Bacillus bataviensis BY08 and application thereof

A technology of bacillus, microbial strain, applied in the field of microbiology

Active Publication Date: 2020-10-27
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, so far, there have been no reports of Bacillus Batavia in terms of potassium solution, heavy metal stress, and antagonism agains...

Method used

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  • Bacillus bataviensis BY08 and application thereof
  • Bacillus bataviensis BY08 and application thereof
  • Bacillus bataviensis BY08 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Isolation and purification of embodiment 1 bacterial strain

[0041] (1) Separation

[0042] Preparation of potassium-soluble solid medium: glucose 5.0g, magnesium sulfate heptahydrate 0.5g, calcium carbonate 0.1g, ferric chloride 0.005g, tricalcium phosphate 2.0g, potassium feldspar 2.0g, agar 18.0g, distilled water 1L, pH 7.0-7.5.

[0043] Take the soil of Baiyun Mountain in Guangzhou City, Guangdong Province as the screening soil sample, accurately weigh 10.0g of the soil sample, put it into a 250mL triangular flask containing 90mL of sterile water (5-7 glass beads), 180rpm, shaker After 30 minutes, the soil samples were fully dispersed, and left to stand for 20-30 seconds, then 5 mL of the supernatant was taken in a test tube, and heated at 90°C for 10 minutes. Add 100 μL of the solution to a 2 mL centrifuge tube containing 900 μL sterile water to obtain 10 -2 solution, according to this method, sequentially obtain 10 -3 、10 -4 solution. Take 100 μL10 -4 The ...

Embodiment 2

[0048] Example 2 Characterization

[0049] (1) Colony Morphological Characteristics

[0050] The colonies formed after culturing on nutrient agar medium for 24 hours were irregular in shape, light yellow, moist and smooth on the surface, not sticky and easy to provoke; indole negative.

[0051] (2) Growth characteristics

[0052] In LB liquid culture medium, under the conditions of rotation speed 180rpm, temperature 37℃, and initial pH value 7.0, cultured for 18h, the number of viable bacteria measured was 3.2×10 7 cfu mL -1 .

[0053] (3) Gram staining

[0054] Pick a single colony of BY08 and culture it in liquid LB medium for 12 hours, pipette 10 μL of sterile water on the glass slide, pipette 5 μL of bacterial liquid into the added sterile water, mix well, dry and fix on the flame; Dye for 1 min, wash with water, and dry naturally; mordant with iodine solution for 1 min, wash with water, and dry; decolorize with 95% ethanol for 30 seconds, wash with water, and dry; co...

Embodiment 3

[0061] Example 3 Experiment of the effect of BY08 dissolving potassium feldspar under the culture of potassium-dissolving liquid medium

[0062] (1) Medium preparation:

[0063] ①Potassium solution liquid medium: glucose 5.0g, magnesium sulfate heptahydrate 0.5g, calcium carbonate 0.1g, ferric chloride 0.005g, tricalcium phosphate 2.0g, potassium feldspar 2.0g, distilled water 1L, pH 7.0-7.5 .

[0064] ②LB liquid medium: peptone 10.0g, yeast extract powder 5.0g, sodium chloride 10.0g, distilled water 1L, pH7.0-7.5.

[0065] ③ LB solid medium: peptone 10.0g, yeast extract powder 5.0g, sodium chloride 10.0g, distilled water 1L, agar 18.0g, pH7.0-7.5.

[0066] (2) Preparation of test solution:

[0067] Pick BY08 bacteria in glycerol and streak on LB solid medium plate, culture at 37°C for 12h. Put BY08 on the plate into a test tube containing LB liquid medium, culture at 37°C, 180rpm, on a shaker for 12h. Transfer the culture medium to fresh LB liquid medium, and shake it to...

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Abstract

The invention discloses bacillus bataviensis BY08 and application thereof, belongs to the technical field of microorganisms, and aims to provide a strain which can dissolve insoluble mineral potassiumfeldspar, release water-soluble potassium, and improve the utilization rate of plants on insoluble potassium in soil to promote plant growth, can significantly inhibit pseudomonas solanacearum, has significant resistance to Cu (II), Zn (II), Mn (II) and Pb (II) metals, and has hemolysis-negative safety; the collection number of the strain is CGMCC No.20024, and the strain is collected in China General Microbiological Culture Collection Center.

Description

technical field [0001] The invention discloses a strain of Bacillus bataviensis, and also relates to the application of the Bacillus bataviensis, belonging to the technical field of microorganisms. Background technique [0002] Potassium is one of the three major nutrients necessary for crop growth and development. Potassium deficiency will affect plant growth, root development, and resistance to pests and diseases. Although the content of potassium in the soil is rich, 98% of it exists in mineral forms such as potassium feldspar and mica, and only available potassium that does not exceed 2% of the total soil potassium is in the form that plants can directly absorb and utilize (Malinovskaya IM, et al. , 1990; Gold-stein AH et al., 1994). Using microorganisms to dissolve mineral potassium in soil is an economical, effective and environmentally friendly measure to solve the problem of soil potassium deficiency, which is of great significance to the development of ecological a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P21/00A01P1/00B09C1/10C05F11/08C05G3/80C12R1/07
CPCC12N1/20A01N63/22B09C1/10C05F11/08C05G3/80C12R2001/07C12N1/205
Inventor 蔡燕飞刘晓玉王玉琪盛锡兴陈火君
Owner SOUTH CHINA AGRI UNIV
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