Compound Olerafuran A in herba portulacae as well as extraction and separation method and application of compound Olerafuran A

A separation method and compound technology, applied in the direction of food ingredients containing natural extracts, applications, drug combinations, etc., can solve the problem of low structural novelty, and achieve the effect of environmentally friendly process methods, simple and fast operation methods

Active Publication Date: 2021-02-26
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the chemical components isolated from purslane are known, and the structural novelty is low. Therefore, the development and isolation of new compounds in purslane are urgently needed

Method used

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  • Compound Olerafuran A in herba portulacae as well as extraction and separation method and application of compound Olerafuran A
  • Compound Olerafuran A in herba portulacae as well as extraction and separation method and application of compound Olerafuran A
  • Compound Olerafuran A in herba portulacae as well as extraction and separation method and application of compound Olerafuran A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Extracting and separating compound Olerafuran A from purslane and its extraction and separation method

[0034] A compound Olerafuran A extracted and isolated from purslane, the molecular formula is C 18 h 30 o 5 , named Olerafuran A, the chemical structure is:

[0035] Described new compound is called OlerafuranA according to structure, and table 1 is the NMR data of this new compound: 1 H-NMR with 13 C-NMR in DMSO.

[0036] Table 1. NMR data of the new compounds of the present invention.

[0037]

[0038] Olerafuran A: yellow oil, easily soluble in methanol, slightly soluble in chloroform. UV(MeOH)λ max :213nm. IR(KBr)v max 3404, 2983, 2928, 2854, 1763, 1736, 1468, 1443, 1378, 1252, 1178, 768cm -1 . HR-ESI(+)-TOF-MS gave m / z: 327.2168, [M+H] + The quasi-molecular ion peak has a molecular weight of 327.2167. to combine 1 H-NMR, 13 According to C-NMR and DEPT data, it is speculated that the possible molecular formula of the compound is C ...

Embodiment 2

[0047] Example 2 Anticholinesterase effect of compounds of the present invention.

[0048] 1. Main materials.

[0049] 1.1. Drugs and reagents.

[0050] The compound Olerafuran A used in the experiment was prepared by the above method with a purity of 90-99%. Sodium dihydrogen phosphate, disodium hydrogen phosphate (Sinopharm Chemical Reagent Co., Ltd.), physostigmine (Hanxiang Biotechnology), phosphorus-5,5 '-Dithiobisnitrobenzoic acid (Dithiobisnitrobenzoic acid, DTNB, Shanghai Jinsui Biotechnology Co., Ltd.), acetylcholinesterase (AChE) and iodide thioacetylcholine (Acetylthiocholine iodide, ATCI, Dalian Meilun Biotechnology Co., Ltd).

[0051] 1.2 Grouping.

[0052] Divided into negative control group, positive control group and experimental group, each group.

[0053] 2 Experimental methods.

[0054] 2.1 Sample preparation.

[0055] Precisely weigh 1 mg of samples Olerafuran A and physostigmine, respectively, and use methanol as a solvent to prepare five gradient co...

Embodiment 3

[0062] Example 3 Anti-tumor effect of the new compound of the present invention.

[0063] 1 main material.

[0064] 1.1 Drugs and reagents.

[0065] The new compound Olerafuran A used in the experiment was prepared by the above-mentioned method with a purity of 90-99%. It was accurately weighed and diluted with DMSO to the solution required for each dosage group below. DMEM high-glucose medium, fetal bovine serum (Hyclone Company of the United States); penicillin and streptomycin (Hangzhou Sijiqing Company).

[0066] 1.2 Cell lines.

[0067] Human colon cancer cell Caco-2, human breast cancer cell MCF-7, human gastric cancer cell BGC-823, human lung adenocarcinoma cell SPC-A1, human liver cancer cell BEL-7402, human cervical cancer cell Hela-229, ovarian cancer cell Ho-8910, human oral epidermoid carcinoma cells KB (Shanghai Cell Bank, Chinese Academy of Sciences).

[0068] 1.3 Grouping.

[0069] Divided into control group, experimental group and zero adjustment group (cu...

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Abstract

The invention relates to the field of traditional Chinese medicine extraction and separation, in particular to a new compound extracted, separated and identified from herba portulacae medicinal material as well as an extraction and separation method and application thereof. The invention provides a compound Olerafuran A extracted from herba portulacae as well as an extraction and separation methodand application of the compound Olerafuran A. The ester compound is prepared by sequentially adopting alcohol decoction extraction, silica gel column chromatography, polyamide column chromatography,ODS medium-pressure column and Sephadex LH-20 purification and liquid phase separation, the extraction and separation method is simple, convenient, rapid and environment-friendly, and the compound separated by the method is relatively high in purity. Pharmacological experiments prove that the obtained compound has anti-tumor and anti-cholinesterase effects, so that the compound Olerafuran A extracted from herba portulacae as well as a salt and a derivative thereof can be used as natural products to develop new traditional Chinese medicine and have a wide medical application prospect.

Description

technical field [0001] The present invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to a new compound extracted, separated and identified from purslane medicinal material and its extraction and separation method and application, specifically a compound Olerafuran A in purslane and its extraction and separation method and uses. Background technique [0002] Purslane (Portulaca oleracea L.), also known as longevity dish, horse amaranth, is a plant of the family Amaranthaceae. Purslane is drought and waterlogging resistant, light and shade resistant, widely distributed, and rich in resources. It has attracted much attention as a wild plant for both medicine and food. The 2015 edition of "Pharmacopia of the People's Republic of China" recorded the dry aerial parts of purslane as medicine. , has the functions of clearing away heat and detoxification, cooling blood to stop bleeding, and stopping dysentery. [0003] Moder...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/60A61P35/00A61P21/04A61P27/06A61P25/28A23L33/105A23L33/10
CPCC07D307/60A61P35/00A61P21/04A61P27/06A61P25/28A23L33/105A23L33/10A23V2002/00A23V2200/30A23V2200/308A23V2250/21
Inventor 英锡相刘锡龙兰秀娟
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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