Orthogonal up-conversion luminescence nanoprobe, and preparation method and application thereof

A nano-probe and probe technology, applied in luminescent materials, nanotechnology, nano-optics, etc., can solve the problems of self-luminescence interference of biological samples, poor membrane permeability, photobleaching obstruction, etc., to eliminate self-luminescence interference, toxicity, etc. The effect of low, good photochemical stability

Inactive Publication Date: 2021-02-12
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The currently used down-converting organic fluorophores, quantum dots or lanthanide chelates are limited by the sensitivity of the fluorescent carrier to the environment, such as pH value and hydrophobicity, when detected in living cells and in vivo; Interference of sample autoluminescence, poor membrane permeability, hindrance of photobleaching

Method used

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  • Orthogonal up-conversion luminescence nanoprobe, and preparation method and application thereof
  • Orthogonal up-conversion luminescence nanoprobe, and preparation method and application thereof
  • Orthogonal up-conversion luminescence nanoprobe, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The orthogonal upconversion nanoprobe provided in this example is an upconversion nanocomposite particle with the above conversion nanoparticle as the core, adsorption of RdMs and surface modification dye Cy5.5, that is, UCNPs@mSiO 2 @RdMs @Cy5.5-pep nanoparticles. The UCNPs@mSiO2@RdMs@Cy5.5-pep up-conversion nanoparticles emit red light or green light in either of the two bands under the excitation of two different near-infrared lights, that is, a near-infrared excitation light It can correspondingly excite light of one wavelength band: it emits red light under the excitation of near-infrared light at 980nm, and emits green light under the excitation of near-infrared light at 808nm.

[0048] A method for preparing the aforementioned orthogonal up-conversion nanoprobes, comprising the following steps:

[0049] (1) Orthogonal upconversion nanoparticles with a three-layer core-shell structure are prepared by seed crystal method: the nanoparticles are NaYF doped with Yb, ...

Embodiment 2

[0072] The orthogonal up-conversion nanoprobe, preparation method and application provided in this example are basically the same as those in Example 1, except that:

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Abstract

The invention discloses an orthogonal up-conversion nano probe, which is an up-conversion nano composite particle, namely a UCNPs@mSiO2@RdMs@Cy5.5-pep nano particle, which takes up-conversion nano particles as a core and adsorbs RdMs while the surface of the up-conversion nano composite particle is modified by a dye Cy5.5. The nanoprobe can emit red light and green light respectively under the excitation of near-infrared light with the wavelengths of 980nm and 808nm at the same time. The invention also discloses a preparation method of the orthogonal upconversion nanoprobe. The preparation method comprises the following steps: preparing orthogonal upconversion nanoparticles with a three-layer core-shell structure; coating the surfaces of the orthogonal up-conversion nanoparticles with a layer of mesoporous silicon, and then performing modification to obtain UCNPs@mSiO2-NH2 nanoparticles; adsorbing RdMs in mesoporous channels of the particles to obtain UCNPs@mSiO2@RdMs; modifying cy5.5on the surfaces of the UCNPs@mSiO2@RdMs composite nanoparticles, and preparing the UCNPs@mSiO2@Cy5.5-pep nanoparticles. The invention further discloses application of the probe, nitric oxide and Caspase-3 protease are detected at the same time through green light quenching and red light recovery, and NO can be proved to be capable of promoting Caspase-3 up-regulation in a cell environment.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to an orthogonal up-conversion luminescent nanoprobe, its preparation method and detection in solution and cell environment. Background technique [0002] In the prior art, nitric oxide (nitric oxide, NO) is an important signaling molecule in cells, which is widely distributed in various tissues in the body and plays an important role in cardiovascular and cerebrovascular regulation, neural regulation, immune regulation, etc. biological effect. As a gas free radical, NO can rapidly diffuse in cells and tissues and react with biological targets. It is very difficult to detect the formation and migration of NO in vivo. Caspases, as an important class of human proteases, play an important role in the initiation and execution of apoptosis (also known as programmed cell death), and are an active regulatory pathway for cell growth and proliferation. Caspases are improperly regulated ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/85B82Y20/00B82Y30/00B82Y40/00G01N21/64
CPCC09K11/02C09K11/7773B82Y20/00B82Y30/00B82Y40/00G01N21/6428G01N2021/6432
Inventor 刘金亮王甜甜张勇张菁
Owner SHANGHAI UNIV
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