus altitudinis

A technology of Bacillus alpinus and potassium decomposing, which is applied in the direction of application, chemicals and bacteria for biological control, to achieve the effect of promoting germination, promoting growth and improving root length

Active Publication Date: 2021-02-05
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there have been no reports of Bacillus alpinus in dissolving potassium so far. Therefore, it is of great significance to study the activation ability and growth-promoting effect of Bacillus alpinus on potassium feldspar for the development of new potassium-dissolving microbial resources.

Method used

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  • Bacillus altitudinis with potassium-dissolving effect and use of bacillus altitudinis
  • Bacillus altitudinis with potassium-dissolving effect and use of bacillus altitudinis
  • Bacillus altitudinis with potassium-dissolving effect and use of bacillus altitudinis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The separation and purification of embodiment 1 YC602

[0053] (1) Separation

[0054] Prepare potassium soluble solid medium: glucose 5.0g, magnesium sulfate heptahydrate 0.5g, calcium carbonate 0.1g, ferric chloride 0.005g, tricalcium phosphate 2.0g, potassium feldspar 2.0g, agar 18.0g, distilled water 1L, pH 7.0-7.5.

[0055] Take the potato soil in Huizhou City, Guangdong Province as the screening soil sample, accurately weigh 10.0g of the soil sample, put it into a 250mL triangular flask containing 90mL of sterile water (with 5-7 glass beads), put it in a shaker, and Shake at 180rpm for 30min to fully disperse the soil sample, let it stand for 20-30s, take 5mL of the supernatant in a test tube, and heat at 90°C for 10min. Add 100 μL of the solution to a 2 mL centrifuge tube containing 900 μL sterile water to obtain 10 -2 solution, according to this method, sequentially obtain 10 -3 、10 -4 solution. Take 100 μL10 -4 The diluted solution was spread on the pota...

Embodiment 2

[0060] Example 2 Characterization of YC602

[0061] (1) Colony Morphological Characteristics

[0062] The bacterium colony formed after cultivating on nutrient agar medium for 24h is irregular shape, light yellow, smooth surface, not viscous and easy to provoke, and biofilm is produced.

[0063] (2) Growth characteristics

[0064] In LB liquid culture medium, under the conditions of rotation speed 180, temperature 37°C, and initial pH value 7.0, cultured for 18 hours, the number of viable bacteria measured was 3.2×10 7 cfu mL -1 .

[0065] (3) Gram staining

[0066] Pick a single colony of YC602 and culture it in liquid LB medium for 12 hours, pipette 10 μL of sterile water on the glass slide, pipette 5 μL of bacterial liquid into the added sterile water, mix well, dry and fix on the flame; Dye for 1 min, wash with water, and dry naturally; mordant with iodine solution for 1 min, wash with water, and dry; decolorize with 95% ethanol for 30 seconds, wash with water, and dr...

Embodiment 3

[0077] Example 3 The effect experiment of YC602 dissolving mineral potassium in the potassium solution liquid medium

[0078] (1) Medium preparation:

[0079] ①Potassium solution liquid medium: glucose 5.0g, magnesium sulfate heptahydrate 0.5g, calcium carbonate 0.1g, ferric chloride 0.005g, tricalcium phosphate 2.0g, potassium feldspar 2.0g, distilled water 1L, pH 7.0-7.5 .

[0080] ②LB liquid medium: peptone 10.0g, yeast extract powder 5.0g, sodium chloride 10.0g, distilled water 1L, pH7.0-7.5.

[0081] ③ LB solid medium: peptone 10.0g, yeast extract powder 5.0g, sodium chloride 10.0g, distilled water 1L, agar 18.0g, pH7.0-7.5.

[0082] (2) Preparation of test solution:

[0083] Pick the YC602 bacteria in glycerol and streak on the LB solid medium plate, and culture at 37°C for 12h. Put YC602 on the plate into a test tube containing LB liquid medium, culture at 37°C, 180rmp, on a shaker for 12h. Transfer the culture medium to fresh LB liquid medium, and shake to OD in a...

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Abstract

The present invention particularly discloses a bacillus altitudinis YC602 strain and use of the bacillus altitudinis YC602 strain. The preservation number of the bacillus altitudinis YC602 strain is GDMCC No.61221. The potassium-dissolving bacterium YC602 is separated from rhizosphere soil of potatoes in Huizhou city in Guangdong province. The bacillus altitudinis is preliminarily identified by adopting a 16SrRNA and a rpob sequence analysis method. The bacteria can dissolve insoluble potassium feldspar, improve germination of plant seeds and growth of root systems, and promote growth of plants. Besides, the strain has a function of producing auxin (IAA), has remarkable resistance to Cu (II), Cd (II), Zn (II) and Al (III) metal stress and acid stress, and has important significance in agricultural production.

Description

technical field [0001] The invention relates to the technical field of plant rhizosphere growth-promoting bacteria, and relates to a microorganism and its application, in particular to a strain of Bacillus altitudinis YC602 and its application. Background technique [0002] Potassium is one of the three essential nutrients for crop growth and development. Potassium deficiency will affect plant growth, root development, and resistance to diseases and insect pests. Although the content of potassium in the soil is rich, 98% of it exists in the form of minerals such as potassium feldspar and mica, and only the available potassium that does not exceed 2% of the total potassium in the soil is in the form that plants can directly absorb and utilize. Using microorganisms to dissolve mineral potassium in soil is an economical, effective and environmentally friendly measure to solve the problem of potassium deficiency in soil, which is of great significance to the development of ecolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C09K17/14A01N63/22A01N43/38A01P21/00B09C1/10C12R1/07C09K101/00
CPCC12N1/20C12N1/02C09K17/14A01N63/22A01N43/38B09C1/10C09K2101/00C12R2001/07C12N1/205
Inventor 蔡燕飞刘晓玉李福艳王玉琪任宗玲彭子倩
Owner SOUTH CHINA AGRI UNIV
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